Cy3 trans-cyclooctene [Cy3 TCO]

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	940.26
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.07
Correction Factor (280 nm)	0.073
Extinction coefficient (cm ^-1 M ^-1)	150000¹
Excitation (nm)	555
Emission (nm)	569
Quantum yield	0.15¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

940.26

Correction Factor (260 nm)

0.07

Correction Factor (280 nm)

0.073

Extinction coefficient (cm ^-1 M ^-1)

150000¹

Excitation (nm)

555

Emission (nm)

569

Quantum yield

0.15¹

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This inverse electron demand cycloaddition reaction has gained popularity due to the potential for extremely fast cycloaddition kinetics with TCO as the dienophile. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this poweful click reaction. Cy3-TCO has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. It is widely used for labeling peptides, proteins and oligos etc. Cy3 dyes have enhanced fluorescence upon binding to proteins.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3 trans-cyclooctene [Cy3 TCO] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	106.354 µL	531.768 µL	1.064 mL	5.318 mL	10.635 mL
5 mM	21.271 µL	106.354 µL	212.707 µL	1.064 mL	2.127 mL
10 mM	10.635 µL	53.177 µL	106.354 µL	531.768 µL	1.064 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.07
Correction Factor (280 nm)	0.073
Extinction coefficient (cm ^-1 M ^-1)	150000¹
Excitation (nm)	555
Emission (nm)	569
Quantum yield	0.15¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)	Correction Factor (482 nm)	Correction Factor (565 nm)
Cy5 trans-cyclooctene [Cy5 TCO]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09

Images

Figure 1. Chemical structure for Cy3 trans-cyclooctene [Cy3 TCO]

Citations

View all 4 citations: Citation Explorer

Thermostable and Long-Circulating Albumin-Conjugated Arthrobacter globiformis Urate Oxidase
Authors: Yang, Byungseop and Kwon, Inchan
Journal: Pharmaceutics (2021): 1298

Therapeutic Pretargeting with Gold Nanoparticles as Drug Candidates for Boron Neutron Capture Therapy
Authors: Feiner, Irene VJ and Pulagam, Krishna R and G{\'o}mez-Vallejo, Vanessa and Zamacola, Kepa and Baz, Zuri{\~n}e and Caffarel, Mar{\'\i}a M and Lawrie, Charles H and Ruiz-de-Angulo, Ane and Carril, M{\'o}nica and Llop, Jordi
Journal: Particle \& Particle Systems Characterization (2020): 2000200

Core--shell patterning of synthetic hydrogels via interfacial bioorthogonal chemistry for spatial control of stem cell behavior
Authors: Dicker, KT and Song, J and Moore, AC and Zhang, H and Li, Y and Burris, DL and Jia, X and Fox, JM
Journal: Chemical science (2018): 5394--5404

Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene--methyltetrazine reactions
Authors: Muraki, Michiro and Hirota, Kiyonori
Journal: BMC biotechnology (2017): 56

References

View all 49 references: Citation Explorer

Beyond click chemistry - supramolecular interactions of 1,2,3-triazoles
Authors: Schulze B, Schubert US.
Journal: Chem Soc Rev (2014): 2522

Calixarene-based chemosensors by means of click chemistry
Authors: Song M, Sun Z, Han C, Tian D, Li H, Kim JS.
Journal: Chem Asian J (2014): 2344

Use of click-chemistry in the development of peptidomimetic enzyme inhibitors
Authors: Fabbrizzi P, Menchi G, Guarna A, Trabocchi A.
Journal: Curr Med Chem (2014): 1467

Applications of copper-catalyzed click chemistry in activity-based protein profiling
Authors: Martell J, Weerapana E.
Journal: Molecules (2014): 1378

'Click chemistry' for diagnosis: a patent review on exploitation of its emerging trends
Authors: M, undefined and hare A, Banerjee P, Bhutkar S, Hirwani R.
Journal: Expert Opin Ther Pat (2014): 1287

Specific and quantitative labeling of biomolecules using click chemistry
Authors: Horisawa K., undefined
Journal: Front Physiol (2014): 457

A dynamic duo: pairing click chemistry and postpolymerization modification to design complex surfaces
Authors: Arnold RM, Patton DL, Popik VV, Locklin J.
Journal: Acc Chem Res (2014): 2999

Applications of azide-based bioorthogonal click chemistry in glycobiology
Authors: Zhang X, Zhang Y.
Journal: Molecules (2013): 7145

Peptide conjugation via CuAAC 'click' chemistry
Authors: Ahmad Fuaad AA, Azmi F, Skwarczynski M, Toth I.
Journal: Molecules (2013): 13148

In situ click chemistry: from small molecule discovery to synthetic antibodies
Authors: Millward SW, Agnew HD, Lai B, Lee SS, Lim J, Nag A, Pitram S, Rohde R, Heath JR.
Journal: Integr Biol (Camb) (2013): 87