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ROS Brite™ 700 *Optimized for in Vivo Imaging*

Fluorescence response of ROS Brite™ 700 to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 670/700 nm.
Fluorescence response of ROS Brite™ 700 to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 670/700 nm.
Fluorescence response of ROS Brite™ 700 to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 670/700 nm.
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Physical properties
Molecular weight1295.14
SolventDMSO
Spectral properties
Excitation (nm)682
Emission (nm)701
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
1295.14
Excitation (nm)
682
Emission (nm)
701
Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen. Examples include superoxide, hydroxyl radical, singlet oxygen and peroxides. ROS is highly reactive due to the presence of unpaired valence shell electrons. ROS forms as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis. However, during times of environmental stress (e.g., UV or heat exposure), ROS levels can increase dramatically. This may result in significant damage to cell structures. Cumulatively, this is known as oxidative stress. ROS are also generated by exogenous sources such as ionizing radiation. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in subsequent alteration of membrane lipids, proteins, and nucleic acids. Oxidative damage of these biomolecules is associated with aging as well as with a variety of pathological events, including atherosclerosis, carcinogenesis, ischemic reperfusion injury, and neurodegenerative disorders. ROS Brite™ 700 reagent is a new fluorogenic probe to measure oxidative stress in small animals. The cell-impermeant ROS Brite™ 700 reagent is a water-soluble nonfluorescent and produces bright near-infrared fluorescence upon ROS oxidation. The resulting fluorescence can be measured using in vivo fluorescence imaging. For cell-based ROS detection, please use our cell-permemeant ROS Brite™ 570, 670 and 780 reagents.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ROS Brite™ 700 *Optimized for in Vivo Imaging* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM77.212 µL386.059 µL772.117 µL3.861 mL7.721 mL
5 mM15.442 µL77.212 µL154.423 µL772.117 µL1.544 mL
10 mM7.721 µL38.606 µL77.212 µL386.059 µL772.117 µL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Excitation (nm)682
Emission (nm)701

Images


Citations


View all 13 citations: Citation Explorer
Bone microenvironment regulative hydrogels with ROS scavenging and prolonged oxygen-generating for enhancing bone repair
Authors: Sun, Han and Xu, Juan and Wang, Yangyufan and Shen, Siyu and Xu, Xingquan and Zhang, Lei and Jiang, Qing
Journal: Bioactive Materials (2023): 477--496
Nanomodulators Capable of Timely Scavenging ROS for Inflammation and Prognosis Control Following Photothermal Tumor Therapy
Authors: Wang, Shuai and Huang, Jixi and Zhu, Hanyin and Zhu, Jing and Wang, Zhenqiang and Xing, Yuxin and Xie, Xiyue and Cai, Kaiyong and Zhang, Jixi
Journal: Advanced Functional Materials (2023): 2213151
N-cystaminylbiguanide MC001 prevents neuron cell death and alleviates motor deficits in the MPTP-model of Parkinson’s disease
Authors: Xu, Binglin and Wang, Xiaoquan and Xu, Zhengshuang and Li, Qinkai and Quan, Junmin
Journal: Neuroscience Letters (2022): 136751
Melanin Nanoparticle-Actuated Redox-State Perturbation and Temporally Photoactivated Thermal Stress for Synergistic Tumor Therapy
Authors: Zhu, Hanyin and Qu, Yongyi and Wang, Shuai and Huang, Jixi and Zhu, Jing and Wang, Lu and Cai, Kaiyong and Zhang, Jixi
Journal: ACS Biomaterials Science \& Engineering (2022): 3944--3956
Toxin-Enabled “On-Demand” Liposomes for Enhanced Phototherapy to Treat and Protect against Methicillin-Resistant Staphylococcus aureus Infection
Authors: Zhuge, Deli and Chen, Mengchun and Yang, Xuewei and Zhang, Xufei and Yao, Lulu and Li, Li and Wang, Haonan and Chen, Hao and Yin, Qingqing and Tian, Dongyan and others,
Journal: Small (2022): 2203292
Detection of Vascular Reactive Oxygen Species in Experimental Atherosclerosis by High-Resolution Near-Infrared Fluorescence Imaging Using VCAM-1-Targeted Liposomes Entrapping a Fluorogenic Redox-Sensitive Probe
Authors: Manea, Simona-Adriana and Vlad, Mihaela-Loredana and Rebleanu, Daniela and Lazar, Alexandra-Gela and Fenyo, Ioana Madalina and Calin, Manuela and Simionescu, Maya and Manea, Adrian
Journal: Oxidative Medicine and Cellular Longevity (2021)
Noninvasive Photochemical Sealing for Achilles Tendon Rupture by Combination of Upconversion Nanoparticles and Photochemical Tissue Bonding Technology
Authors: Zhu, Yiming and Xie, Aiguo and Li, Ming and Zhang, Chihao and Ni, Tao
Journal: BioMed Research International (2020)
The Ultrastructural and Abnormal Calcium Handling in Pulmonary Vein Sleeve Cells, Atrial and Ventricular Myocytes During Ageing
Authors: Masoud, Said
Journal: (2018)
Human elongation factor 4 regulates cancer bioenergetics by acting as a mitochondrial translation switch
Authors: Zhu, Ping and Liu, Yongzhang and Zhang, Fenglin and Bai, Xiufeng and Chen, Zilei and Shangguan, Fugen and Zhang, Bo and Zhang, Lingyun and Chen, Qianqian and Xie, Deyao and others,
Journal: Cancer research (2018): 2813--2824
Thiol-Mediated Synthesis of Hyaluronic Acid-Epigallocatechin-3-O-Gallate Conjugates for the Formation of Injectable Hydrogels with Free Radical Scavenging Property and Degradation Resistance
Authors: Liu, Chixuan and Bae, Ki Hyun and Yamashita, Atsushi and Chung, Joo Eun and Kurisawa, Motoichi
Journal: Biomacromolecules (2017)

References


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Lipoxin A inhibits porphyromonas gingivalis-induced aggregation and reactive oxygen species production by modulating neutrophil-platelet interaction and CD11b expression
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Regulation of cyclooxygenase-2 and cytosolic phospholipase A2 gene expression by lipopolysaccharide through the RNA-binding protein HuR: involvement of NADPH oxidase, reactive oxygen species and mitogen-activated protein kinases
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Journal: Br J Pharmacol (2011): 1691
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Journal: Theriogenology (2011): 951
Coenzyme Q functionalized CdTe/ZnS quantum dots for reactive oxygen species (ROS) imaging
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The role of reactive oxygen species in WP 631-induced death of human ovarian cancer cells: a comparison with the effect of doxorubicin
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