Cy3B DBCO

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1418.70
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.048
Correction Factor (280 nm)	0.069
Extinction coefficient (cm ^-1 M ^-1)	120000¹
Excitation (nm)	560
Emission (nm)	571
Quantum yield	0.58¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

1418.70

Correction Factor (260 nm)

0.048

Correction Factor (280 nm)

0.069

Extinction coefficient (cm ^-1 M ^-1)

120000¹

Excitation (nm)

560

Emission (nm)

571

Quantum yield

0.58¹

Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. However, Cy3 suffers low fluorescence intensity. It is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3B DBCO can be used for conjugations with azide-containing biological molecules under copper-free conditions. Cy3® is the trademark of GE Healthcare.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3B DBCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	70.487 µL	352.435 µL	704.871 µL	3.524 mL	7.049 mL
5 mM	14.097 µL	70.487 µL	140.974 µL	704.871 µL	1.41 mL
10 mM	7.049 µL	35.244 µL	70.487 µL	352.435 µL	704.871 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.048
Correction Factor (280 nm)	0.069
Extinction coefficient (cm ^-1 M ^-1)	120000¹
Excitation (nm)	560
Emission (nm)	571
Quantum yield	0.58¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Cy3B acid	560	571	120000¹	0.58¹	0.048	0.069
Cy3B maleimide	560	571	120000¹	0.58¹	0.048	0.069
Cy3B azide	560	571	120000¹	0.58¹	0.048	0.069
Cy3B alkyne	560	571	120000¹	0.58¹	0.048	0.069
Cy3B TCO	560	571	120000¹	0.58¹	0.048	0.069
Cy3B Tetrazine	560	571	120000¹	0.58¹	0.048	0.069
DBCO-Cy3	555	569	150000¹	0.15¹	0.07	0.073
DBCO-Cy5	651	670	250000¹	0.27¹, 0.4²	0.02	0.03

Images

Figure 1. Chemical structure for Cy3B DBCO.

References

View all 16 references: Citation Explorer

Cy3-RgIA-5727 Labels and Inhibits α9-Containing nAChRs of Cochlear Hair Cells.
Authors: Fisher, Fernando and Zhang, Yuanyuan and Vincent, Philippe F Y and Gajewiak, Joanna and Gordon, Thomas J and Glowatzki, Elisabeth and Fuchs, Paul Albert and McIntosh, J Michael
Journal: Frontiers in cellular neuroscience (2021): 697560

Design, Synthesis, Conjugation, and Reactivity of Novel trans,trans-1,5-Cyclooctadiene-Derived Bioorthogonal Linkers.
Authors: Longo, Beatrice and Zanato, Chiara and Piras, Monica and Dall'Angelo, Sergio and Windhorst, Albert D and Vugts, Danielle J and Baldassarre, Massimiliano and Zanda, Matteo
Journal: Bioconjugate chemistry (2020)

Clickable Methyltetrazine-Indocarbocyanine Lipids: A Multicolor Tool Kit for Efficient Modifications of Cell Membranes.
Authors: Gaikwad, Hanmant and Wang, Guankui and Smith, Weston J and Alexander, Keisha L and D'Alessandro, Angelo and Zhang, Wei and Purev, Enkhtsetseg and Simberg, Dmitri
Journal: Bioconjugate chemistry (2019): 2106-2114

Bioorthogonally Applicable Fluorescence Deactivation Strategy for Receptor Kinetics Study and Theranostic Pretargeting Approaches.
Authors: van der Wal, Steffen and de Korne, Clarize M and Sand, Laurens G L and van Willigen, Danny M and Hogendoorn, Pancras C W and Szuhai, Karoly and van Leeuwen, Fijs W B and Buckle, Tessa
Journal: Chembiochem : a European journal of chemical biology (2018)

Modulation of Mitochondriotropic Properties of Cyanine Dyes by in Organello Copper-Free Click Reaction.
Authors: Negwer, Inka and Hirsch, Markus and Kaloyanova, Stefka and Brown, Tom and Peneva, Kalina and Butt, Hans-Jürgen and Koynov, Kaloian and Helm, Mark
Journal: Chembiochem : a European journal of chemical biology (2017): 1814-1818

FRET Characterization of Complex Conformational Changes in a Large 16S Ribosomal RNA Fragment Site-Specifically Labeled Using Unnatural Base Pairs.
Authors: Lavergne, Thomas and Lamichhane, Rajan and Malyshev, Denis A and Li, Zhengtao and Li, Lingjun and Sperling, Edit and Williamson, James R and Millar, David P and Romesberg, Floyd E
Journal: ACS chemical biology (2016): 1347-53

Azide and trans-cyclooctene dUTPs: incorporation into DNA probes and fluorescent click-labelling.
Authors: Ren, Xiaomei and El-Sagheer, Afaf H and Brown, Tom
Journal: The Analyst (2015): 2671-8

Programmed Synthesis by Stimuli-Responsive DNAzyme-Modified Mesoporous SiO2 Nanoparticles.
Authors: Balogh, Dora and Aleman Garcia, Miguel Angel and Albada, H Bauke and Willner, Itamar
Journal: Angewandte Chemie (International ed. in English) (2015): 11652-6

Clickable degradable aliphatic polyesters via copolymerization with alkyne epoxy esters: synthesis and postfunctionalization with organic dyes.
Authors: Teske, Nele S and Voigt, Julia and Shastri, V Prasad
Journal: Journal of the American Chemical Society (2014): 10527-33

Purification and assembly of thermostable Cy5 labeled γ-PNAs into a 3D DNA nanocage.
Authors: Flory, Justin D and Johnson, Trey and Simmons, Chad R and Lin, Su and Ghirlanda, Giovanna and Fromme, Petra
Journal: Artificial DNA, PNA & XNA (2014): 1-8