The hydroxyl radical (•OH) is the most reactive oxygen species (ROS), and plays a significant role in a variety of diseases. In general, hydroxyl radical is considered to be a harmful by-product of oxidative metabolism, which can cause molecular damage in a living system. Therefore, sensitive and selective detection of intracellular hydroxyl radical is essential to understand cellular redox and the impact of its dysregulation on various pathologies. Although a variety of fluorescent sensors have been developed to detect hydroxyl radicals, their rapid photobleaching, short emission wavelengths and non-selective reactions with other ROS species have limited their applications in cells and tissues. To address this unmet need, we have developed a novel fluorescent probe to selectively detect intracellular hydroxyl radical in living cells.
The novel hydroxyl radical probe (
MitoROS™ OH580) is live-cell permeant and can rapidly generate red fluorescence when it reacts with •OH in cells. In the absence of •OH, the probe has no significant absorption and negligible fluorescence. This fluorogenic characteristic makes this new probe have high signal-to-noise ratio. The newly developed fluorescent probe also has high selectivity for •OH over other reactive oxygen species (ROS) and reactive nitrogen species (RNS), enabling the specific detection in a biological system. We anticipate that MitoROS™ OH580 can serve as a valuable tool for life science research and medical diagnostic applications.
Hydroxyl Radical Generation
Imaging Hydroxyl Radical in Living Cells
Representative Data of ROS Reagents in Living Cells
Representative Data of RNS Reagents in Living Cells
Probes for imaging ROS and RNS species in living cells
Intracellular ROS detection reagents and assays.
Selective ROS detection reagents and assays.