Cy5 aldehyde

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Physical properties

Molecular weight	945.04
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.02
Correction Factor (280 nm)	0.03
Correction Factor (482 nm)	0.009
Correction Factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27¹, 0.4²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy5 aldehyde to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	105.816 µL	529.078 µL	1.058 mL	5.291 mL	10.582 mL
5 mM	21.163 µL	105.816 µL	211.631 µL	1.058 mL	2.116 mL
10 mM	10.582 µL	52.908 µL	105.816 µL	529.078 µL	1.058 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Spectral properties

Correction Factor (260 nm)	0.02
Correction Factor (280 nm)	0.03
Correction Factor (482 nm)	0.009
Correction Factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27¹, 0.4²

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)	Correction Factor (482 nm)	Correction Factor (565 nm)
Cy5 tertrazine [Cy5 tertrazine]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cy5 phosphoramidite	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cy5 tyramide	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cy5 tetrazine	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
DBCO-Cy5	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Fluorescein aldehyde [5-FAM aldehyde]	493	517	83000	-	0.32	0.178	-	-
Rhodamine aldehyde [5-TAMRA aldehyde]	552	578	90000	-	0.32	0.178	-	-
Cy3 aldehyde	555	569	150000¹	0.15¹	0.07	0.073	-	-
XFD488 aldehyde Same Structure to Alexa Fluor™ 488 aldehyde	499	520	71000	0.92¹	0.30	0.11	-	-

Images

Figure 1. Chemical structure for Cy5 aldehyde.

References

View all 13 references: Citation Explorer

[Microanalysis of Acetaldehyde Dehydrogenase 2 Genotype Based on SNaPShot-FCA].
Authors: Zeng, Shu-Shu and Gao, Xiu-Feng and Zhang, You and Feng, Chuan-Rui and Luo, Ya-Xiong and Wu, Xiao-Ting and Li, Yong-Sheng
Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (2019): 737-742

pH responsible and fluorescent Cy5.5-PEG-g-A-HA/CDDP complex nanoparticles: synthesis, characterization, and application for targeted drug delivery.
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Liu, Chun and Zhang, Qiqing
Journal: Journal of materials science. Materials in medicine (2019): 58

Tumoral Acidic pH-Responsive cis-Diaminodichloroplatinum-Incorporated Cy5.5-PEG- g-A-HA Nanoparticles for Targeting Delivery of CDDP against Cervical Cancer.
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Zhang, Qiqing
Journal: ACS applied materials & interfaces (2018): 26882-26892

Sensitive Detection of Polynucleotide Kinase Activity by Paper-Based Fluorescence Assay with λ Exonuclease Assistance.
Authors: Zhang, Hua and Zhao, Zhen and Lei, Zhen and Wang, Zhenxin
Journal: Analytical chemistry (2016): 11358-11363

Sensitive detection of histamine using fluorescently labeled oxido-reductases.
Authors: Gustiananda, Marsia and Andreoni, Alessio and Tabares, Leandro C and Tepper, Armand W J W and Fortunato, Luca and Aartsma, Thijs J and Canters, Gerard W
Journal: Biosensors & bioelectronics (2012): 419-25

An efficient strategy for sequencing-by-synthesis.
Authors: Gao, Li and Lu, Hua and Xu, Lijian and Lu, Zuhong
Journal: Journal of nanoscience and nanotechnology (2010): 2988-93

Identification of S-nitrosated mitochondrial proteins by S-nitrosothiol difference in gel electrophoresis (SNO-DIGE): implications for the regulation of mitochondrial function by reversible S-nitrosation.
Authors: Chouchani, Edward T and Hurd, Thomas R and Nadtochiy, Sergiy M and Brookes, Paul S and Fearnley, Ian M and Lilley, Kathryn S and Smith, Robin A J and Murphy, Michael P
Journal: The Biochemical journal (2010): 49-59

Protein thiol oxidation in murine airway epithelial cells in response to naphthalene or diethyl maleate.
Authors: Spiess, Page C and Morin, Dexter and Williams, Chase R and Buckpitt, Alan R
Journal: American journal of respiratory cell and molecular biology (2010): 316-25

Chemical derivatization of compact disc polycarbonate surfaces for SNPs detection.
Authors: Bañuls, María-José and García-Piñón, Francisco and Puchades, Rosa and Maquieira, Angel
Journal: Bioconjugate chemistry (2008): 665-72

Identification and characterization of Clostridium perfringens using single target DNA microarray chip.
Authors: Al-Khaldi, Sufian F and Villanueva, Doralis and Chizhikov, Vladimir
Journal: International journal of food microbiology (2004): 289-96