Cy3B alkyne

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	950.15
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.048
Correction Factor (280 nm)	0.069
Extinction coefficient (cm ^-1 M ^-1)	120000¹
Excitation (nm)	560
Emission (nm)	571
Quantum yield	0.58¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Direct upgrades

Alternative formats

Overview SDS Protocol

Upgrade: iFluor® 555 alkyne

Molecular weight

950.15

Correction Factor (260 nm)

0.048

Correction Factor (280 nm)

0.069

Extinction coefficient (cm ^-1 M ^-1)

120000¹

Excitation (nm)

560

Emission (nm)

571

Quantum yield

0.58¹

Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. However, Cy3 suffers low fluorescence intensity. It is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3B alkyne can be used for conjugations with azide-containing biological molecules. Cy3® is the trademark of GE Healthcare.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3B alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	105.247 µL	526.233 µL	1.052 mL	5.262 mL	10.525 mL
5 mM	21.049 µL	105.247 µL	210.493 µL	1.052 mL	2.105 mL
10 mM	10.525 µL	52.623 µL	105.247 µL	526.233 µL	1.052 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.048
Correction Factor (280 nm)	0.069
Extinction coefficient (cm ^-1 M ^-1)	120000¹
Excitation (nm)	560
Emission (nm)	571
Quantum yield	0.58¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
Cy3B DBCO	560	571	120000¹	0.069
Cy3B TCO	560	571	120000¹	0.069
Cy3B Tetrazine	560	571	120000¹	0.069
AMCA Alkyne	346	434	19000	0.153
XFD488 alkyne Same Structure to Alexa Fluor™ 488 alkyne	490	520	71000	0.11
ICG alkyne	789	814	-	0.076
XFD647 Alkyne	650	671	270000	0.03

Images

Figure 1. Chemical structure for Cy3B alkyne.

References

View all 7 references: Citation Explorer

Sequence-Specific Post-Synthetic Oligonucleotide Labeling for Single-Molecule Fluorescence Applications.
Authors: Egloff, David and Oleinich, Igor A and Zhao, Meng and König, Sebastian L B and Sigel, Roland K O and Freisinger, Eva
Journal: ACS chemical biology (2016): 2558-67

Synthesis and labeling of α-(2,9)-trisialic acid with cyanine dyes for imaging of glycan-binding receptors on living cells.
Authors: Zhang, Xiao-tai and Gu, Zhen-yuan and Liu, Libing and Wang, Shu and Xing, Guo-wen
Journal: Chemical communications (Cambridge, England) (2015): 8606-9

A combination of metabolic labeling and 2D-DIGE analysis in response to a farnesyltransferase inhibitor facilitates the discovery of new prenylated proteins.
Authors: Palsuledesai, Charuta C and Ochocki, Joshua D and Markowski, Todd W and Distefano, Mark D
Journal: Molecular bioSystems (2014): 1094-103

Clickable degradable aliphatic polyesters via copolymerization with alkyne epoxy esters: synthesis and postfunctionalization with organic dyes.
Authors: Teske, Nele S and Voigt, Julia and Shastri, V Prasad
Journal: Journal of the American Chemical Society (2014): 10527-33

Rapid labeling of metabolically engineered cell-surface glycoconjugates with a carbamate-linked cyclopropene reporter.
Authors: Späte, Anne-Katrin and Bußkamp, Holger and Niederwieser, Andrea and Schart, Verena F and Marx, Andreas and Wittmann, Valentin
Journal: Bioconjugate chemistry (2014): 147-54

Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation.
Authors: Burnham-Marusich, Amanda R and Plechaty, Anna M and Berninsone, Patricia M
Journal: Electrophoresis (2014): 2621-5

Stepwise orthogonal click chemistry toward fabrication of paclitaxel/galactose functionalized fluorescent nanoparticles for HepG2 cell targeting and delivery.
Authors: Lai, Chian-Hui and Chang, Tsung-Che and Chuang, Yung-Jen and Tzou, Der-Lii and Lin, Chun-Cheng
Journal: Bioconjugate chemistry (2013): 1698-709