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Cy3B Tetrazine

Chemical structure for Cy3B Tetrazine.
Chemical structure for Cy3B Tetrazine.
Ordering information
Price ()
Catalog Number948
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.048
Correction Factor (280 nm)0.069
Extinction coefficient (cm -1 M -1)1200001
Excitation (nm)560
Emission (nm)571
Quantum yield0.581
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Direct upgrades
iFluor® 555 Tetrazine

OverviewpdfSDS


Correction Factor (260 nm)
0.048
Correction Factor (280 nm)
0.069
Extinction coefficient (cm -1 M -1)
1200001
Excitation (nm)
560
Emission (nm)
571
Quantum yield
0.581
Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. However, Cy3 suffers low fluorescence intensity. It is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3B tetrazine can be used for conjugations with TCO-containing biological molecules under copper-free conditions. Compared to the other click reactions, the TCO-tetrazine reaction gives much higher yields under mild reaction conditions. Cy3® is the trademark of GE Healthcare.

Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.048
Correction Factor (280 nm)0.069
Extinction coefficient (cm -1 M -1)1200001
Excitation (nm)560
Emission (nm)571
Quantum yield0.581

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Cy3 tetrazine55556915000010.1510.070.073
Cy3B acid56057112000010.5810.0480.069
Cy3B maleimide56057112000010.5810.0480.069
Cy3B azide56057112000010.5810.0480.069
Cy3B alkyne56057112000010.5810.0480.069
Cy3B DBCO56057112000010.5810.0480.069
Cy3B TCO56057112000010.5810.0480.069
Cy5 tetrazine65167025000010.271, 0.420.020.03
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine*499520730000.921--

References


View all 37 references: Citation Explorer
Fluorescence Anisotropy-Based Assay for Characterization of Ligand Binding Dynamics to GPCRs: The Case of Cy3B-Labeled Ligands Binding to MC4 Receptors in Budded Baculoviruses.
Authors: Veiksina, Santa and Tahk, Maris-Johanna and Laasfeld, Tõnis and Link, Reet and Kopanchuk, Sergei and Rinken, Ago
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 119-136
Quantitative analysis of fluorescent ligand binding to dopamine D3 receptors using live-cell microscopy.
Authors: Allikalt, Anni and Laasfeld, Tõnis and Ilisson, Mihkel and Kopanchuk, Sergei and Rinken, Ago
Journal: The FEBS journal (2021): 1514-1532
Fluorescent ligands for dopamine D2/D3 receptors.
Authors: Allikalt, Anni and Purkayastha, Nirupam and Flad, Khajidmaa and Schmidt, Maximilian F and Tabor, Alina and Gmeiner, Peter and Hübner, Harald and Weikert, Dorothee
Journal: Scientific reports (2020): 21842
Unifying Mechanism for Thiol-Induced Photoswitching and Photostability of Cyanine Dyes.
Authors: Gidi, Yasser and Payne, Liam and Glembockyte, Viktorija and Michie, Megan S and Schnermann, Martin J and Cosa, Gonzalo
Journal: Journal of the American Chemical Society (2020): 12681-12689
Fluorescence lifetime imaging with a single-photon SPAD array using long overlapping gates: an experimental and theoretical study.
Authors: Ardelean, Andrei and Ulku, Arin Can and Michalet, Xavier and Charbon, Edoardo and Bruschini, Claudio
Journal: Proceedings of SPIE--the International Society for Optical Engineering (2019)
Redox-Based Photostabilizing Agents in Fluorescence Imaging: The Hidden Role of Intersystem Crossing in Geminate Radical Ion Pairs.
Authors: Glembockyte, Viktorija and Cosa, Gonzalo
Journal: Journal of the American Chemical Society (2017): 13227-13233
Characterization of ligand binding to melanocortin 4 receptors using fluorescent peptides with improved kinetic properties.
Authors: Link, Reet and Veiksina, Santa and Rinken, Ago and Kopanchuk, Sergei
Journal: European journal of pharmacology (2017): 58-66
Predicting signatures of anisotropic resonance energy transfer in dye-functionalized nanoparticles.
Authors: Gil, Gabriel and Corni, Stefano and Delgado, Alain and Bertoni, Andrea and Goldoni, Guido
Journal: RSC advances (2016): 104648-104656
Homogeneous fluorescence anisotropy-based assay for characterization of ligand binding dynamics to GPCRs in budded baculoviruses: the case of Cy3B-NDP-α-MSH binding to MC4 receptors.
Authors: Veiksina, Santa and Kopanchuk, Sergei and Mazina, Olga and Link, Reet and Lille, Anne and Rinken, Ago
Journal: Methods in molecular biology (Clifton, N.J.) (2015): 37-50
Cholesterol-induced lipophobic interaction between transmembrane helices using ensemble and single-molecule fluorescence resonance energy transfer.
Authors: Yano, Yoshiaki and Kondo, Kotaro and Kitani, Ryota and Yamamoto, Arisa and Matsuzaki, Katsumi
Journal: Biochemistry (2015): 1371-9