Cy7 NHS Ester | AAT Bioquest

Cyanine 7 monosuccinimidyl ester

equivalent to Cy7® NHS ester

Cy7 NHS Ester is a near-infrared fluorescent dye (Ex/Em: 756/779 nm) for amine-reactive labeling that enables deep tissue imaging and whole-animal fluorescence detection with minimal autofluorescence interference.

Near-Infrared Emission (756/779 nm): Enables whole-animal imaging with minimal tissue absorption and background
Multimodal Imaging Compatibility: Suitable for fluorescence tomography, surgical guidance, and lymph node mapping
Enhanced Aqueous Solubility: Triethylammonium salt formulation improves reconstitution compared to potassium salts

Cy7 spectrum. Cy7 (Cyanine-7) is a fluorescent compound with an excitation peak at 756 nm and an emission peak at 779 nm. Other spectra of interest include: Cy5.5 (Cyanine-5.5), Cy5.5NS, and Cy3.5 (Cyanine-3.5). Cy7 (Cyanine-7) belongs to the following categories: Cyanines.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
161	1 mg	Price

Overview

Cy7 (Cyanine-7) is a fluorescent compound with an excitation peak at 756 nm and an emission peak at 779 nm.

A variety of cyanine 7 (Cy7®) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy7® dye conjugates are one type of the most common near infrared red fluorophores used in in vivo imaging applications. Cy7® NHS ester readily reacts with amino groups. AAT Bioquest offers Cy dye NHS esters in the form of triethylammonium salts that are more soluble in DMSO and DMF than the corresponding potassium salts that are offered by some other vendors. The Cy dye triethylammonium salts have the same reactivity and give the conjugates identical to the the Cy dye potassium salts. Cy7® is the trademark of GE Healthcare.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cyanine 7 monosuccinimidyl ester [equivalent to Cy7® NHS ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	113.493 µL	567.466 µL	1.135 mL	5.675 mL	11.349 mL
5 mM	22.699 µL	113.493 µL	226.986 µL	1.135 mL	2.27 mL
10 mM	11.349 µL	56.747 µL	113.493 µL	567.466 µL	1.135 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	881.11 g/mol	=	mL	x	mM	=	mmol

Citations

View all 19 citations: Citation Explorer

A broadly applicable protein-polymer adjuvant system for antiviral vaccines

Authors:

Wang, Caiqian and Geng, Yuanyuan and Wang, Haoran and Ren, Zeheng and Hou, Qingxiu and Fang, An and Wu, Qiong and Wu, Liqin and Shi, Xiujuan and Zhou, Ming and others,

Journal:

EMBO Molecular Medicine (2024): 1--33

Highly sensitive magnetic particle imaging of vulnerable atherosclerotic plaque with active myeloperoxidase-targeted nanoparticles

Authors:

Tong, Wei and Hui, Hui and Shang, Wenting and Zhang, Yingqian and Tian, Feng and Ma, Qiang and Yang, Xin and Tian, Jie and Chen, Yundai

Journal:

Theranostics (2021): 506

Highly sensitive magnetic particle imaging of vulnerable atherosclerotic plaque

Authors:

Tong, Wei and Hui, Hui and Shang, Wenting and Zhang, Yingqian and Tian, Feng and Ma, Qiang and Yang, Xin and Tian, Jie and Chen, Yundai

Sight and switch off: Nerve density visualization for interventions targeting nerves in prostate cancer

Authors:

You, Huijuan and Shang, Wenting and Min, Xiangde and Weinreb, Jeffrey and Li, Qiubai and Leapman, Michael and Wang, Liang and Tian, Jie

Journal:

Science Advances (2020): eaax6040

Near-Infrared Dual-Emission Ratiometric Fluorescence Imaging Nanoprobe for Real-Time Tracing the Generation of Endogenous Peroxynitrite in Single Living Cells and In Vivo

Authors:

Lin, Pengxiang and Chen, Dongxia and Zhang, Liangliang and Xu, Jiayao and Huang, Yong and Zhao, Shulin

Journal:

ACS Omega (2020)

References

View all 21 references: Citation Explorer

Excitation of Cy5 in self-assembled lipid bilayers using optical microresonators

Authors:

Freeman LM, Li S, Dayani Y, Choi HS, Malmstadt N, Armani AM.

Journal:

Appl Phys Lett (2011): 143703

Theranostic cRGD-BioShuttle Constructs Containing Temozolomide- and Cy7 For NIR-Imaging and Therapy

Authors:

Wiessler M, Hennrich U, Pipkorn R, Waldeck W, Cao L, Peter J, Ehemann V, Semmler W, Lammers T, Braun K.

Journal:

Theranostics (2011): 381

Rational approach to select small peptide molecular probes labeled with fluorescent cyanine dyes for in vivo optical imaging

Authors:

Berezin MY, Guo K, Akers W, Livingston J, Solomon M, Lee H, Liang K, Agee A, Achilefu S.

Journal:

Biochemistry (2011): 2691

In vivo detection of embryonic stem cell-derived cardiovascular progenitor cells using Cy3-labeled Gadofluorine M in murine myocardium

Authors:

Adler ED, Bystrup A, Briley-Saebo KC, Mani V, Young W, Giovanonne S, Altman P, Kattman SJ, Frank JA, Weinmann HJ, Keller GM, Fayad ZA.

Journal:

JACC Cardiovasc Imaging (2009): 1114

Quantitative proteomics by fluorescent labeling of cysteine residues using a set of two cyanine-based or three rhodamine-based dyes

Authors:

Volke D, Hoffmann R.

Journal:

Electrophoresis (2008): 4516

Physical properties

Molecular weight	881.11
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.05
Correction factor (280 nm)	0.036
Correction factor (482 nm)	0.0005
Correction factor (565 nm)	0.0193
Correction factor (650 nm)	0.165
Extinction coefficient (cm ^-1 M ^-1)	250000
Excitation (nm)	756
Emission (nm)	779
Quantum yield	0.3

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	477908-53-5

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Page updated on September 11, 2024