DBCO-Cy3
This azadibenzocyclooctyne-cyanine dye derivative is a versatile labeling reagent for detection of azide containing molecules or compounds. Cyclooctynes are useful in strain-promoted copper-free azide-alkyne cycloaddition reactions. This dibenzocyclooctyne will react with azide-functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage. AAT Bioquest offer a broad range of dye azide compounds (such as coumarin azides, fluorescein azides, rhodamine azides and cyanine azides) for click reactions.
![(A) Strategy for ‘click’-capture of B2-N3 with DBCO-Cy3 (B) fluorescence microscopy of B2-N3 labelled Mycobacterium smegmatis with DBCO-Cy3 via SPAAC. Scale bars are 5 μm. Source: <b>Imaging of antitubercular dimeric boronic acids at the mycobacterial cell surface by click-probe capture</b> by Collette S. Guy, Ruben M. F. Tomás, Qiao Tang, Matthew I. Gibson and Elizabeth Fullam. <em>Chem. Commun.</em>, Aug. 2022.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fdbco-cy3%2Ffigure-for-dbco-cy3_7qa2J.png&w=96&q=25)
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DBCO-Cy3 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 84.348 µL | 421.742 µL | 843.483 µL | 4.217 mL | 8.435 mL |
5 mM | 16.87 µL | 84.348 µL | 168.697 µL | 843.483 µL | 1.687 mL |
10 mM | 8.435 µL | 42.174 µL | 84.348 µL | 421.742 µL | 843.483 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
Cy3 tetrazine | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
DBCO-Cy5 | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
Cy3 phosphoramidite | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
Cy3 tyramide | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
Cy3 aldehyde | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
Cy3B DBCO | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
Citations
View all 2 citations: Citation Explorer
Imaging of antitubercular dimeric boronic acids at the mycobacterial cell surface by click-probe capture
Authors: Guy, Collette S and Tom{\'a}s, Ruben MF and Tang, Qiao and Gibson, Matthew I and Fullam, Elizabeth
Journal: Chemical Communications (2022): 9361--9364
Authors: Guy, Collette S and Tom{\'a}s, Ruben MF and Tang, Qiao and Gibson, Matthew I and Fullam, Elizabeth
Journal: Chemical Communications (2022): 9361--9364
Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene--methyltetrazine reactions
Authors: Muraki, Michiro and Hirota, Kiyonori
Journal: BMC biotechnology (2017): 56
Authors: Muraki, Michiro and Hirota, Kiyonori
Journal: BMC biotechnology (2017): 56
References
View all 49 references: Citation Explorer
Beyond click chemistry - supramolecular interactions of 1,2,3-triazoles
Authors: Schulze B, Schubert US.
Journal: Chem Soc Rev (2014): 2522
Authors: Schulze B, Schubert US.
Journal: Chem Soc Rev (2014): 2522
Calixarene-based chemosensors by means of click chemistry
Authors: Song M, Sun Z, Han C, Tian D, Li H, Kim JS.
Journal: Chem Asian J (2014): 2344
Authors: Song M, Sun Z, Han C, Tian D, Li H, Kim JS.
Journal: Chem Asian J (2014): 2344
Use of click-chemistry in the development of peptidomimetic enzyme inhibitors
Authors: Fabbrizzi P, Menchi G, Guarna A, Trabocchi A.
Journal: Curr Med Chem (2014): 1467
Authors: Fabbrizzi P, Menchi G, Guarna A, Trabocchi A.
Journal: Curr Med Chem (2014): 1467
Applications of copper-catalyzed click chemistry in activity-based protein profiling
Authors: Martell J, Weerapana E.
Journal: Molecules (2014): 1378
Authors: Martell J, Weerapana E.
Journal: Molecules (2014): 1378
'Click chemistry' for diagnosis: a patent review on exploitation of its emerging trends
Authors: M, undefined and hare A, Banerjee P, Bhutkar S, Hirwani R.
Journal: Expert Opin Ther Pat (2014): 1287
Authors: M, undefined and hare A, Banerjee P, Bhutkar S, Hirwani R.
Journal: Expert Opin Ther Pat (2014): 1287