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Cy3B TCO
Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. However, Cy3 suffers low fluorescence intensity. It is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3B TCO can be used for conjugations with tetrazine-containing biological molecules under copper-free conditions. Compared to the other click reactions, the TCO-tetrazine reaction gives much higher yields under mild reaction conditions. Cy3® is the trademark of GE Healthcare.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Cy3B acid | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
| Cy3B alkyne | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
| Cy3B azide | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
| Cy3B DBCO | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
| Cy3B maleimide | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
| Cy3B Tetrazine | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
References
View all 3 references: Citation Explorer
Design, Synthesis, Conjugation, and Reactivity of Novel trans,trans-1,5-Cyclooctadiene-Derived Bioorthogonal Linkers.
Authors: Longo, Beatrice and Zanato, Chiara and Piras, Monica and Dall'Angelo, Sergio and Windhorst, Albert D and Vugts, Danielle J and Baldassarre, Massimiliano and Zanda, Matteo
Journal: Bioconjugate chemistry (2020)
Authors: Longo, Beatrice and Zanato, Chiara and Piras, Monica and Dall'Angelo, Sergio and Windhorst, Albert D and Vugts, Danielle J and Baldassarre, Massimiliano and Zanda, Matteo
Journal: Bioconjugate chemistry (2020)
Clickable Methyltetrazine-Indocarbocyanine Lipids: A Multicolor Tool Kit for Efficient Modifications of Cell Membranes.
Authors: Gaikwad, Hanmant and Wang, Guankui and Smith, Weston J and Alexander, Keisha L and D'Alessandro, Angelo and Zhang, Wei and Purev, Enkhtsetseg and Simberg, Dmitri
Journal: Bioconjugate chemistry (2019): 2106-2114
Authors: Gaikwad, Hanmant and Wang, Guankui and Smith, Weston J and Alexander, Keisha L and D'Alessandro, Angelo and Zhang, Wei and Purev, Enkhtsetseg and Simberg, Dmitri
Journal: Bioconjugate chemistry (2019): 2106-2114
Azide and trans-cyclooctene dUTPs: incorporation into DNA probes and fluorescent click-labelling.
Authors: Ren, Xiaomei and El-Sagheer, Afaf H and Brown, Tom
Journal: The Analyst (2015): 2671-8
Authors: Ren, Xiaomei and El-Sagheer, Afaf H and Brown, Tom
Journal: The Analyst (2015): 2671-8
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