Cy3B Tetrazine
Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. However, Cy3 suffers low fluorescence intensity. It is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3B tetrazine can be used for conjugations with TCO-containing biological molecules under copper-free conditions. Compared to the other click reactions, the TCO-tetrazine reaction gives much higher yields under mild reaction conditions. Cy3® is the trademark of GE Healthcare.
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
Cy3 tetrazine | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
Cy3B acid | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
Cy3B maleimide | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
Cy3B azide | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
Cy3B alkyne | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
Cy3B DBCO | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
Cy3B TCO | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
Cy5 tetrazine | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
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Citations
View all 1 citations: Citation Explorer
Structural basis of orientated asymmetry in a mGlu heterodimer
Authors: Huang, Weizhu and Jin, Nan and Guo, Jia and Shen, Cangsong and Xu, Chanjuan and Xi, Kun and Bonhomme, L{\'e}o and Quast, Robert B and Shen, Dan-Dan and Qin, Jiao and others,
Journal: Nature Communications (2024): 1--15
Authors: Huang, Weizhu and Jin, Nan and Guo, Jia and Shen, Cangsong and Xu, Chanjuan and Xi, Kun and Bonhomme, L{\'e}o and Quast, Robert B and Shen, Dan-Dan and Qin, Jiao and others,
Journal: Nature Communications (2024): 1--15
References
View all 37 references: Citation Explorer
Fluorescence Anisotropy-Based Assay for Characterization of Ligand Binding Dynamics to GPCRs: The Case of Cy3B-Labeled Ligands Binding to MC4 Receptors in Budded Baculoviruses.
Authors: Veiksina, Santa and Tahk, Maris-Johanna and Laasfeld, Tõnis and Link, Reet and Kopanchuk, Sergei and Rinken, Ago
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 119-136
Authors: Veiksina, Santa and Tahk, Maris-Johanna and Laasfeld, Tõnis and Link, Reet and Kopanchuk, Sergei and Rinken, Ago
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 119-136
Quantitative analysis of fluorescent ligand binding to dopamine D3 receptors using live-cell microscopy.
Authors: Allikalt, Anni and Laasfeld, Tõnis and Ilisson, Mihkel and Kopanchuk, Sergei and Rinken, Ago
Journal: The FEBS journal (2021): 1514-1532
Authors: Allikalt, Anni and Laasfeld, Tõnis and Ilisson, Mihkel and Kopanchuk, Sergei and Rinken, Ago
Journal: The FEBS journal (2021): 1514-1532
Fluorescent ligands for dopamine D2/D3 receptors.
Authors: Allikalt, Anni and Purkayastha, Nirupam and Flad, Khajidmaa and Schmidt, Maximilian F and Tabor, Alina and Gmeiner, Peter and Hübner, Harald and Weikert, Dorothee
Journal: Scientific reports (2020): 21842
Authors: Allikalt, Anni and Purkayastha, Nirupam and Flad, Khajidmaa and Schmidt, Maximilian F and Tabor, Alina and Gmeiner, Peter and Hübner, Harald and Weikert, Dorothee
Journal: Scientific reports (2020): 21842
Unifying Mechanism for Thiol-Induced Photoswitching and Photostability of Cyanine Dyes.
Authors: Gidi, Yasser and Payne, Liam and Glembockyte, Viktorija and Michie, Megan S and Schnermann, Martin J and Cosa, Gonzalo
Journal: Journal of the American Chemical Society (2020): 12681-12689
Authors: Gidi, Yasser and Payne, Liam and Glembockyte, Viktorija and Michie, Megan S and Schnermann, Martin J and Cosa, Gonzalo
Journal: Journal of the American Chemical Society (2020): 12681-12689
Fluorescence lifetime imaging with a single-photon SPAD array using long overlapping gates: an experimental and theoretical study.
Authors: Ardelean, Andrei and Ulku, Arin Can and Michalet, Xavier and Charbon, Edoardo and Bruschini, Claudio
Journal: Proceedings of SPIE--the International Society for Optical Engineering (2019)
Authors: Ardelean, Andrei and Ulku, Arin Can and Michalet, Xavier and Charbon, Edoardo and Bruschini, Claudio
Journal: Proceedings of SPIE--the International Society for Optical Engineering (2019)
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