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Cy3 aldehyde

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Physical properties
Molecular weight919.00
Spectral properties
Correction Factor (260 nm)0.07
Correction Factor (280 nm)0.073
Extinction coefficient (cm -1 M -1)1500001
Excitation (nm)555
Emission (nm)569
Quantum yield0.151
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Cyanines
Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
Cy3 aldehyde is a reactive fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated Cy3 dye can be easily detected by a common fluorescence instrument under TRITC or Cy3 channel.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3 aldehyde to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM108.814 µL544.07 µL1.088 mL5.441 mL10.881 mL
5 mM21.763 µL108.814 µL217.628 µL1.088 mL2.176 mL
10 mM10.881 µL54.407 µL108.814 µL544.07 µL1.088 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectral properties

Correction Factor (260 nm)0.07
Correction Factor (280 nm)0.073
Extinction coefficient (cm -1 M -1)1500001
Excitation (nm)555
Emission (nm)569
Quantum yield0.151

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Cy3 tetrazine55556915000010.1510.070.073
Cy3 phosphoramidite55556915000010.1510.070.073
Cy3 tyramide55556915000010.1510.070.073
Fluorescein aldehyde [5-FAM aldehyde]49351783000-0.320.178
Rhodamine aldehyde [5-TAMRA aldehyde]55257890000-0.320.178
Cy5 aldehyde65167025000010.271, 0.420.020.03
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*499520710000.9210.300.11



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Authors: Chouchani, Edward T and Hurd, Thomas R and Nadtochiy, Sergiy M and Brookes, Paul S and Fearnley, Ian M and Lilley, Kathryn S and Smith, Robin A J and Murphy, Michael P
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