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Cy5.5 biotin conjugate
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1299.45 |
| Solvent | Water |
Spectral properties
| Correction Factor (260 nm) | 0.05 |
| Correction Factor (280 nm) | 0.101 |
| Correction Factor (482 nm) | 0.0017 |
| Correction Factor (565 nm) | 0.047 |
| Correction Factor (650 nm) | 0.454 |
| Extinction coefficient (cm -1 M -1) | 250000 |
| Excitation (nm) | 683 |
| Emission (nm) | 703 |
| Quantum yield | 0.27 |
Storage, safety and handling
| Certificate of Origin | Download PDF |
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
See also: Cyanines
Molecular weight 1299.45 | Correction Factor (260 nm) 0.05 | Correction Factor (280 nm) 0.101 | Correction Factor (482 nm) 0.0017 | Correction Factor (565 nm) 0.047 | Correction Factor (650 nm) 0.454 | Extinction coefficient (cm -1 M -1) 250000 | Excitation (nm) 683 | Emission (nm) 703 | Quantum yield 0.27 |
This bifunctional Cy5.5 biotin conjugate demonstrates excellent avidin binding and strong fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. Cy5.5 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and emission. Cy5.5 Biotin is a water-soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Cy5.5 biotin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 76.956 µL | 384.778 µL | 769.556 µL | 3.848 mL | 7.696 mL |
| 5 mM | 15.391 µL | 76.956 µL | 153.911 µL | 769.556 µL | 1.539 mL |
| 10 mM | 7.696 µL | 38.478 µL | 76.956 µL | 384.778 µL | 769.556 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.05 |
| Correction Factor (280 nm) | 0.101 |
| Correction Factor (482 nm) | 0.0017 |
| Correction Factor (565 nm) | 0.047 |
| Correction Factor (650 nm) | 0.454 |
| Extinction coefficient (cm -1 M -1) | 250000 |
| Excitation (nm) | 683 |
| Emission (nm) | 703 |
| Quantum yield | 0.27 |
Images
Figure 1. Chemical structure for Cy5.5 biotin conjugate.
References
View all 16 references: Citation Explorer
Carboxylesterase-Cleavable Biotinylated Nanoparticle for Tumor-Dual Targeted Imaging.
Authors: Chen, Peiyao and Kuang, Wen and Zheng, Zhen and Yang, Shuye and Liu, Yaling and Su, Lanhong and Zhao, Kui and Liang, Gaolin
Journal: Theranostics (2019): 7359-7369
Authors: Chen, Peiyao and Kuang, Wen and Zheng, Zhen and Yang, Shuye and Liu, Yaling and Su, Lanhong and Zhao, Kui and Liang, Gaolin
Journal: Theranostics (2019): 7359-7369
High-affinity peptide ligand LXY30 for targeting α3β1 integrin in non-small cell lung cancer.
Authors: Xiao, Wenwu and Ma, Weijie and Wei, Sixi and Li, Qianping and Liu, Ruiwu and Carney, Randy P and Yang, Kevin and Lee, Joyce and Nyugen, Alan and Yoneda, Ken Y and Lam, Kit S and Li, Tianhong
Journal: Journal of hematology & oncology (2019): 56
Authors: Xiao, Wenwu and Ma, Weijie and Wei, Sixi and Li, Qianping and Liu, Ruiwu and Carney, Randy P and Yang, Kevin and Lee, Joyce and Nyugen, Alan and Yoneda, Ken Y and Lam, Kit S and Li, Tianhong
Journal: Journal of hematology & oncology (2019): 56
Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues.
Authors: Tulloch, Lindsay B and Menzies, Stefanie K and Fraser, Andrew L and Gould, Eoin R and King, Elizabeth F and Zacharova, Marija K and Florence, Gordon J and Smith, Terry K
Journal: PLoS neglected tropical diseases (2017): e0005886
Authors: Tulloch, Lindsay B and Menzies, Stefanie K and Fraser, Andrew L and Gould, Eoin R and King, Elizabeth F and Zacharova, Marija K and Florence, Gordon J and Smith, Terry K
Journal: PLoS neglected tropical diseases (2017): e0005886
Intracellular Proteolytic Disassembly of Self-Quenched Near-Infrared Nanoparticles Turning Fluorescence on for Tumor-Targeted Imaging.
Authors: Jiang, Jinhui and Zhao, Zhibin and Hai, Zijuan and Wang, Hongyong and Liang, Gaolin
Journal: Analytical chemistry (2017): 9625-9628
Authors: Jiang, Jinhui and Zhao, Zhibin and Hai, Zijuan and Wang, Hongyong and Liang, Gaolin
Journal: Analytical chemistry (2017): 9625-9628
Surface modification of PLGA nanoparticles with biotinylated chitosan for the sustained in vitro release and the enhanced cytotoxicity of epirubicin.
Authors: Chen, Hongli and Xie, Li Qin and Qin, Jingwen and Jia, Yajing and Cai, Xinhua and Nan, WenBin and Yang, Wancai and Lv, Feng and Zhang, Qi Qing
Journal: Colloids and surfaces. B, Biointerfaces (2016): 1-9
Authors: Chen, Hongli and Xie, Li Qin and Qin, Jingwen and Jia, Yajing and Cai, Xinhua and Nan, WenBin and Yang, Wancai and Lv, Feng and Zhang, Qi Qing
Journal: Colloids and surfaces. B, Biointerfaces (2016): 1-9
SPECT/NIRF Dual Modality Imaging for Detection of Intraperitoneal Colon Tumor with an Avidin/Biotin Pretargeting System.
Authors: Dong, Chengyan and Yang, Sujuan and Shi, Jiyun and Zhao, Huiyun and Zhong, Lijun and Liu, Zhaofei and Jia, Bing and Wang, Fan
Journal: Scientific reports (2016): 18905
Authors: Dong, Chengyan and Yang, Sujuan and Shi, Jiyun and Zhao, Huiyun and Zhong, Lijun and Liu, Zhaofei and Jia, Bing and Wang, Fan
Journal: Scientific reports (2016): 18905
New paradigm for tumor theranostic methodology using bacteria-based microrobot.
Authors: Park, Sung Jun and Park, Seung-Hwan and Cho, Sunghoon and Kim, Deok-Mi and Lee, Yeonkyung and Ko, Seong Young and Hong, Yeongjin and Choy, Hyon E and Min, Jung-Joon and Park, Jong-Oh and Park, Sukho
Journal: Scientific reports (2013): 3394
Authors: Park, Sung Jun and Park, Seung-Hwan and Cho, Sunghoon and Kim, Deok-Mi and Lee, Yeonkyung and Ko, Seong Young and Hong, Yeongjin and Choy, Hyon E and Min, Jung-Joon and Park, Jong-Oh and Park, Sukho
Journal: Scientific reports (2013): 3394
Comparing the intracellular fate of components within a noncovalent streptavidin nanoparticle with covalent conjugation.
Authors: Liu, Yuxia and Cheng, Dengfeng and Liu, Xinrong and Liu, Guozheng and Dou, Shuping and Xiao, Nan and Chen, Ling and Rusckowski, Mary and Hnatowich, Donald J
Journal: Nuclear medicine and biology (2012): 101-7
Authors: Liu, Yuxia and Cheng, Dengfeng and Liu, Xinrong and Liu, Guozheng and Dou, Shuping and Xiao, Nan and Chen, Ling and Rusckowski, Mary and Hnatowich, Donald J
Journal: Nuclear medicine and biology (2012): 101-7
Uniform aligned bioconjugation of biomolecule motifs for integration within microfabricated microfluidic devices.
Authors: Braddick, Lucy M and Garland, Patrick J and Praeger, Matthew F and Butement, Jonathan and Friedrich, Daniel and Morgan, David J and Melvin, Tracy
Journal: Analytical biochemistry (2012): 195-205
Authors: Braddick, Lucy M and Garland, Patrick J and Praeger, Matthew F and Butement, Jonathan and Friedrich, Daniel and Morgan, David J and Melvin, Tracy
Journal: Analytical biochemistry (2012): 195-205
EGF receptor targeted tumor imaging with biotin-PEG-EGF linked to (99m)Tc-HYNIC labeled avidin and streptavidin.
Authors: Jung, Kyung-Ho and Park, Jin Won and Paik, Jin-Young and Quach, Cung Hoa Thien and Choe, Yearn Seong and Lee, Kyung-Han
Journal: Nuclear medicine and biology (2012): 1122-7
Authors: Jung, Kyung-Ho and Park, Jin Won and Paik, Jin-Young and Quach, Cung Hoa Thien and Choe, Yearn Seong and Lee, Kyung-Han
Journal: Nuclear medicine and biology (2012): 1122-7