Cy3B DBCO

Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. However, Cy3 suffers low fluorescence intensity. It is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3B DBCO can be used for conjugations with azide-containing biological molecules under copper-free conditions. Cy3® is the trademark of GE Healthcare.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Cy3B acid	560	571	120000¹	0.58¹	0.048	0.069
Cy3B alkyne	560	571	120000¹	0.58¹	0.048	0.069
Cy3B azide	560	571	120000¹	0.58¹	0.048	0.069
Cy3B maleimide	560	571	120000¹	0.58¹	0.048	0.069
Cy3B TCO	560	571	120000¹	0.58¹	0.048	0.069
Cy3B Tetrazine	560	571	120000¹	0.58¹	0.048	0.069
DBCO-Cy3	555	569	150000¹	0.15¹	0.07	0.073
DBCO-Cy5	651	670	250000¹	0.27¹, 0.4²	0.02	0.03

References

View all 16 references: Citation Explorer

Cy3-RgIA-5727 Labels and Inhibits α9-Containing nAChRs of Cochlear Hair Cells.
Authors: Fisher, Fernando and Zhang, Yuanyuan and Vincent, Philippe F Y and Gajewiak, Joanna and Gordon, Thomas J and Glowatzki, Elisabeth and Fuchs, Paul Albert and McIntosh, J Michael
Journal: Frontiers in cellular neuroscience (2021): 697560

Design, Synthesis, Conjugation, and Reactivity of Novel trans,trans-1,5-Cyclooctadiene-Derived Bioorthogonal Linkers.
Authors: Longo, Beatrice and Zanato, Chiara and Piras, Monica and Dall'Angelo, Sergio and Windhorst, Albert D and Vugts, Danielle J and Baldassarre, Massimiliano and Zanda, Matteo
Journal: Bioconjugate chemistry (2020)

Clickable Methyltetrazine-Indocarbocyanine Lipids: A Multicolor Tool Kit for Efficient Modifications of Cell Membranes.
Authors: Gaikwad, Hanmant and Wang, Guankui and Smith, Weston J and Alexander, Keisha L and D'Alessandro, Angelo and Zhang, Wei and Purev, Enkhtsetseg and Simberg, Dmitri
Journal: Bioconjugate chemistry (2019): 2106-2114

Bioorthogonally Applicable Fluorescence Deactivation Strategy for Receptor Kinetics Study and Theranostic Pretargeting Approaches.
Authors: van der Wal, Steffen and de Korne, Clarize M and Sand, Laurens G L and van Willigen, Danny M and Hogendoorn, Pancras C W and Szuhai, Karoly and van Leeuwen, Fijs W B and Buckle, Tessa
Journal: Chembiochem : a European journal of chemical biology (2018)

Modulation of Mitochondriotropic Properties of Cyanine Dyes by in Organello Copper-Free Click Reaction.
Authors: Negwer, Inka and Hirsch, Markus and Kaloyanova, Stefka and Brown, Tom and Peneva, Kalina and Butt, Hans-Jürgen and Koynov, Kaloian and Helm, Mark
Journal: Chembiochem : a European journal of chemical biology (2017): 1814-1818

Page updated on May 19, 2025

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Catalog Number	945
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Physical properties

Molecular weight	1418.70
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.048
Correction Factor (280 nm)	0.069
Extinction coefficient (cm ^-1 M ^-1)	120000¹
Excitation (nm)	560
Emission (nm)	571
Quantum yield	0.58¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501