AAT Bioquest

Cy3B acid

Product Image
Product Image
Gallery Image 1
Ordering information
Catalog Number
Unit Size
Add to cart
Additional ordering information
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight560.67
Spectral properties
Correction Factor (260 nm)0.048
Correction Factor (280 nm)0.069
Extinction coefficient (cm -1 M -1)1200001
Excitation (nm)560
Emission (nm)571
Quantum yield0.581
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure


See also: Cyanines
Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy3 is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3® is the trademark of GE Healthcare.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3B acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM178.358 µL891.79 µL1.784 mL8.918 mL17.836 mL
5 mM35.672 µL178.358 µL356.716 µL1.784 mL3.567 mL
10 mM17.836 µL89.179 µL178.358 µL891.79 µL1.784 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles


Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)0.048
Correction Factor (280 nm)0.069
Extinction coefficient (cm -1 M -1)1200001
Excitation (nm)560
Emission (nm)571
Quantum yield0.581

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Cy3NS acid *CAS 1032678-01-5*55556915000010.1510.070.073
Cy3B DBCO56057112000010.5810.0480.069
Cy3B TCO56057112000010.5810.0480.069
Cy3B Tetrazine56057112000010.5810.0480.069
ICG acid789813230000--0.076
Cy5NS acid65167025000010.271, 0.420.020.03
Cy7NS acid7567792500000.30.050.036
AMCA acid *CAS#: 106562-32-7*34643419000--0.153
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] *CAS 50402-56-7*3364555900--0.107
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]3364555900--0.107
DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1*-----0.516
BCECF acid *CAS#: 85138-49-4*50452790000---
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid*534553810000.6110.240.09
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid*499520710000.9210.300.11
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid*34344119000-0.250.19
XFD546 acid *Same Structure to Alexa Fluor™ 546 acid*5615721120000.7910.210.12
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid*579603913000.6910.450.46
XFD514 acid51854380000-0.310.18
QXY7 acid [equivalent to QSY-7 acid]--900001--0.22
XFD555 acid5535681550000.110.080.08
XFD647 acid6506712700000.331-0.03
XFD750 acid7527762900000.121-0.04
XFD700 acid6967192050000.251-0.07
OG514 acid [equivalent to Oregon Green® 514 carboxylic acid]51353385000--0.19
Show More (15)



View all 3 citations: Citation Explorer
Homogeneous fluorescence anisotropy-based assay for characterization of ligand binding dynamics to GPCRs in budded baculoviruses: the case of Cy3B-NDP-alpha-MSH binding to MC4 receptors
Authors: Veiksina, S., Kopanchuk, S., Mazina, O., Link, R., Lille, A., Rinken, A.
Journal: Methods Mol Biol (2015): 37-50
A highly fluorescent DNA toolkit: synthesis and properties of oligonucleotides containing new Cy3, Cy5 and Cy3B monomers
Authors: Hall, L. M., Gerowska, M., Brown, T.
Journal: Nucleic Acids Res (2012): e108
Cy3B: improving the performance of cyanine dyes
Authors: Cooper, M., Ebner, A., Briggs, M., Burrows, M., Gardner, N., Richardson, R., West, R.
Journal: J Fluoresc (2004): 145-50