Cyanines

Cyanine is the non-systematic name of a synthetic dye family belonging to the polymethine group. The word cyanin stems from the English word "cyan", which conventionally means a shade of blue-green. Cyanine dyes exhibit large molar absorptivities (~150,000 to 300,000 M^-1cm^-1) and moderate quantum yields resulting in extremely bright fluorescence signals. Cyanines have proven useful in several fields including photography, biology, laser technology and analytical chemistry. AAT Bioquest offers the largest collection of cyanine dyes that maybe useful as molecular probes for a variety of biological applications. For certain biological applications, cyanines can advantageously replace other conventional dyes such as the fluoresceins and rhodamines.

Cyanine Dyes: Cy3 and Cy5

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Among cyanine dyes, Cy3^® which fluoresces greenish yellow (~550 nm excitation, ~570 nm emission) and Cy5^® which is fluorescent in the red region (~650 nm excitation, ~670 nm emission) are the most popular. Because of their minimal spectral overlap, Cy3^® and Cy5^® are typically combined for 2 color detection. Cy3^® can be detected by various fluorometers, imagers, and microscopes with standard filters for TRITC. Due to its high molar extinction coefficient, Cy3^® dye is also easily detected by naked eye on electrophoresis gels, and in solution. Cy5^® became a popular replacement for far red fluorescent dyes because of its high extinction coefficient (as small as 1 nmol can be detected in gel electrophoresis by naked eye) and its fluorophore emission maximum in the red region, where many CCD detectors have maximum sensitivity and biological objects give low background interference.

Spectral Properties

1. ε: is the extinction coefficient (cm^-1M^-1).
2. Φ: is the fluorescence quantum yield.

Sulfonated vs Non-Sulfonated Cyanines

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Sulfonated and non-sulfonated cyanine dyes exhibit very similar fluorescent characteristics and for many applications they can be used interchangeably. The difference between the dyes is mainly in their solubility. Non-sulfonated cyanines must be dissolved in an organic co-solvent, such as DMF or DMSO, prior to labeling of a biomolecule. While sulfonated cyanines can be used in aqueous conditions (the charged sulfonate groups decrease dye and conjugate aggregation). For purification by dialysis against water or an aqueous buffer, sulfonated cyanines must be used for efficient removal of any unreacted dye. Purification by gel filtration, chromatography, or electrophoresis is adequate for both sulfonated and non-sulfonated cyanines.

	Cy3®		Cy5®		Cy7®
Form	Sulfo	Non-Sulfo	Sulfo	Non-Sulfo	Sulfo	Non-sulfo
Ex/Em (nm)	554/568		650/669		754/778
Filter Set	Cy/TRITC		Cy5		Cy7
ε	150,000	140,000	250,000	230,000	250,000	250,000
Φ	0.10	0.07	0.27	0.16	0.12	0.2
Cat No.	263	191	268	195	161	198

1. ε: is the extinction coefficient (cm^-1M^-1).
2. Φ: is the fluorescence quantum yield.

Cyanine Products

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We offer a broad selection of cyanine products including reactive dyes and conjugation kits for labeling biomolecules, conjugates, indicators and more.

• Cyanine antibody and protein labeling kits
• Annexin V conjugates for apoptosis detection
• Tandem dyes
• Cyanine dyes for 2-D Fluorescence Difference Gel Electrophoresis (DIGE)
• Cyanine labeled tyramide

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Cyanine Reactive Dyes

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Select a dye-labeling chemistry for modifying the following reactive groups.

Cyanine-Labeled Secondary Conjugates

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Select from a variety of cyanine labeled secondary antibody and streptavidin conjugates for signal amplification detection of primary antibodies.

Ordering Information

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