AAT Bioquest

Cy3 trans-cyclooctene [Cy3 TCO]

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Physical properties
Molecular weight940.26
Spectral properties
Correction Factor (260 nm)0.07
Correction Factor (280 nm)0.073
Extinction coefficient (cm -1 M -1)1500001
Excitation (nm)555
Emission (nm)569
Quantum yield0.151
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Cyanines
Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This inverse electron demand cycloaddition reaction has gained popularity due to the potential for extremely fast cycloaddition kinetics with TCO as the dienophile. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this poweful click reaction. Cy3-TCO has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. It is widely used for labeling peptides, proteins and oligos etc. Cy3 dyes have enhanced fluorescence upon binding to proteins.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3 trans-cyclooctene [Cy3 TCO] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM106.354 µL531.768 µL1.064 mL5.318 mL10.635 mL
5 mM21.271 µL106.354 µL212.707 µL1.064 mL2.127 mL
10 mM10.635 µL53.177 µL106.354 µL531.768 µL1.064 mL

Molarity calculator

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Spectral properties

Correction Factor (260 nm)0.07
Correction Factor (280 nm)0.073
Extinction coefficient (cm -1 M -1)1500001
Excitation (nm)555
Emission (nm)569
Quantum yield0.151

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)Correction Factor (482 nm)Correction Factor (565 nm)
Cy5 trans-cyclooctene [Cy5 TCO]65167025000010.271, 0.420.020.030.0090.09



View all 6 citations: Citation Explorer
AlbuCatcher for Long-Acting Therapeutics
Authors: Rho, Ji Hyun and Lee, Jae Hun and Kwon, Inchan
Journal: ACS Omega (2024)
Enhanced therapeutic potential of antibody fragment via IEDDA-mediated site-specific albumin conjugation
Authors: Go, Eun Byeol and Lee, Jae Hun and Cho, Jeong Haeng and Kwon, Na Hyun and Choi, Jong-il and Kwon, Inchan
Journal: Journal of Biological Engineering (2024): 1--12
Thermostable and Long-Circulating Albumin-Conjugated Arthrobacter globiformis Urate Oxidase
Authors: Yang, Byungseop and Kwon, Inchan
Journal: Pharmaceutics (2021): 1298
Therapeutic Pretargeting with Gold Nanoparticles as Drug Candidates for Boron Neutron Capture Therapy
Authors: Feiner, Irene VJ and Pulagam, Krishna R and G{\'o}mez-Vallejo, Vanessa and Zamacola, Kepa and Baz, Zuri{\~n}e and Caffarel, Mar{\'\i}a M and Lawrie, Charles H and Ruiz-de-Angulo, Ane and Carril, M{\'o}nica and Llop, Jordi
Journal: Particle \& Particle Systems Characterization (2020): 2000200
Core--shell patterning of synthetic hydrogels via interfacial bioorthogonal chemistry for spatial control of stem cell behavior
Authors: Dicker, KT and Song, J and Moore, AC and Zhang, H and Li, Y and Burris, DL and Jia, X and Fox, JM
Journal: Chemical science (2018): 5394--5404


View all 49 references: Citation Explorer
Use of click-chemistry in the development of peptidomimetic enzyme inhibitors
Authors: Fabbrizzi P, Menchi G, Guarna A, Trabocchi A.
Journal: Curr Med Chem (2014): 1467
A dynamic duo: pairing click chemistry and postpolymerization modification to design complex surfaces
Authors: Arnold RM, Patton DL, Popik VV, Locklin J.
Journal: Acc Chem Res (2014): 2999
Specific and quantitative labeling of biomolecules using click chemistry
Authors: Horisawa K., undefined
Journal: Front Physiol (2014): 457
'Click chemistry' for diagnosis: a patent review on exploitation of its emerging trends
Authors: M, undefined and hare A, Banerjee P, Bhutkar S, Hirwani R.
Journal: Expert Opin Ther Pat (2014): 1287
Applications of copper-catalyzed click chemistry in activity-based protein profiling
Authors: Martell J, Weerapana E.
Journal: Molecules (2014): 1378
Calixarene-based chemosensors by means of click chemistry
Authors: Song M, Sun Z, Han C, Tian D, Li H, Kim JS.
Journal: Chem Asian J (2014): 2344
Beyond click chemistry - supramolecular interactions of 1,2,3-triazoles
Authors: Schulze B, Schubert US.
Journal: Chem Soc Rev (2014): 2522
Applications of azide-based bioorthogonal click chemistry in glycobiology
Authors: Zhang X, Zhang Y.
Journal: Molecules (2013): 7145
Inverse electron demand Diels-Alder (iEDDA)-initiated conjugation: a (high) potential click chemistry scheme
Authors: Knall AC, Slugovc C.
Journal: Chem Soc Rev (2013): 5131
Click chemistry for drug development and diverse chemical-biology applications
Authors: Thirumurugan P, Matosiuk D, Jozwiak K.
Journal: Chem Rev (2013): 4905