ROS Brite™ APF *Optimized for Detecting Reactive Oxygen Species (ROS)*

ROS Brite™ APF

Optimized for Detecting Reactive Oxygen Species (ROS)

Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen. Examples include superoxide, hydroxyl radical, singlet oxygen and peroxides. ROS is highly reactive due to the presence of unpaired valence shell electrons. ROS forms as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis. However, during times of environmental stress (e.g., UV or heat exposure), ROS levels can increase dramatically. This may result in significant damage to cell structures. Cumulatively, this is known as oxidative stress. ROS are also generated by exogenous sources such as ionizing radiation. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in subsequent alteration of membrane lipids, proteins, and nucleic acids. Oxidative damage of these biomolecules is associated with aging as well as with a variety of pathological events, including atherosclerosis, carcinogenesis, ischemic reperfusion injury, and neurodegenerative disorders. ROS Brite™ APF is a fluorogenic probe to measure hydroxyly radical in cells using conventional fluorescence microscopy, high-content imaging, microplate fluorometry, or flow cytometry. The cell-permeant ROS Brite™ APF reagent is nonfluorescent and produces bright green fluorescence upon reaction with hydroxyl radical. The resulting fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry. In the presence of peroxidase, APF also reacts with hydrogen peroxide. APF has good selectivity to hydroxyl radical compared to other ROS. APF and HPF show relatively high resistance to light-induced oxidation. APF and HPF are nonfluorescent until they react with the hydroxyl radical or peroxynitrite anion. APF will also react with the hypochlorite anion.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
16050	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ROS Brite™ APF *Optimized for Detecting Reactive Oxygen Species (ROS)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	236.172 µL	1.181 mL	2.362 mL	11.809 mL	23.617 mL
5 mM	47.234 µL	236.172 µL	472.344 µL	2.362 mL	4.723 mL
10 mM	23.617 µL	118.086 µL	236.172 µL	1.181 mL	2.362 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	423.42 g/mol	=	mL	x	mM	=	mmol

Citations

View all 11 citations: Citation Explorer

PGRN protects against serum deprivation-induced cell death by encouraging the ROS scavenger system in cervical cancer

Authors:

Lu, Yi and Feng, Tingting and Xu, Xiaoying and Wang, Xiao and Tang, Wei

Ultrasmall metal alloy nanozymes mimicking neutrophil enzymatic cascades for tumor catalytic therapy

Authors:

Meng, Xiangqin and Fan, Huizhen and Chen, Lei and He, Jiuyang and Hong, Chaoyi and Xie, Jiaying and Hou, Yinyin and Wang, Kaidi and Gao, Xingfa and Gao, Lizeng and others,

Journal:

Nature Communications (2024): 1626

Hemithioindigo-Based Visible Light-Activated Molecular Machines Kill Bacteria by Oxidative Damage

Authors:

Santos, Ana L and van Venrooy, Alexis and Reed, Anna K and Wyderka, Aaron M and Garc{\'\i}a-L{\'o}pez, V{\'\i}ctor and Alemany, Lawrence B and Oliver, Antonio and Tegos, George P and Tour, James M

Journal:

Advanced Science (2022): 2203242

Toxin-Enabled “On-Demand” Liposomes for Enhanced Phototherapy to Treat and Protect against Methicillin-Resistant Staphylococcus aureus Infection

Authors:

Zhuge, Deli and Chen, Mengchun and Yang, Xuewei and Zhang, Xufei and Yao, Lulu and Li, Li and Wang, Haonan and Chen, Hao and Yin, Qingqing and Tian, Dongyan and others,

Journal:

Small (2022): 2203292

Fluorescent Nanogel Sensors for X-ray Dosimetry

Authors:

Jiang, Li and Li, Wenxiang and Nie, Jing and Wang, Rensheng and Chen, Xinjian and Fan, Wenhui and Hu, Liang

Journal:

ACS sensors (2021): 1643--1648

References

View all 22 references: Citation Explorer

Developmental toxicity evaluation of three hexabromocyclododecane diastereoisomers on zebrafish embryos

Authors:

Du M, Zhang D, Yan C, Zhang X.

Journal:

Aquat Toxicol (2012): 1

MAPK inhibitors and siRNAs differentially affect cell death and ROS levels in arsenic trioxide-treated human pulmonary fibroblast cells

Authors:

Park WH., undefined

Journal:

Oncol Rep (2012): 1611

MG132, a proteasome inhibitor, induces human pulmonary fibroblast cell death via increasing ROS levels and GSH depletion

Authors:

Park WH, Kim SH.

Journal:

Oncol Rep (2012): 1284

Enhancement of gallic acid-induced human pulmonary fibroblast cell death by N-acetyl cysteine and L-buthionine sulfoximine

Authors:

You BR, Park WH.

Journal:

Hum Exp Toxicol (2011): 992

Proteasome inhibition by MG132 induces growth inhibition and death of human pulmonary fibroblast cells in a caspase-independent manner

Authors:

You BR, Park WH.

Journal:

Oncol Rep (2011): 1705

Physical properties

Molecular weight	423.42
Solvent	DMSO

Spectral properties

Excitation (nm)	498
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom
Instrument specification(s)	FITC filter set

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2025