Cyanine 5 monosuccinimidyl ester [equivalent to Cy5® NHS ester]

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HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by AAT’s Cy5<sup>®</sup> goat anti-mouse IgG conjugate (Red, Left) or Jackson’s Cy5<sup>®</sup> goat anti-mouse IgG conjugate (Red, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat# 17530).
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Unit Size: Cat No: Price (USD): Qty:
1 mg 151 $95

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Telephone: 1-800-990-8053
Fax: 1-408-733-1304
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Ex/Em (nm)650/669
Quantum Yield0.27
Extinction Coefficient
(cm-1 M-1)
Storage Freeze (<-15 °C)
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Category Biomolecule Labeling,
Chemical Building Blocks and Precursors
A variety of cyanine 5 ( Cy5®) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy5® dyes are one type of the most common red fluorophores. These versatile fluorophores can tolerate a pH range of 3-10 for use in a variety of applications at biologically relevant pHs. The dyes are also DMSO tolerant and photostable to enable transfer from storage to assay without loss of performance. The aqueous solubility eliminates the need for organic solvents in the assay buffers. Our Cy5® Fluors are thoroughly QC tested to ensure high levels of chromophore and reactive dye content. Mono-reactive dyes are suitable for targeted, precise labeling of proteins and oligonucleotides and bis-reactive dyes are more suitable for general labeling. NHS ester dyes are recommended for labeling amine groups and maleimide dyes are recommended for labeling thiol groups. This Cy5® NHS ester readily reacts with amino groups. AAT Bioquest offers Cy dye NHS esters in the form of triethylammonium salts that are more soluble in DMSO and DMF than the corresponding potassium salts that are offered by some other vendors. The Cy dye triethylammonium salts have the same reactivity and give the conjugates identical to the the Cy dye potassium salts. Cy5® is the trademark of GE Healthcare.

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cyanine 5 monosuccinimidyl ester [equivalent to Cy5® NHS ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
/ = x =

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Wavelength (nm)

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