Cy3 aldehyde

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Physical properties

Molecular weight	919.00
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.07
Correction Factor (280 nm)	0.073
Extinction coefficient (cm ^-1 M ^-1)	150000¹
Excitation (nm)	555
Emission (nm)	569
Quantum yield	0.15¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

919.00

Correction Factor (260 nm)

0.07

Correction Factor (280 nm)

0.073

Extinction coefficient (cm ^-1 M ^-1)

150000¹

Excitation (nm)

555

Emission (nm)

569

Quantum yield

0.15¹

Cy3 aldehyde is a reactive fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated Cy3 dye can be easily detected by a common fluorescence instrument under TRITC or Cy3 channel.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3 aldehyde to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	108.814 µL	544.07 µL	1.088 mL	5.441 mL	10.881 mL
5 mM	21.763 µL	108.814 µL	217.628 µL	1.088 mL	2.176 mL
10 mM	10.881 µL	54.407 µL	108.814 µL	544.07 µL	1.088 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.07
Correction Factor (280 nm)	0.073
Extinction coefficient (cm ^-1 M ^-1)	150000¹
Excitation (nm)	555
Emission (nm)	569
Quantum yield	0.15¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Cy3 tetrazine	555	569	150000¹	0.15¹	0.07	0.073
Cy3 phosphoramidite	555	569	150000¹	0.15¹	0.07	0.073
Cy3 tyramide	555	569	150000¹	0.15¹	0.07	0.073
DBCO-Cy3	555	569	150000¹	0.15¹	0.07	0.073
Fluorescein aldehyde [5-FAM aldehyde]	493	517	83000	-	0.32	0.178
Rhodamine aldehyde [5-TAMRA aldehyde]	552	578	90000	-	0.32	0.178
Cy5 aldehyde	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
XFD488 aldehyde Same Structure to Alexa Fluor™ 488 aldehyde	490	520	71000	0.92¹	0.30	0.11

Images

Figure 1. Chemical structure for Cy3 aldehyde.

References

View all 15 references: Citation Explorer

Resonance Energy Transfer-Based Nucleic Acid Hybridization Assays on Paper-Based Platforms Using Emissive Nanoparticles as Donors.
Authors: Doughan, Samer and Noor, M Omair and Han, Yi and Krull, Ulrich J
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 301-326

Development of a DNA microarray-based multiplex assay of avian influenza virus subtypes H5, H7, H9, N1, and N2.
Authors: Shi, L and Sun, J-S and Yang, Z-P and Bao, H-M and Jiang, Y-P and Xiong, Y-Z and Cao, D and Yu, X-W and Chen, H-L and Zheng, S-M and Wang, X-R
Journal: Acta virologica (2014): 14-9

Development and application of antibody microarray for lymphocystis disease virus detection in fish.
Authors: Sheng, Xiuzhen and Xu, Xiaoli and Zhan, Wenbin
Journal: Journal of virological methods (2013): 243-9

The role of Src protein in the process formation of PC12 cells induced by the proteasome inhibitor MG-132.
Authors: Tarjányi, Oktávia and Berta, Gergely and Harci, Alexandra and Bacsa, Eszter B and Stark, Borbála and Pap, Marianna and Szeberényi, József and Sétáló, György
Journal: Neurochemistry international (2013): 413-22

A multiplexed screening method for agonists and antagonists of the estrogen receptor protein.
Authors: Li, Zhonghui and Yan, Ming and Li, Zhoumin and Vuki, Maika and Wu, Dan and Liu, Fei and Zhong, Wenying and Zhang, Luyong and Xu, Danke
Journal: Analytical and bioanalytical chemistry (2012): 1373-84

Fluorescence enhancement of silver nanoparticle hybrid probes and ultrasensitive detection of IgE.
Authors: Li, Hui and Qiang, Weibing and Vuki, Maika and Xu, Danke and Chen, Hong-Yuan
Journal: Analytical chemistry (2011): 8945-52

Identification of S-nitrosated mitochondrial proteins by S-nitrosothiol difference in gel electrophoresis (SNO-DIGE): implications for the regulation of mitochondrial function by reversible S-nitrosation.
Authors: Chouchani, Edward T and Hurd, Thomas R and Nadtochiy, Sergiy M and Brookes, Paul S and Fearnley, Ian M and Lilley, Kathryn S and Smith, Robin A J and Murphy, Michael P
Journal: The Biochemical journal (2010): 49-59

Protein thiol oxidation in murine airway epithelial cells in response to naphthalene or diethyl maleate.
Authors: Spiess, Page C and Morin, Dexter and Williams, Chase R and Buckpitt, Alan R
Journal: American journal of respiratory cell and molecular biology (2010): 316-25

A novel in situ polymerase chain reaction hybridisation assay for the direct detection of bovine herpesvirus type 5 in formalin-fixed, paraffin-embedded tissues.
Authors: Cardoso, Tereza C and Gomes, Deriane E and Ferrari, Heitor F and Silva-Frade, Camila and Rosa, Ana C G and Andrade, Alexandre L and Luvizotto, Maria Cecília R
Journal: Journal of virological methods (2010): 509-12

Protein chip for detection of different HCV antibodies: preparation, quality control, and clinical evaluation.
Authors: Zhang, Wen and Huang, Jian and Zhou, Mei-Fen and Chen, Li-Yan and Ding, Ya-Ping and Cao, Heng-Jie and Geng, Yong-Yao and Wang, Sheng-Qi
Journal: Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biol (2005): 81-7