Cy3 tetrazine

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	908.07
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.07
Correction Factor (280 nm)	0.073
Extinction coefficient (cm ^-1 M ^-1)	150000¹
Excitation (nm)	555
Emission (nm)	569
Quantum yield	0.15¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Direct upgrades

iFluor® 555 Tetrazine

Overview SDS Protocol

Upgrade: iFluor® 555 Tetrazine

Molecular weight

908.07

Correction Factor (260 nm)

0.07

Correction Factor (280 nm)

0.073

Extinction coefficient (cm ^-1 M ^-1)

150000¹

Excitation (nm)

555

Emission (nm)

569

Quantum yield

0.15¹

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This inverse electron demand cycloaddition reaction has gained popularity due to the potential for extremely fast cycloaddition kinetics with TCO as the dienophile. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this poweful click reaction. Cy3-tetrazine has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. It is widely used for labeling peptides, proteins and oligos etc. Cy3 dyes have enhanced fluorescence upon binding to proteins.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3 tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	110.124 µL	550.618 µL	1.101 mL	5.506 mL	11.012 mL
5 mM	22.025 µL	110.124 µL	220.247 µL	1.101 mL	2.202 mL
10 mM	11.012 µL	55.062 µL	110.124 µL	550.618 µL	1.101 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.07
Correction Factor (280 nm)	0.073
Extinction coefficient (cm ^-1 M ^-1)	150000¹
Excitation (nm)	555
Emission (nm)	569
Quantum yield	0.15¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Cy3 phosphoramidite	555	569	150000¹	0.15¹	0.07	0.073
Cy3 tyramide	555	569	150000¹	0.15¹	0.07	0.073
Cy3 aldehyde	555	569	150000¹	0.15¹	0.07	0.073
Cy3B Tetrazine	560	571	120000¹	0.58¹	0.048	0.069
DBCO-Cy3	555	569	150000¹	0.15¹	0.07	0.073
Cy5 tetrazine	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
XFD488 tetrazine Same Structure to Alexa Fluor™ 488 tetrazine	490	520	71000	0.92¹	0.30	0.11

Images

Figure 1. Chemical structure for Cy3 tertrazine [Cy3 tertrazine]

Citations

View all 1 citations: Citation Explorer

Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene--methyltetrazine reactions
Authors: Muraki, Michiro and Hirota, Kiyonori
Journal: BMC biotechnology (2017): 56

References

View all 49 references: Citation Explorer

Beyond click chemistry - supramolecular interactions of 1,2,3-triazoles
Authors: Schulze B, Schubert US.
Journal: Chem Soc Rev (2014): 2522

Calixarene-based chemosensors by means of click chemistry
Authors: Song M, Sun Z, Han C, Tian D, Li H, Kim JS.
Journal: Chem Asian J (2014): 2344

Use of click-chemistry in the development of peptidomimetic enzyme inhibitors
Authors: Fabbrizzi P, Menchi G, Guarna A, Trabocchi A.
Journal: Curr Med Chem (2014): 1467

Applications of copper-catalyzed click chemistry in activity-based protein profiling
Authors: Martell J, Weerapana E.
Journal: Molecules (2014): 1378

'Click chemistry' for diagnosis: a patent review on exploitation of its emerging trends
Authors: M, undefined and hare A, Banerjee P, Bhutkar S, Hirwani R.
Journal: Expert Opin Ther Pat (2014): 1287

Specific and quantitative labeling of biomolecules using click chemistry
Authors: Horisawa K., undefined
Journal: Front Physiol (2014): 457

A dynamic duo: pairing click chemistry and postpolymerization modification to design complex surfaces
Authors: Arnold RM, Patton DL, Popik VV, Locklin J.
Journal: Acc Chem Res (2014): 2999

Applications of azide-based bioorthogonal click chemistry in glycobiology
Authors: Zhang X, Zhang Y.
Journal: Molecules (2013): 7145

Peptide conjugation via CuAAC 'click' chemistry
Authors: Ahmad Fuaad AA, Azmi F, Skwarczynski M, Toth I.
Journal: Molecules (2013): 13148

In situ click chemistry: from small molecule discovery to synthetic antibodies
Authors: Millward SW, Agnew HD, Lai B, Lee SS, Lim J, Nag A, Pitram S, Rohde R, Heath JR.
Journal: Integr Biol (Camb) (2013): 87