logo
AAT Bioquest

Cy3B Tetrazine

Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. However, Cy3 suffers low fluorescence intensity. It is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3B tetrazine can be used for conjugations with TCO-containing biological molecules under copper-free conditions. Compared to the other click reactions, the TCO-tetrazine reaction gives much higher yields under mild reaction conditions. Cy3® is the trademark of GE Healthcare.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Cy3 tetrazine55556915000010.1510.070.073
Cy3B acid56057112000010.5810.0480.069
Cy3B maleimide56057112000010.5810.0480.069
Cy3B azide56057112000010.5810.0480.069
Cy3B alkyne56057112000010.5810.0480.069
Cy3B DBCO56057112000010.5810.0480.069
Cy3B TCO56057112000010.5810.0480.069
Cy5 tetrazine65167025000010.271, 0.420.020.03
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine*499520710000.9210.300.11
Cy7 tetrazine7567792500000.30.050.036
XFD350 tetrazine34344119000-0.250.19
XFD405 Tetrazine40142135,000-0.230.70
XFD430 Tetrazine43254015,000--0.28
XFD514 Tetrazine51854380000-0.310.18
XFD546 Tetrazine5615721120000.7910.210.12
XFD532 Tetrazine534553810000.6110.240.09
XFD568 Tetrazine579603913000.6910.450.46
XFD594 Tetrazine590618900000.6610.430.56
XFD610 tetrazine611629144,000-0.430.44
Show More (10)

Citations

View all 1 citations: Citation Explorer
Structural basis of orientated asymmetry in a mGlu heterodimer
Authors: Huang, Weizhu and Jin, Nan and Guo, Jia and Shen, Cangsong and Xu, Chanjuan and Xi, Kun and Bonhomme, L{\'e}o and Quast, Robert B and Shen, Dan-Dan and Qin, Jiao and others,
Journal: Nature Communications (2024): 1--15

References

View all 37 references: Citation Explorer
Fluorescence Anisotropy-Based Assay for Characterization of Ligand Binding Dynamics to GPCRs: The Case of Cy3B-Labeled Ligands Binding to MC4 Receptors in Budded Baculoviruses.
Authors: Veiksina, Santa and Tahk, Maris-Johanna and Laasfeld, Tõnis and Link, Reet and Kopanchuk, Sergei and Rinken, Ago
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 119-136
Quantitative analysis of fluorescent ligand binding to dopamine D3 receptors using live-cell microscopy.
Authors: Allikalt, Anni and Laasfeld, Tõnis and Ilisson, Mihkel and Kopanchuk, Sergei and Rinken, Ago
Journal: The FEBS journal (2021): 1514-1532
Fluorescent ligands for dopamine D2/D3 receptors.
Authors: Allikalt, Anni and Purkayastha, Nirupam and Flad, Khajidmaa and Schmidt, Maximilian F and Tabor, Alina and Gmeiner, Peter and Hübner, Harald and Weikert, Dorothee
Journal: Scientific reports (2020): 21842
Unifying Mechanism for Thiol-Induced Photoswitching and Photostability of Cyanine Dyes.
Authors: Gidi, Yasser and Payne, Liam and Glembockyte, Viktorija and Michie, Megan S and Schnermann, Martin J and Cosa, Gonzalo
Journal: Journal of the American Chemical Society (2020): 12681-12689
Fluorescence lifetime imaging with a single-photon SPAD array using long overlapping gates: an experimental and theoretical study.
Authors: Ardelean, Andrei and Ulku, Arin Can and Michalet, Xavier and Charbon, Edoardo and Bruschini, Claudio
Journal: Proceedings of SPIE--the International Society for Optical Engineering (2019)
Page updated on December 6, 2024

Ordering information

Price
Unit size
Catalog Number948
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

729.86

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.048

Correction Factor (280 nm)

0.069

Extinction coefficient (cm -1 M -1)

1200001

Excitation (nm)

560

Emission (nm)

571

Quantum yield

0.581

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Product Image
Product Image
Gallery Image 1

Direct upgrades