Cy3 tetrazine

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This inverse electron demand cycloaddition reaction has gained popularity due to the potential for extremely fast cycloaddition kinetics with TCO as the dienophile. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this poweful click reaction. Cy3-tetrazine has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. It is widely used for labeling peptides, proteins and oligos etc. Cy3 dyes have enhanced fluorescence upon binding to proteins.

Chemical structure for Cy3 tertrazine [Cy3 tertrazine]

Protocol

SDS

COA

Catalog	Size	Price	Quantity
910	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3 tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	110.124 µL	550.618 µL	1.101 mL	5.506 mL	11.012 mL
5 mM	22.025 µL	110.124 µL	220.247 µL	1.101 mL	2.202 mL
10 mM	11.012 µL	55.062 µL	110.124 µL	550.618 µL	1.101 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	908.07 g/mol	=	mL	x	mM	=	mmol

References

View all 49 references: Citation Explorer

Beyond click chemistry - supramolecular interactions of 1,2,3-triazoles

Authors:

Schulze B, Schubert US.

Journal:

Chem Soc Rev (2014): 2522

Calixarene-based chemosensors by means of click chemistry

Authors:

Song M, Sun Z, Han C, Tian D, Li H, Kim JS.

Journal:

Chem Asian J (2014): 2344

Use of click-chemistry in the development of peptidomimetic enzyme inhibitors

Authors:

Fabbrizzi P, Menchi G, Guarna A, Trabocchi A.

Journal:

Curr Med Chem (2014): 1467

Applications of copper-catalyzed click chemistry in activity-based protein profiling

Authors:

Martell J, Weerapana E.

Journal:

Molecules (2014): 1378

'Click chemistry' for diagnosis: a patent review on exploitation of its emerging trends

Authors:

M, undefined and hare A, Banerjee P, Bhutkar S, Hirwani R.

Journal:

Expert Opin Ther Pat (2014): 1287

Physical properties

Molecular weight	908.07
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.07
Correction factor (280 nm)	0.073
Extinction coefficient (cm ^-1 M ^-1)	150000 ¹
Excitation (nm)	555
Emission (nm)	569
Quantum yield	0.15 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on September 29, 2025