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Cy3B alkyne
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 950.15 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.048 |
| Correction Factor (280 nm) | 0.069 |
| Extinction coefficient (cm -1 M -1) | 1200001 |
| Excitation (nm) | 560 |
| Emission (nm) | 571 |
| Quantum yield | 0.581 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Direct upgrades
| iFluor® 555 alkyne |
Alternative formats
| Cy3B maleimide |
| Cy3B acid |
| Cy3B azide |
Related products
| Overview |  SDSProtocol |
Upgrade: iFluor® 555 alkyne
See also: Cyanines, Click Chemistry
Molecular weight 950.15 | Correction Factor (260 nm) 0.048 | Correction Factor (280 nm) 0.069 | Extinction coefficient (cm -1 M -1) 1200001 | Excitation (nm) 560 | Emission (nm) 571 | Quantum yield 0.581 |
Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. However, Cy3 suffers low fluorescence intensity. It is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3B alkyne can be used for conjugations with azide-containing biological molecules. Cy3® is the trademark of GE Healthcare.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3B alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 105.247 µL | 526.233 µL | 1.052 mL | 5.262 mL | 10.525 mL |
| 5 mM | 21.049 µL | 105.247 µL | 210.493 µL | 1.052 mL | 2.105 mL |
| 10 mM | 10.525 µL | 52.623 µL | 105.247 µL | 526.233 µL | 1.052 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.048 |
| Correction Factor (280 nm) | 0.069 |
| Extinction coefficient (cm -1 M -1) | 1200001 |
| Excitation (nm) | 560 |
| Emission (nm) | 571 |
| Quantum yield | 0.581 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| Cy3B DBCO | 560 | 571 | 1200001 | 0.069 |
| Cy3B TCO | 560 | 571 | 1200001 | 0.069 |
| Cy3B Tetrazine | 560 | 571 | 1200001 | 0.069 |
| AMCA Alkyne | 346 | 434 | 19000 | 0.153 |
| XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne* | 499 | 520 | 71000 | 0.11 |
| ICG alkyne | 789 | 813 | 230000 | 0.076 |
| XFD647 Alkyne | 650 | 671 | 270000 | 0.03 |
Images
Figure 1. Chemical structure for Cy3B alkyne.
References
View all 7 references: Citation Explorer
Sequence-Specific Post-Synthetic Oligonucleotide Labeling for Single-Molecule Fluorescence Applications.
Authors: Egloff, David and Oleinich, Igor A and Zhao, Meng and König, Sebastian L B and Sigel, Roland K O and Freisinger, Eva
Journal: ACS chemical biology (2016): 2558-67
Authors: Egloff, David and Oleinich, Igor A and Zhao, Meng and König, Sebastian L B and Sigel, Roland K O and Freisinger, Eva
Journal: ACS chemical biology (2016): 2558-67
Synthesis and labeling of α-(2,9)-trisialic acid with cyanine dyes for imaging of glycan-binding receptors on living cells.
Authors: Zhang, Xiao-tai and Gu, Zhen-yuan and Liu, Libing and Wang, Shu and Xing, Guo-wen
Journal: Chemical communications (Cambridge, England) (2015): 8606-9
Authors: Zhang, Xiao-tai and Gu, Zhen-yuan and Liu, Libing and Wang, Shu and Xing, Guo-wen
Journal: Chemical communications (Cambridge, England) (2015): 8606-9
A combination of metabolic labeling and 2D-DIGE analysis in response to a farnesyltransferase inhibitor facilitates the discovery of new prenylated proteins.
Authors: Palsuledesai, Charuta C and Ochocki, Joshua D and Markowski, Todd W and Distefano, Mark D
Journal: Molecular bioSystems (2014): 1094-103
Authors: Palsuledesai, Charuta C and Ochocki, Joshua D and Markowski, Todd W and Distefano, Mark D
Journal: Molecular bioSystems (2014): 1094-103
Clickable degradable aliphatic polyesters via copolymerization with alkyne epoxy esters: synthesis and postfunctionalization with organic dyes.
Authors: Teske, Nele S and Voigt, Julia and Shastri, V Prasad
Journal: Journal of the American Chemical Society (2014): 10527-33
Authors: Teske, Nele S and Voigt, Julia and Shastri, V Prasad
Journal: Journal of the American Chemical Society (2014): 10527-33
Rapid labeling of metabolically engineered cell-surface glycoconjugates with a carbamate-linked cyclopropene reporter.
Authors: Späte, Anne-Katrin and Bußkamp, Holger and Niederwieser, Andrea and Schart, Verena F and Marx, Andreas and Wittmann, Valentin
Journal: Bioconjugate chemistry (2014): 147-54
Authors: Späte, Anne-Katrin and Bußkamp, Holger and Niederwieser, Andrea and Schart, Verena F and Marx, Andreas and Wittmann, Valentin
Journal: Bioconjugate chemistry (2014): 147-54
Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation.
Authors: Burnham-Marusich, Amanda R and Plechaty, Anna M and Berninsone, Patricia M
Journal: Electrophoresis (2014): 2621-5
Authors: Burnham-Marusich, Amanda R and Plechaty, Anna M and Berninsone, Patricia M
Journal: Electrophoresis (2014): 2621-5
Stepwise orthogonal click chemistry toward fabrication of paclitaxel/galactose functionalized fluorescent nanoparticles for HepG2 cell targeting and delivery.
Authors: Lai, Chian-Hui and Chang, Tsung-Che and Chuang, Yung-Jen and Tzou, Der-Lii and Lin, Chun-Cheng
Journal: Bioconjugate chemistry (2013): 1698-709
Authors: Lai, Chian-Hui and Chang, Tsung-Che and Chuang, Yung-Jen and Tzou, Der-Lii and Lin, Chun-Cheng
Journal: Bioconjugate chemistry (2013): 1698-709