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Cy5 aldehyde

Cy5 aldehyde is a reactive red fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated Cy5 dye can be easily detected by a common fluorescence instrument under Cy5 channel.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)Correction Factor (482 nm)Correction Factor (565 nm)
Cy5 tertrazine [Cy5 tertrazine]65167025000010.271, 0.420.020.030.0090.09
Cy5 phosphoramidite65167025000010.271, 0.420.020.030.0090.09
Cy5 tyramide65167025000010.271, 0.420.020.030.0090.09
Cy5 tetrazine65167025000010.271, 0.420.020.030.0090.09
DBCO-Cy565167025000010.271, 0.420.020.030.0090.09
Fluorescein aldehyde [5-FAM aldehyde]49351783000-0.320.178--
Rhodamine aldehyde [5-TAMRA aldehyde]55257890000-0.320.178--
Cy3 aldehyde55556915000010.1510.070.073--
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*499520710000.9210.300.11--

References

View all 13 references: Citation Explorer
pH responsible and fluorescent Cy5.5-PEG-g-A-HA/CDDP complex nanoparticles: synthesis, characterization, and application for targeted drug delivery.
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Liu, Chun and Zhang, Qiqing
Journal: Journal of materials science. Materials in medicine (2019): 58
[Microanalysis of Acetaldehyde Dehydrogenase 2 Genotype Based on SNaPShot-FCA].
Authors: Zeng, Shu-Shu and Gao, Xiu-Feng and Zhang, You and Feng, Chuan-Rui and Luo, Ya-Xiong and Wu, Xiao-Ting and Li, Yong-Sheng
Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (2019): 737-742
Tumoral Acidic pH-Responsive cis-Diaminodichloroplatinum-Incorporated Cy5.5-PEG- g-A-HA Nanoparticles for Targeting Delivery of CDDP against Cervical Cancer.
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Zhang, Qiqing
Journal: ACS applied materials & interfaces (2018): 26882-26892
Sensitive Detection of Polynucleotide Kinase Activity by Paper-Based Fluorescence Assay with λ Exonuclease Assistance.
Authors: Zhang, Hua and Zhao, Zhen and Lei, Zhen and Wang, Zhenxin
Journal: Analytical chemistry (2016): 11358-11363
Sensitive detection of histamine using fluorescently labeled oxido-reductases.
Authors: Gustiananda, Marsia and Andreoni, Alessio and Tabares, Leandro C and Tepper, Armand W J W and Fortunato, Luca and Aartsma, Thijs J and Canters, Gerard W
Journal: Biosensors & bioelectronics (2012): 419-25
Page updated on December 11, 2024

Ordering information

Price
Unit size
5 mg
1 mg
Catalog Number
90119012
Quantity
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Physical properties

Molecular weight

945.04

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.02

Correction Factor (280 nm)

0.03

Correction Factor (482 nm)

0.009

Correction Factor (565 nm)

0.09

Extinction coefficient (cm -1 M -1)

2500001

Excitation (nm)

651

Emission (nm)

670

Quantum yield

0.271, 0.42

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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