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Cy5.5 biotin conjugate
This bifunctional Cy5.5 biotin conjugate demonstrates excellent avidin binding and strong fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. Cy5.5 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and emission. Cy5.5 Biotin is a water-soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10.
Chemical structure for Cy5.5 biotin conjugate.
Chemical structure for Cy5.5 biotin conjugate.
CatalogSize
Price
Quantity
31055 mg
Price
 
Physical properties

Molecular weight1299.45
SolventWater
Spectral properties

Correction factor (260 nm)0.05
Correction factor (280 nm)0.101
Correction factor (482 nm)0.0017
Correction factor (565 nm)0.047
Correction factor (650 nm)0.454
Extinction coefficient (cm -1 M -1)
250000
Excitation (nm)683
Emission (nm)703
Quantum yield
0.27
Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 13, 2025