Cy3B DBCO
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1418.70 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.048 |
Correction Factor (280 nm) | 0.069 |
Extinction coefficient (cm -1 M -1) | 1200001 |
Excitation (nm) | 560 |
Emission (nm) | 571 |
Quantum yield | 0.581 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
See also: Bioconjugation, Dye to Peptide Conjugation, Chemical Reagents, Classic Dyes, Cyanines, Peptide and Oligonucleotide Labeling
Molecular weight 1418.70 | Correction Factor (260 nm) 0.048 | Correction Factor (280 nm) 0.069 | Extinction coefficient (cm -1 M -1) 1200001 | Excitation (nm) 560 | Emission (nm) 571 | Quantum yield 0.581 |
Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. However, Cy3 suffers low fluorescence intensity. It is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3B DBCO can be used for conjugations with azide-containing biological molecules under copper-free conditions. Cy3® is the trademark of GE Healthcare.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3B DBCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 70.487 µL | 352.435 µL | 704.871 µL | 3.524 mL | 7.049 mL |
5 mM | 14.097 µL | 70.487 µL | 140.974 µL | 704.871 µL | 1.41 mL |
10 mM | 7.049 µL | 35.244 µL | 70.487 µL | 352.435 µL | 704.871 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.048 |
Correction Factor (280 nm) | 0.069 |
Extinction coefficient (cm -1 M -1) | 1200001 |
Excitation (nm) | 560 |
Emission (nm) | 571 |
Quantum yield | 0.581 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
Cy3B acid | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
Cy3B maleimide | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
Cy3B azide | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
Cy3B alkyne | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
Cy3B TCO | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
Cy3B Tetrazine | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
DBCO-Cy3 | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
DBCO-Cy5 | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
References
View all 16 references: Citation Explorer
Cy3-RgIA-5727 Labels and Inhibits α9-Containing nAChRs of Cochlear Hair Cells.
Authors: Fisher, Fernando and Zhang, Yuanyuan and Vincent, Philippe F Y and Gajewiak, Joanna and Gordon, Thomas J and Glowatzki, Elisabeth and Fuchs, Paul Albert and McIntosh, J Michael
Journal: Frontiers in cellular neuroscience (2021): 697560
Authors: Fisher, Fernando and Zhang, Yuanyuan and Vincent, Philippe F Y and Gajewiak, Joanna and Gordon, Thomas J and Glowatzki, Elisabeth and Fuchs, Paul Albert and McIntosh, J Michael
Journal: Frontiers in cellular neuroscience (2021): 697560
Design, Synthesis, Conjugation, and Reactivity of Novel trans,trans-1,5-Cyclooctadiene-Derived Bioorthogonal Linkers.
Authors: Longo, Beatrice and Zanato, Chiara and Piras, Monica and Dall'Angelo, Sergio and Windhorst, Albert D and Vugts, Danielle J and Baldassarre, Massimiliano and Zanda, Matteo
Journal: Bioconjugate chemistry (2020)
Authors: Longo, Beatrice and Zanato, Chiara and Piras, Monica and Dall'Angelo, Sergio and Windhorst, Albert D and Vugts, Danielle J and Baldassarre, Massimiliano and Zanda, Matteo
Journal: Bioconjugate chemistry (2020)
Clickable Methyltetrazine-Indocarbocyanine Lipids: A Multicolor Tool Kit for Efficient Modifications of Cell Membranes.
Authors: Gaikwad, Hanmant and Wang, Guankui and Smith, Weston J and Alexander, Keisha L and D'Alessandro, Angelo and Zhang, Wei and Purev, Enkhtsetseg and Simberg, Dmitri
Journal: Bioconjugate chemistry (2019): 2106-2114
Authors: Gaikwad, Hanmant and Wang, Guankui and Smith, Weston J and Alexander, Keisha L and D'Alessandro, Angelo and Zhang, Wei and Purev, Enkhtsetseg and Simberg, Dmitri
Journal: Bioconjugate chemistry (2019): 2106-2114
Bioorthogonally Applicable Fluorescence Deactivation Strategy for Receptor Kinetics Study and Theranostic Pretargeting Approaches.
Authors: van der Wal, Steffen and de Korne, Clarize M and Sand, Laurens G L and van Willigen, Danny M and Hogendoorn, Pancras C W and Szuhai, Karoly and van Leeuwen, Fijs W B and Buckle, Tessa
Journal: Chembiochem : a European journal of chemical biology (2018)
Authors: van der Wal, Steffen and de Korne, Clarize M and Sand, Laurens G L and van Willigen, Danny M and Hogendoorn, Pancras C W and Szuhai, Karoly and van Leeuwen, Fijs W B and Buckle, Tessa
Journal: Chembiochem : a European journal of chemical biology (2018)
Modulation of Mitochondriotropic Properties of Cyanine Dyes by in Organello Copper-Free Click Reaction.
Authors: Negwer, Inka and Hirsch, Markus and Kaloyanova, Stefka and Brown, Tom and Peneva, Kalina and Butt, Hans-Jürgen and Koynov, Kaloian and Helm, Mark
Journal: Chembiochem : a European journal of chemical biology (2017): 1814-1818
Authors: Negwer, Inka and Hirsch, Markus and Kaloyanova, Stefka and Brown, Tom and Peneva, Kalina and Butt, Hans-Jürgen and Koynov, Kaloian and Helm, Mark
Journal: Chembiochem : a European journal of chemical biology (2017): 1814-1818
FRET Characterization of Complex Conformational Changes in a Large 16S Ribosomal RNA Fragment Site-Specifically Labeled Using Unnatural Base Pairs.
Authors: Lavergne, Thomas and Lamichhane, Rajan and Malyshev, Denis A and Li, Zhengtao and Li, Lingjun and Sperling, Edit and Williamson, James R and Millar, David P and Romesberg, Floyd E
Journal: ACS chemical biology (2016): 1347-53
Authors: Lavergne, Thomas and Lamichhane, Rajan and Malyshev, Denis A and Li, Zhengtao and Li, Lingjun and Sperling, Edit and Williamson, James R and Millar, David P and Romesberg, Floyd E
Journal: ACS chemical biology (2016): 1347-53
Azide and trans-cyclooctene dUTPs: incorporation into DNA probes and fluorescent click-labelling.
Authors: Ren, Xiaomei and El-Sagheer, Afaf H and Brown, Tom
Journal: The Analyst (2015): 2671-8
Authors: Ren, Xiaomei and El-Sagheer, Afaf H and Brown, Tom
Journal: The Analyst (2015): 2671-8
Programmed Synthesis by Stimuli-Responsive DNAzyme-Modified Mesoporous SiO2 Nanoparticles.
Authors: Balogh, Dora and Aleman Garcia, Miguel Angel and Albada, H Bauke and Willner, Itamar
Journal: Angewandte Chemie (International ed. in English) (2015): 11652-6
Authors: Balogh, Dora and Aleman Garcia, Miguel Angel and Albada, H Bauke and Willner, Itamar
Journal: Angewandte Chemie (International ed. in English) (2015): 11652-6
Clickable degradable aliphatic polyesters via copolymerization with alkyne epoxy esters: synthesis and postfunctionalization with organic dyes.
Authors: Teske, Nele S and Voigt, Julia and Shastri, V Prasad
Journal: Journal of the American Chemical Society (2014): 10527-33
Authors: Teske, Nele S and Voigt, Julia and Shastri, V Prasad
Journal: Journal of the American Chemical Society (2014): 10527-33
Purification and assembly of thermostable Cy5 labeled γ-PNAs into a 3D DNA nanocage.
Authors: Flory, Justin D and Johnson, Trey and Simmons, Chad R and Lin, Su and Ghirlanda, Giovanna and Fromme, Petra
Journal: Artificial DNA, PNA & XNA (2014): 1-8
Authors: Flory, Justin D and Johnson, Trey and Simmons, Chad R and Lin, Su and Ghirlanda, Giovanna and Fromme, Petra
Journal: Artificial DNA, PNA & XNA (2014): 1-8