Cy3B TCO
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1093.34 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.048 |
| Correction Factor (280 nm) | 0.069 |
| Extinction coefficient (cm -1 M -1) | 1200001 |
| Excitation (nm) | 560 |
| Emission (nm) | 571 |
| Quantum yield | 0.581 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Direct upgrades
| iFluor® 555 TCO |
Related products
| Overview |  SDSProtocol |
Upgrade: iFluor® 555 TCO
See also: Cyanines
Molecular weight 1093.34 | Correction Factor (260 nm) 0.048 | Correction Factor (280 nm) 0.069 | Extinction coefficient (cm -1 M -1) 1200001 | Excitation (nm) 560 | Emission (nm) 571 | Quantum yield 0.581 |
Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. However, Cy3 suffers low fluorescence intensity. It is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3B TCO can be used for conjugations with tetrazine-containing biological molecules under copper-free conditions. Compared to the other click reactions, the TCO-tetrazine reaction gives much higher yields under mild reaction conditions. Cy3® is the trademark of GE Healthcare.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3B TCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 91.463 µL | 457.314 µL | 914.629 µL | 4.573 mL | 9.146 mL |
| 5 mM | 18.293 µL | 91.463 µL | 182.926 µL | 914.629 µL | 1.829 mL |
| 10 mM | 9.146 µL | 45.731 µL | 91.463 µL | 457.314 µL | 914.629 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.048 |
| Correction Factor (280 nm) | 0.069 |
| Extinction coefficient (cm -1 M -1) | 1200001 |
| Excitation (nm) | 560 |
| Emission (nm) | 571 |
| Quantum yield | 0.581 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Cy3B acid | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
| Cy3B maleimide | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
| Cy3B azide | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
| Cy3B alkyne | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
| Cy3B DBCO | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
| Cy3B Tetrazine | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
| XFD488 TCO | 499 | 520 | 73000 | 0.921 | 0.3 | 0.11 |
Images
Figure 1. Chemical structure for Cy3B TCO.
References
View all 3 references: Citation Explorer
Design, Synthesis, Conjugation, and Reactivity of Novel trans,trans-1,5-Cyclooctadiene-Derived Bioorthogonal Linkers.
Authors: Longo, Beatrice and Zanato, Chiara and Piras, Monica and Dall'Angelo, Sergio and Windhorst, Albert D and Vugts, Danielle J and Baldassarre, Massimiliano and Zanda, Matteo
Journal: Bioconjugate chemistry (2020)
Authors: Longo, Beatrice and Zanato, Chiara and Piras, Monica and Dall'Angelo, Sergio and Windhorst, Albert D and Vugts, Danielle J and Baldassarre, Massimiliano and Zanda, Matteo
Journal: Bioconjugate chemistry (2020)
Clickable Methyltetrazine-Indocarbocyanine Lipids: A Multicolor Tool Kit for Efficient Modifications of Cell Membranes.
Authors: Gaikwad, Hanmant and Wang, Guankui and Smith, Weston J and Alexander, Keisha L and D'Alessandro, Angelo and Zhang, Wei and Purev, Enkhtsetseg and Simberg, Dmitri
Journal: Bioconjugate chemistry (2019): 2106-2114
Authors: Gaikwad, Hanmant and Wang, Guankui and Smith, Weston J and Alexander, Keisha L and D'Alessandro, Angelo and Zhang, Wei and Purev, Enkhtsetseg and Simberg, Dmitri
Journal: Bioconjugate chemistry (2019): 2106-2114
Azide and trans-cyclooctene dUTPs: incorporation into DNA probes and fluorescent click-labelling.
Authors: Ren, Xiaomei and El-Sagheer, Afaf H and Brown, Tom
Journal: The Analyst (2015): 2671-8
Authors: Ren, Xiaomei and El-Sagheer, Afaf H and Brown, Tom
Journal: The Analyst (2015): 2671-8