Cy3B alkyne

Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. However, Cy3 suffers low fluorescence intensity. It is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3B alkyne can be used for conjugations with azide-containing biological molecules. Cy3® is the trademark of GE Healthcare.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
Cy3B DBCO	560	571	120000¹	0.069
Cy3B TCO	560	571	120000¹	0.069
Cy3B Tetrazine	560	571	120000¹	0.069
AMCA Alkyne	346	434	19000	0.153
ICG alkyne	789	813	230000	0.076
XFD488 alkyne Same Structure to Alexa Fluor™ 488 alkyne	499	520	71000	0.11
XFD647 Alkyne	650	671	270000	0.03

Citations

View all 1 citations: Citation Explorer

Nanoscale DNA tracing reveals the self-organization mechanism of mitotic chromosomes
Authors: Beckwith, Kai Sandvold and Brunner, Andreas and Morero, Natalia Rosalia and Jungmann, Ralf and Ellenberg, Jan
Journal: Cell (2025)

References

View all 7 references: Citation Explorer

Sequence-Specific Post-Synthetic Oligonucleotide Labeling for Single-Molecule Fluorescence Applications.
Authors: Egloff, David and Oleinich, Igor A and Zhao, Meng and König, Sebastian L B and Sigel, Roland K O and Freisinger, Eva
Journal: ACS chemical biology (2016): 2558-67

Synthesis and labeling of α-(2,9)-trisialic acid with cyanine dyes for imaging of glycan-binding receptors on living cells.
Authors: Zhang, Xiao-tai and Gu, Zhen-yuan and Liu, Libing and Wang, Shu and Xing, Guo-wen
Journal: Chemical communications (Cambridge, England) (2015): 8606-9

Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation.
Authors: Burnham-Marusich, Amanda R and Plechaty, Anna M and Berninsone, Patricia M
Journal: Electrophoresis (2014): 2621-5

Rapid labeling of metabolically engineered cell-surface glycoconjugates with a carbamate-linked cyclopropene reporter.
Authors: Späte, Anne-Katrin and Bußkamp, Holger and Niederwieser, Andrea and Schart, Verena F and Marx, Andreas and Wittmann, Valentin
Journal: Bioconjugate chemistry (2014): 147-54

Clickable degradable aliphatic polyesters via copolymerization with alkyne epoxy esters: synthesis and postfunctionalization with organic dyes.
Authors: Teske, Nele S and Voigt, Julia and Shastri, V Prasad
Journal: Journal of the American Chemical Society (2014): 10527-33

Page updated on April 25, 2025

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Catalog Number	944
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Physical properties

Molecular weight	950.15
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.048
Correction Factor (280 nm)	0.069
Extinction coefficient (cm ^-1 M ^-1)	120000¹
Excitation (nm)	560
Emission (nm)	571
Quantum yield	0.58¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501