Cy5 aldehyde
Cy5 aldehyde is a reactive red fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated Cy5 dye can be easily detected by a common fluorescence instrument under Cy5 channel.
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (482 nm) | Correction Factor (565 nm) |
Cy5 tertrazine [Cy5 tertrazine] | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
Cy5 phosphoramidite | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
Cy5 tyramide | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
Cy5 tetrazine | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
DBCO-Cy5 | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
Fluorescein aldehyde [5-FAM aldehyde] | 493 | 517 | 83000 | - | 0.32 | 0.178 | - | - |
Rhodamine aldehyde [5-TAMRA aldehyde] | 552 | 578 | 90000 | - | 0.32 | 0.178 | - | - |
Cy3 aldehyde | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 | - | - |
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 | - | - |
References
View all 13 references: Citation Explorer
pH responsible and fluorescent Cy5.5-PEG-g-A-HA/CDDP complex nanoparticles: synthesis, characterization, and application for targeted drug delivery.
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Liu, Chun and Zhang, Qiqing
Journal: Journal of materials science. Materials in medicine (2019): 58
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Liu, Chun and Zhang, Qiqing
Journal: Journal of materials science. Materials in medicine (2019): 58
[Microanalysis of Acetaldehyde Dehydrogenase 2 Genotype Based on SNaPShot-FCA].
Authors: Zeng, Shu-Shu and Gao, Xiu-Feng and Zhang, You and Feng, Chuan-Rui and Luo, Ya-Xiong and Wu, Xiao-Ting and Li, Yong-Sheng
Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (2019): 737-742
Authors: Zeng, Shu-Shu and Gao, Xiu-Feng and Zhang, You and Feng, Chuan-Rui and Luo, Ya-Xiong and Wu, Xiao-Ting and Li, Yong-Sheng
Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (2019): 737-742
Tumoral Acidic pH-Responsive cis-Diaminodichloroplatinum-Incorporated Cy5.5-PEG- g-A-HA Nanoparticles for Targeting Delivery of CDDP against Cervical Cancer.
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Zhang, Qiqing
Journal: ACS applied materials & interfaces (2018): 26882-26892
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Zhang, Qiqing
Journal: ACS applied materials & interfaces (2018): 26882-26892
Sensitive Detection of Polynucleotide Kinase Activity by Paper-Based Fluorescence Assay with λ Exonuclease Assistance.
Authors: Zhang, Hua and Zhao, Zhen and Lei, Zhen and Wang, Zhenxin
Journal: Analytical chemistry (2016): 11358-11363
Authors: Zhang, Hua and Zhao, Zhen and Lei, Zhen and Wang, Zhenxin
Journal: Analytical chemistry (2016): 11358-11363
Sensitive detection of histamine using fluorescently labeled oxido-reductases.
Authors: Gustiananda, Marsia and Andreoni, Alessio and Tabares, Leandro C and Tepper, Armand W J W and Fortunato, Luca and Aartsma, Thijs J and Canters, Gerard W
Journal: Biosensors & bioelectronics (2012): 419-25
Authors: Gustiananda, Marsia and Andreoni, Alessio and Tabares, Leandro C and Tepper, Armand W J W and Fortunato, Luca and Aartsma, Thijs J and Canters, Gerard W
Journal: Biosensors & bioelectronics (2012): 419-25
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