Cy3B acid | AAT Bioquest

Cy3B acid

Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy3 is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3® is the trademark of GE Healthcare.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
940	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3B acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	178.358 µL	891.79 µL	1.784 mL	8.918 mL	17.836 mL
5 mM	35.672 µL	178.358 µL	356.716 µL	1.784 mL	3.567 mL
10 mM	17.836 µL	89.179 µL	178.358 µL	891.79 µL	1.784 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	560.67 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Homogeneous fluorescence anisotropy-based assay for characterization of ligand binding dynamics to GPCRs in budded baculoviruses: the case of Cy3B-NDP-alpha-MSH binding to MC4 receptors

Authors:

Veiksina, S., Kopanchuk, S., Mazina, O., Link, R., Lille, A., Rinken, A.

Journal:

Methods Mol Biol (2015): 37-50

A highly fluorescent DNA toolkit: synthesis and properties of oligonucleotides containing new Cy3, Cy5 and Cy3B monomers

Authors:

Hall, L. M., Gerowska, M., Brown, T.

Journal:

Nucleic Acids Res (2012): e108

Cy3B: improving the performance of cyanine dyes

Authors:

Cooper, M., Ebner, A., Briggs, M., Burrows, M., Gardner, N., Richardson, R., West, R.

Journal:

J Fluoresc (2004): 145-50

Physical properties

Molecular weight	560.67
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.048
Correction factor (280 nm)	0.069
Extinction coefficient (cm ^-1 M ^-1)	120000 ¹
Excitation (nm)	560
Emission (nm)	571
Quantum yield	0.58 ¹

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on September 27, 2025