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ROS Brite™ 700 *Optimized for in Vivo Imaging*

Fluorescence response of ROS Brite™ 700 to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 670/700 nm.
Fluorescence response of ROS Brite™ 700 to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 670/700 nm.
Fluorescence response of ROS Brite™ 700 to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 670/700 nm.
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Physical properties
Molecular weight1295.14
Spectral properties
Excitation (nm)682
Emission (nm)701
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen. Examples include superoxide, hydroxyl radical, singlet oxygen and peroxides. ROS is highly reactive due to the presence of unpaired valence shell electrons. ROS forms as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis. However, during times of environmental stress (e.g., UV or heat exposure), ROS levels can increase dramatically. This may result in significant damage to cell structures. Cumulatively, this is known as oxidative stress. ROS are also generated by exogenous sources such as ionizing radiation. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in subsequent alteration of membrane lipids, proteins, and nucleic acids. Oxidative damage of these biomolecules is associated with aging as well as with a variety of pathological events, including atherosclerosis, carcinogenesis, ischemic reperfusion injury, and neurodegenerative disorders. ROS Brite™ 700 reagent is a new fluorogenic probe to measure oxidative stress in small animals. The cell-impermeant ROS Brite™ 700 reagent is a water-soluble nonfluorescent and produces bright near-infrared fluorescence upon ROS oxidation. The resulting fluorescence can be measured using in vivo fluorescence imaging. For cell-based ROS detection, please use our cell-permemeant ROS Brite™ 570, 670 and 780 reagents.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ROS Brite™ 700 *Optimized for in Vivo Imaging* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM77.212 µL386.059 µL772.117 µL3.861 mL7.721 mL
5 mM15.442 µL77.212 µL154.423 µL772.117 µL1.544 mL
10 mM7.721 µL38.606 µL77.212 µL386.059 µL772.117 µL

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Spectral properties

Excitation (nm)682
Emission (nm)701



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