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Cy5.5 biotin conjugate

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Physical properties
Molecular weight1299.45
Spectral properties
Correction Factor (260 nm)0.05
Correction Factor (280 nm)0.101
Correction Factor (482 nm)0.0017
Correction Factor (565 nm)0.047
Correction Factor (650 nm)0.454
Extinction coefficient (cm -1 M -1)250000
Excitation (nm)683
Emission (nm)703
Quantum yield0.27
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Show More (83)


See also: Cyanines
Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Correction Factor (482 nm)
Correction Factor (565 nm)
Correction Factor (650 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
This bifunctional Cy5.5 biotin conjugate demonstrates excellent avidin binding and strong fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. Cy5.5 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and emission. Cy5.5 Biotin is a water-soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cy5.5 biotin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM76.956 µL384.778 µL769.556 µL3.848 mL7.696 mL
5 mM15.391 µL76.956 µL153.911 µL769.556 µL1.539 mL
10 mM7.696 µL38.478 µL76.956 µL384.778 µL769.556 µL

Molarity calculator

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Spectral properties

Correction Factor (260 nm)0.05
Correction Factor (280 nm)0.101
Correction Factor (482 nm)0.0017
Correction Factor (565 nm)0.047
Correction Factor (650 nm)0.454
Extinction coefficient (cm -1 M -1)250000
Excitation (nm)683
Emission (nm)703
Quantum yield0.27



View all 16 references: Citation Explorer
High-affinity peptide ligand LXY30 for targeting α3β1 integrin in non-small cell lung cancer.
Authors: Xiao, Wenwu and Ma, Weijie and Wei, Sixi and Li, Qianping and Liu, Ruiwu and Carney, Randy P and Yang, Kevin and Lee, Joyce and Nyugen, Alan and Yoneda, Ken Y and Lam, Kit S and Li, Tianhong
Journal: Journal of hematology & oncology (2019): 56
Carboxylesterase-Cleavable Biotinylated Nanoparticle for Tumor-Dual Targeted Imaging.
Authors: Chen, Peiyao and Kuang, Wen and Zheng, Zhen and Yang, Shuye and Liu, Yaling and Su, Lanhong and Zhao, Kui and Liang, Gaolin
Journal: Theranostics (2019): 7359-7369
Intracellular Proteolytic Disassembly of Self-Quenched Near-Infrared Nanoparticles Turning Fluorescence on for Tumor-Targeted Imaging.
Authors: Jiang, Jinhui and Zhao, Zhibin and Hai, Zijuan and Wang, Hongyong and Liang, Gaolin
Journal: Analytical chemistry (2017): 9625-9628
Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues.
Authors: Tulloch, Lindsay B and Menzies, Stefanie K and Fraser, Andrew L and Gould, Eoin R and King, Elizabeth F and Zacharova, Marija K and Florence, Gordon J and Smith, Terry K
Journal: PLoS neglected tropical diseases (2017): e0005886
SPECT/NIRF Dual Modality Imaging for Detection of Intraperitoneal Colon Tumor with an Avidin/Biotin Pretargeting System.
Authors: Dong, Chengyan and Yang, Sujuan and Shi, Jiyun and Zhao, Huiyun and Zhong, Lijun and Liu, Zhaofei and Jia, Bing and Wang, Fan
Journal: Scientific reports (2016): 18905
Surface modification of PLGA nanoparticles with biotinylated chitosan for the sustained in vitro release and the enhanced cytotoxicity of epirubicin.
Authors: Chen, Hongli and Xie, Li Qin and Qin, Jingwen and Jia, Yajing and Cai, Xinhua and Nan, WenBin and Yang, Wancai and Lv, Feng and Zhang, Qi Qing
Journal: Colloids and surfaces. B, Biointerfaces (2016): 1-9
New paradigm for tumor theranostic methodology using bacteria-based microrobot.
Authors: Park, Sung Jun and Park, Seung-Hwan and Cho, Sunghoon and Kim, Deok-Mi and Lee, Yeonkyung and Ko, Seong Young and Hong, Yeongjin and Choy, Hyon E and Min, Jung-Joon and Park, Jong-Oh and Park, Sukho
Journal: Scientific reports (2013): 3394
EGF receptor targeted tumor imaging with biotin-PEG-EGF linked to (99m)Tc-HYNIC labeled avidin and streptavidin.
Authors: Jung, Kyung-Ho and Park, Jin Won and Paik, Jin-Young and Quach, Cung Hoa Thien and Choe, Yearn Seong and Lee, Kyung-Han
Journal: Nuclear medicine and biology (2012): 1122-7
Uniform aligned bioconjugation of biomolecule motifs for integration within microfabricated microfluidic devices.
Authors: Braddick, Lucy M and Garland, Patrick J and Praeger, Matthew F and Butement, Jonathan and Friedrich, Daniel and Morgan, David J and Melvin, Tracy
Journal: Analytical biochemistry (2012): 195-205
Comparing the intracellular fate of components within a noncovalent streptavidin nanoparticle with covalent conjugation.
Authors: Liu, Yuxia and Cheng, Dengfeng and Liu, Xinrong and Liu, Guozheng and Dou, Shuping and Xiao, Nan and Chen, Ling and Rusckowski, Mary and Hnatowich, Donald J
Journal: Nuclear medicine and biology (2012): 101-7