AAT Bioquest


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Physical properties
Molecular weight1418.70
Spectral properties
Correction Factor (260 nm)0.048
Correction Factor (280 nm)0.069
Extinction coefficient (cm -1 M -1)1200001
Excitation (nm)560
Emission (nm)571
Quantum yield0.581
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Cyanines
Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. However, Cy3 suffers low fluorescence intensity. It is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3B DBCO can be used for conjugations with azide-containing biological molecules under copper-free conditions. Cy3® is the trademark of GE Healthcare.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3B DBCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM70.487 µL352.435 µL704.871 µL3.524 mL7.049 mL
5 mM14.097 µL70.487 µL140.974 µL704.871 µL1.41 mL
10 mM7.049 µL35.244 µL70.487 µL352.435 µL704.871 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectral properties

Correction Factor (260 nm)0.048
Correction Factor (280 nm)0.069
Extinction coefficient (cm -1 M -1)1200001
Excitation (nm)560
Emission (nm)571
Quantum yield0.581

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Cy3B acid56057112000010.5810.0480.069
Cy3B maleimide56057112000010.5810.0480.069
Cy3B azide56057112000010.5810.0480.069
Cy3B alkyne56057112000010.5810.0480.069
Cy3B TCO56057112000010.5810.0480.069
Cy3B Tetrazine56057112000010.5810.0480.069
DBCO-Cy565167025000010.271, 0.420.020.03



View all 16 references: Citation Explorer
Cy3-RgIA-5727 Labels and Inhibits α9-Containing nAChRs of Cochlear Hair Cells.
Authors: Fisher, Fernando and Zhang, Yuanyuan and Vincent, Philippe F Y and Gajewiak, Joanna and Gordon, Thomas J and Glowatzki, Elisabeth and Fuchs, Paul Albert and McIntosh, J Michael
Journal: Frontiers in cellular neuroscience (2021): 697560
Design, Synthesis, Conjugation, and Reactivity of Novel trans,trans-1,5-Cyclooctadiene-Derived Bioorthogonal Linkers.
Authors: Longo, Beatrice and Zanato, Chiara and Piras, Monica and Dall'Angelo, Sergio and Windhorst, Albert D and Vugts, Danielle J and Baldassarre, Massimiliano and Zanda, Matteo
Journal: Bioconjugate chemistry (2020)
Clickable Methyltetrazine-Indocarbocyanine Lipids: A Multicolor Tool Kit for Efficient Modifications of Cell Membranes.
Authors: Gaikwad, Hanmant and Wang, Guankui and Smith, Weston J and Alexander, Keisha L and D'Alessandro, Angelo and Zhang, Wei and Purev, Enkhtsetseg and Simberg, Dmitri
Journal: Bioconjugate chemistry (2019): 2106-2114
Bioorthogonally Applicable Fluorescence Deactivation Strategy for Receptor Kinetics Study and Theranostic Pretargeting Approaches.
Authors: van der Wal, Steffen and de Korne, Clarize M and Sand, Laurens G L and van Willigen, Danny M and Hogendoorn, Pancras C W and Szuhai, Karoly and van Leeuwen, Fijs W B and Buckle, Tessa
Journal: Chembiochem : a European journal of chemical biology (2018)
Modulation of Mitochondriotropic Properties of Cyanine Dyes by in Organello Copper-Free Click Reaction.
Authors: Negwer, Inka and Hirsch, Markus and Kaloyanova, Stefka and Brown, Tom and Peneva, Kalina and Butt, Hans-Jürgen and Koynov, Kaloian and Helm, Mark
Journal: Chembiochem : a European journal of chemical biology (2017): 1814-1818
FRET Characterization of Complex Conformational Changes in a Large 16S Ribosomal RNA Fragment Site-Specifically Labeled Using Unnatural Base Pairs.
Authors: Lavergne, Thomas and Lamichhane, Rajan and Malyshev, Denis A and Li, Zhengtao and Li, Lingjun and Sperling, Edit and Williamson, James R and Millar, David P and Romesberg, Floyd E
Journal: ACS chemical biology (2016): 1347-53
Programmed Synthesis by Stimuli-Responsive DNAzyme-Modified Mesoporous SiO2 Nanoparticles.
Authors: Balogh, Dora and Aleman Garcia, Miguel Angel and Albada, H Bauke and Willner, Itamar
Journal: Angewandte Chemie (International ed. in English) (2015): 11652-6
Azide and trans-cyclooctene dUTPs: incorporation into DNA probes and fluorescent click-labelling.
Authors: Ren, Xiaomei and El-Sagheer, Afaf H and Brown, Tom
Journal: The Analyst (2015): 2671-8
Purification and assembly of thermostable Cy5 labeled γ-PNAs into a 3D DNA nanocage.
Authors: Flory, Justin D and Johnson, Trey and Simmons, Chad D and Lin, Su and Ghirlanda, Giovanna and Fromme, Petra
Journal: Artificial DNA, PNA & XNA (2014): e992181
Enzymatic incorporation and fluorescent labelling of cyclooctyne-modified deoxyuridine triphosphates in DNA.
Authors: Ren, Xiaomei and Gerowska, Marta and El-Sagheer, Afaf H and Brown, Tom
Journal: Bioorganic & medicinal chemistry (2014): 4384-90