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Cy3B maleimide

Chemical structure for Cy3B maleimide.
Chemical structure for Cy3B maleimide.
Ordering information
Price ()
Catalog Number942
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight682.79
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.048
Correction Factor (280 nm)0.069
Extinction coefficient (cm -1 M -1)1200001
Excitation (nm)560
Emission (nm)571
Quantum yield0.581
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure
Direct upgrades
iFluor® 555 maleimide

OverviewpdfSDSpdfProtocol


Molecular weight
682.79
Correction Factor (260 nm)
0.048
Correction Factor (280 nm)
0.069
Extinction coefficient (cm -1 M -1)
1200001
Excitation (nm)
560
Emission (nm)
571
Quantum yield
0.581
Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy3 is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3® is the trademark of GE Healthcare.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3B maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM146.458 µL732.29 µL1.465 mL7.323 mL14.646 mL
5 mM29.292 µL146.458 µL292.916 µL1.465 mL2.929 mL
10 mM14.646 µL73.229 µL146.458 µL732.29 µL1.465 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.048
Correction Factor (280 nm)0.069
Extinction coefficient (cm -1 M -1)1200001
Excitation (nm)560
Emission (nm)571
Quantum yield0.581

Citations


View all 3 citations: Citation Explorer
Homogeneous fluorescence anisotropy-based assay for characterization of ligand binding dynamics to GPCRs in budded baculoviruses: the case of Cy3B-NDP-alpha-MSH binding to MC4 receptors
Authors: Veiksina, S., Kopanchuk, S., Mazina, O., Link, R., Lille, A., Rinken, A.
Journal: Methods Mol Biol (2015): 37-50
A highly fluorescent DNA toolkit: synthesis and properties of oligonucleotides containing new Cy3, Cy5 and Cy3B monomers
Authors: Hall, L. M., Gerowska, M., Brown, T.
Journal: Nucleic Acids Res (2012): e108
Cy3B: improving the performance of cyanine dyes
Authors: Cooper, M., Ebner, A., Briggs, M., Burrows, M., Gardner, N., Richardson, R., West, R.
Journal: J Fluoresc (2004): 145-50