Name: ROS Brite™ HPF *Optimized for Detecting Reactive Oxygen Species (ROS)*
Brand: AAT Bioquest
SKU: 16051
Price: 174 USD
Availability: InStock

ROS Brite™ HPF

Optimized for Detecting Reactive Oxygen Species (ROS)

Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen. Examples include superoxide, hydroxyl radical, singlet oxygen and peroxides. ROS is highly reactive due to the presence of unpaired valence shell electrons. ROS forms as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis. However, during times of environmental stress (e.g., UV or heat exposure), ROS levels can increase dramatically. This may result in significant damage to cell structures. Cumulatively, this is known as oxidative stress. ROS are also generated by exogenous sources such as ionizing radiation. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in subsequent alteration of membrane lipids, proteins, and nucleic acids. Oxidative damage of these biomolecules is associated with aging as well as with a variety of pathological events, including atherosclerosis, carcinogenesis, ischemic reperfusion injury, and neurodegenerative disorders. ROS Brite™ HPF is a fluorogenic probe to measure hydroxyly radical in cells using conventional fluorescence microscopy, high-content imaging, microplate fluorometry, or flow cytometry. The cell-permeant ROS Brite™ HPF reagent is nonfluorescent and produces bright green fluorescence upon reaction with hydroxyl radical. The resulting fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry. In the presence of peroxidase, HPF also reacts with hydrogen peroxide. HPF has good selectivity to hydroxyl radical compared to other ROS (e.g., supoeroxide and nitric oxide). APF and HPF show relatively high resistance to light-induced oxidation. APF and HPF are nonfluorescent until they react with the hydroxyl radical or peroxynitrite anion.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
16051	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ROS Brite™ HPF *Optimized for Detecting Reactive Oxygen Species (ROS)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	235.627 µL	1.178 mL	2.356 mL	11.781 mL	23.563 mL
5 mM	47.125 µL	235.627 µL	471.254 µL	2.356 mL	4.713 mL
10 mM	23.563 µL	117.813 µL	235.627 µL	1.178 mL	2.356 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	424.4 g/mol	=	mL	x	mM	=	mmol

Citations

View all 8 citations: Citation Explorer

Ultrasmall metal alloy nanozymes mimicking neutrophil enzymatic cascades for tumor catalytic therapy

Authors:

Meng, Xiangqin and Fan, Huizhen and Chen, Lei and He, Jiuyang and Hong, Chaoyi and Xie, Jiaying and Hou, Yinyin and Wang, Kaidi and Gao, Xingfa and Gao, Lizeng and others,

Journal:

Nature Communications (2024): 1626

Material-engineered bioartificial microorganisms enabling efficient scavenging of waterborne viruses

Authors:

Li, Huixin and Xu, Yanpeng and Wang, Yang and Cui, Yihao and Lin, Jiake and Zhou, Yuemin and Tang, Shuling and Zhang, Ying and Hao, Haibin and Nie, Zihao and others,

Journal:

Nature Communications (2023): 4658

Material-Engineered Bioartificial Microorganisms Enable Efficient Waterborne Viruses Scavenging

Authors:

Li, Huixin and Cui, Yihao and Lin, Jiake and Zhang, Ying and Hao, Haibin and Wang, Yang and Xu, Yan and Nie, Zi-Hao and Zhou, Yuemin and Wang, Xiao-Yu and others,

Toxin-Enabled “On-Demand” Liposomes for Enhanced Phototherapy to Treat and Protect against Methicillin-Resistant Staphylococcus aureus Infection

Authors:

Zhuge, Deli and Chen, Mengchun and Yang, Xuewei and Zhang, Xufei and Yao, Lulu and Li, Li and Wang, Haonan and Chen, Hao and Yin, Qingqing and Tian, Dongyan and others,

Journal:

Small (2022): 2203292

Molecular Hydrogen Attenuates High Hydrostatic Pressure-Induced Neuronal Cell Damage by Reversing Dysfunction of Mitochondrial Electron Transfer Chain

Authors:

Lu, Zhuoyang and Zhang, Tiantian and Hu, Yachong and Liu, Hui and Cui, Li and Long, Jiangang and Liu, Jiankang

References

View all 22 references: Citation Explorer

Developmental toxicity evaluation of three hexabromocyclododecane diastereoisomers on zebrafish embryos

Authors:

Du M, Zhang D, Yan C, Zhang X.

Journal:

Aquat Toxicol (2012): 1

MAPK inhibitors and siRNAs differentially affect cell death and ROS levels in arsenic trioxide-treated human pulmonary fibroblast cells

Authors:

Park WH., undefined

Journal:

Oncol Rep (2012): 1611

MG132, a proteasome inhibitor, induces human pulmonary fibroblast cell death via increasing ROS levels and GSH depletion

Authors:

Park WH, Kim SH.

Journal:

Oncol Rep (2012): 1284

Gallic acid-induced lung cancer cell death is accompanied by ROS increase and glutathione depletion

Authors:

You BR, Kim SZ, Kim SH, Park WH.

Journal:

Mol Cell Biochem (2011): 295

Proteasome inhibition by MG132 induces growth inhibition and death of human pulmonary fibroblast cells in a caspase-independent manner

Authors:

You BR, Park WH.

Journal:

Oncol Rep (2011): 1705

Physical properties

Molecular weight	424.40
Solvent	DMSO

Spectral properties

Excitation (nm)	498
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black
Instrument specification(s)	Bottom read mode

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2025