Cy5 aldehyde
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 945.04 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.02 |
Correction Factor (280 nm) | 0.03 |
Correction Factor (482 nm) | 0.009 |
Correction Factor (565 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 651 |
Emission (nm) | 670 |
Quantum yield | 0.271, 0.42 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
See also: Cyanines
Molecular weight 945.04 | Correction Factor (260 nm) 0.02 | Correction Factor (280 nm) 0.03 | Correction Factor (482 nm) 0.009 | Correction Factor (565 nm) 0.09 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 651 | Emission (nm) 670 | Quantum yield 0.271, 0.42 |
Cy5 aldehyde is a reactive red fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated Cy5 dye can be easily detected by a common fluorescence instrument under Cy5 channel.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cy5 aldehyde to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 105.816 µL | 529.078 µL | 1.058 mL | 5.291 mL | 10.582 mL |
5 mM | 21.163 µL | 105.816 µL | 211.631 µL | 1.058 mL | 2.116 mL |
10 mM | 10.582 µL | 52.908 µL | 105.816 µL | 529.078 µL | 1.058 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.02 |
Correction Factor (280 nm) | 0.03 |
Correction Factor (482 nm) | 0.009 |
Correction Factor (565 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 651 |
Emission (nm) | 670 |
Quantum yield | 0.271, 0.42 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (482 nm) | Correction Factor (565 nm) |
Cy5 tertrazine [Cy5 tertrazine] | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
Cy5 phosphoramidite | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
Cy5 tyramide | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
Cy5 tetrazine | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
DBCO-Cy5 | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
Fluorescein aldehyde [5-FAM aldehyde] | 493 | 517 | 83000 | - | 0.32 | 0.178 | - | - |
Rhodamine aldehyde [5-TAMRA aldehyde] | 552 | 578 | 90000 | - | 0.32 | 0.178 | - | - |
Cy3 aldehyde | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 | - | - |
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 | - | - |
References
View all 13 references: Citation Explorer
[Microanalysis of Acetaldehyde Dehydrogenase 2 Genotype Based on SNaPShot-FCA].
Authors: Zeng, Shu-Shu and Gao, Xiu-Feng and Zhang, You and Feng, Chuan-Rui and Luo, Ya-Xiong and Wu, Xiao-Ting and Li, Yong-Sheng
Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (2019): 737-742
Authors: Zeng, Shu-Shu and Gao, Xiu-Feng and Zhang, You and Feng, Chuan-Rui and Luo, Ya-Xiong and Wu, Xiao-Ting and Li, Yong-Sheng
Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (2019): 737-742
pH responsible and fluorescent Cy5.5-PEG-g-A-HA/CDDP complex nanoparticles: synthesis, characterization, and application for targeted drug delivery.
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Liu, Chun and Zhang, Qiqing
Journal: Journal of materials science. Materials in medicine (2019): 58
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Liu, Chun and Zhang, Qiqing
Journal: Journal of materials science. Materials in medicine (2019): 58
Tumoral Acidic pH-Responsive cis-Diaminodichloroplatinum-Incorporated Cy5.5-PEG- g-A-HA Nanoparticles for Targeting Delivery of CDDP against Cervical Cancer.
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Zhang, Qiqing
Journal: ACS applied materials & interfaces (2018): 26882-26892
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Zhang, Qiqing
Journal: ACS applied materials & interfaces (2018): 26882-26892
Sensitive Detection of Polynucleotide Kinase Activity by Paper-Based Fluorescence Assay with λ Exonuclease Assistance.
Authors: Zhang, Hua and Zhao, Zhen and Lei, Zhen and Wang, Zhenxin
Journal: Analytical chemistry (2016): 11358-11363
Authors: Zhang, Hua and Zhao, Zhen and Lei, Zhen and Wang, Zhenxin
Journal: Analytical chemistry (2016): 11358-11363
Sensitive detection of histamine using fluorescently labeled oxido-reductases.
Authors: Gustiananda, Marsia and Andreoni, Alessio and Tabares, Leandro C and Tepper, Armand W J W and Fortunato, Luca and Aartsma, Thijs J and Canters, Gerard W
Journal: Biosensors & bioelectronics (2012): 419-25
Authors: Gustiananda, Marsia and Andreoni, Alessio and Tabares, Leandro C and Tepper, Armand W J W and Fortunato, Luca and Aartsma, Thijs J and Canters, Gerard W
Journal: Biosensors & bioelectronics (2012): 419-25
An efficient strategy for sequencing-by-synthesis.
Authors: Gao, Li and Lu, Hua and Xu, Lijian and Lu, Zuhong
Journal: Journal of nanoscience and nanotechnology (2010): 2988-93
Authors: Gao, Li and Lu, Hua and Xu, Lijian and Lu, Zuhong
Journal: Journal of nanoscience and nanotechnology (2010): 2988-93
Identification of S-nitrosated mitochondrial proteins by S-nitrosothiol difference in gel electrophoresis (SNO-DIGE): implications for the regulation of mitochondrial function by reversible S-nitrosation.
Authors: Chouchani, Edward T and Hurd, Thomas R and Nadtochiy, Sergiy M and Brookes, Paul S and Fearnley, Ian M and Lilley, Kathryn S and Smith, Robin A J and Murphy, Michael P
Journal: The Biochemical journal (2010): 49-59
Authors: Chouchani, Edward T and Hurd, Thomas R and Nadtochiy, Sergiy M and Brookes, Paul S and Fearnley, Ian M and Lilley, Kathryn S and Smith, Robin A J and Murphy, Michael P
Journal: The Biochemical journal (2010): 49-59
Protein thiol oxidation in murine airway epithelial cells in response to naphthalene or diethyl maleate.
Authors: Spiess, Page C and Morin, Dexter and Williams, Chase R and Buckpitt, Alan R
Journal: American journal of respiratory cell and molecular biology (2010): 316-25
Authors: Spiess, Page C and Morin, Dexter and Williams, Chase R and Buckpitt, Alan R
Journal: American journal of respiratory cell and molecular biology (2010): 316-25
Chemical derivatization of compact disc polycarbonate surfaces for SNPs detection.
Authors: Bañuls, María-José and García-Piñón, Francisco and Puchades, Rosa and Maquieira, Angel
Journal: Bioconjugate chemistry (2008): 665-72
Authors: Bañuls, María-José and García-Piñón, Francisco and Puchades, Rosa and Maquieira, Angel
Journal: Bioconjugate chemistry (2008): 665-72
Identification and characterization of Clostridium perfringens using single target DNA microarray chip.
Authors: Al-Khaldi, Sufian F and Villanueva, Doralis and Chizhikov, Vladimir
Journal: International journal of food microbiology (2004): 289-96
Authors: Al-Khaldi, Sufian F and Villanueva, Doralis and Chizhikov, Vladimir
Journal: International journal of food microbiology (2004): 289-96