DBCO-Cy3

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Telephone	1-800-990-8053
Fax	1-800-609-2943
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Physical properties

Molecular weight	1185.56
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.07
Correction Factor (280 nm)	0.073
Extinction coefficient (cm ^-1 M ^-1)	150000¹
Excitation (nm)	555
Emission (nm)	569
Quantum yield	0.15¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DBCO-Cy3 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	84.348 µL	421.742 µL	843.483 µL	4.217 mL	8.435 mL
5 mM	16.87 µL	84.348 µL	168.697 µL	843.483 µL	1.687 mL
10 mM	8.435 µL	42.174 µL	84.348 µL	421.742 µL	843.483 µL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.07
Correction Factor (280 nm)	0.073
Extinction coefficient (cm ^-1 M ^-1)	150000¹
Excitation (nm)	555
Emission (nm)	569
Quantum yield	0.15¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Cy3 tetrazine	555	569	150000¹	0.15¹	0.07	0.073
DBCO-Cy5	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
Cy3 phosphoramidite	555	569	150000¹	0.15¹	0.07	0.073
Cy3 tyramide	555	569	150000¹	0.15¹	0.07	0.073
Cy3 aldehyde	555	569	150000¹	0.15¹	0.07	0.073
Cy3B DBCO	560	571	120000¹	0.58¹	0.048	0.069

Images

Figure 1. Chemical structure for DBCO-Cy3

Figure 2. (A) Strategy for ‘click’-capture of B2-N3 with DBCO-Cy3 (B) fluorescence microscopy of B2-N3 labelled Mycobacterium smegmatis with DBCO-Cy3 via SPAAC. Scale bars are 5 μm. Source: Imaging of antitubercular dimeric boronic acids at the mycobacterial cell surface by click-probe capture by Collette S. Guy, Ruben M. F. Tomás, Qiao Tang, Matthew I. Gibson and Elizabeth Fullam. Chem. Commun., Aug. 2022.

Citations

View all 2 citations: Citation Explorer

Imaging of antitubercular dimeric boronic acids at the mycobacterial cell surface by click-probe capture
Authors: Guy, Collette S and Tom{\'a}s, Ruben MF and Tang, Qiao and Gibson, Matthew I and Fullam, Elizabeth
Journal: Chemical Communications (2022): 9361--9364

Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene--methyltetrazine reactions
Authors: Muraki, Michiro and Hirota, Kiyonori
Journal: BMC biotechnology (2017): 56

References

View all 49 references: Citation Explorer

Beyond click chemistry - supramolecular interactions of 1,2,3-triazoles
Authors: Schulze B, Schubert US.
Journal: Chem Soc Rev (2014): 2522

Calixarene-based chemosensors by means of click chemistry
Authors: Song M, Sun Z, Han C, Tian D, Li H, Kim JS.
Journal: Chem Asian J (2014): 2344

Use of click-chemistry in the development of peptidomimetic enzyme inhibitors
Authors: Fabbrizzi P, Menchi G, Guarna A, Trabocchi A.
Journal: Curr Med Chem (2014): 1467

Applications of copper-catalyzed click chemistry in activity-based protein profiling
Authors: Martell J, Weerapana E.
Journal: Molecules (2014): 1378

'Click chemistry' for diagnosis: a patent review on exploitation of its emerging trends
Authors: M, undefined and hare A, Banerjee P, Bhutkar S, Hirwani R.
Journal: Expert Opin Ther Pat (2014): 1287

Specific and quantitative labeling of biomolecules using click chemistry
Authors: Horisawa K., undefined
Journal: Front Physiol (2014): 457

A dynamic duo: pairing click chemistry and postpolymerization modification to design complex surfaces
Authors: Arnold RM, Patton DL, Popik VV, Locklin J.
Journal: Acc Chem Res (2014): 2999

Applications of azide-based bioorthogonal click chemistry in glycobiology
Authors: Zhang X, Zhang Y.
Journal: Molecules (2013): 7145

Peptide conjugation via CuAAC 'click' chemistry
Authors: Ahmad Fuaad AA, Azmi F, Skwarczynski M, Toth I.
Journal: Molecules (2013): 13148

In situ click chemistry: from small molecule discovery to synthetic antibodies
Authors: Millward SW, Agnew HD, Lai B, Lee SS, Lim J, Nag A, Pitram S, Rohde R, Heath JR.
Journal: Integr Biol (Camb) (2013): 87