Name: Cy5.5NS succinimidyl ester
Brand: AAT Bioquest
SKU: 199
Price: 348 USD
Availability: InStock

Cy5.5NS succinimidyl ester

Cyanines are used in a wide variety of biological applications including comparative genomic hybridization and in gene chips. Depending on the structure, they cover the spectrum visible and IR wavelengths. Cy 3, Cy5, Cy5.5 and Cy7 are the most popular cyanine dyes. Cy5.5NS dyes have the spectral properties identical to Cy5.5, but do not have the four sulfonate groups of Cy5.5. Cy5.5NS dyes are not soluble in water while Cy5.5 dyes have high water solubility. AAT Bioquest offers the broadest selection of cyanine dyes. This non-sulfonated cyanine is not water soluble, and mainly used for labeling oligos, peptides and other small molecules. Cy5.5NS succinimidyl ester selectively reacts with aliphatic amines.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
199	10 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy5.5NS succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	136.924 µL	684.622 µL	1.369 mL	6.846 mL	13.692 mL
5 mM	27.385 µL	136.924 µL	273.849 µL	1.369 mL	2.738 mL
10 mM	13.692 µL	68.462 µL	136.924 µL	684.622 µL	1.369 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	730.33 g/mol	=	mL	x	mM	=	mmol

Citations

View all 12 citations: Citation Explorer

Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization

Authors:

Wang, Xiaoyan and Zhang, Yu and Xue, Wei and Wang, Hong and Qiu, Xiaozhong and Liu, Zonghua

Journal:

Journal of Biomaterials Applications (2017): 923--932

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy

Authors:

Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao

Journal:

Colloids and Surfaces B: Biointerfaces (2017)

Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics

Authors:

Guo, Yuxin and Zhang, Yang and Ma, Jinyuan and Li, Qi and Li, Yang and Zhou, Xinyi and Zhao, Dan and Song, Hua and Chen, Qing and Zhu, Xuan

Journal:

Journal of Controlled Release (2017)

Carboxymethyl Dextran-Stabilized Polyethylenimine-Poly (epsilon-caprolactone) Nanoparticles-Mediated Modulation of MicroRNA-34a Expression via Small-Molecule Modulator for Hepatocellular Carcinoma Therapy

Authors:

Deng, Xiongwei and Yin, Zhaoxia and Zhou, Zhixiang and Wang, Yihui and Zhang, Fang and Hu, Qin and Yang, Yishu and Lu, Jianqing and Wu, Yan and Sheng, Wang and others, undefined

Journal:

ACS applied materials & interfaces (2016): 17068--17079

Affinity-Controlled Protein Encapsulation into Sub-30 nm Telodendrimer Nanocarriers by Multivalent and Synergistic Interactions

Authors:

Wang, Xu and Shi, Changying and Zhang, Li and Bodman, Alexa and Guo, D and an , undefined and Wang, Lili and Hall, Walter A and Wilkens, Stephan and Luo, Juntao

Journal:

Biomaterials (2016)

References

View all 21 references: Citation Explorer

Excitation of Cy5 in self-assembled lipid bilayers using optical microresonators

Authors:

Freeman LM, Li S, Dayani Y, Choi HS, Malmstadt N, Armani AM.

Journal:

Appl Phys Lett (2011): 143703

Theranostic cRGD-BioShuttle Constructs Containing Temozolomide- and Cy7 For NIR-Imaging and Therapy

Authors:

Wiessler M, Hennrich U, Pipkorn R, Waldeck W, Cao L, Peter J, Ehemann V, Semmler W, Lammers T, Braun K.

Journal:

Theranostics (2011): 381

Rational approach to select small peptide molecular probes labeled with fluorescent cyanine dyes for in vivo optical imaging

Authors:

Berezin MY, Guo K, Akers W, Livingston J, Solomon M, Lee H, Liang K, Agee A, Achilefu S.

Journal:

Biochemistry (2011): 2691

In vivo detection of embryonic stem cell-derived cardiovascular progenitor cells using Cy3-labeled Gadofluorine M in murine myocardium

Authors:

Adler ED, Bystrup A, Briley-Saebo KC, Mani V, Young W, Giovanonne S, Altman P, Kattman SJ, Frank JA, Weinmann HJ, Keller GM, Fayad ZA.

Journal:

JACC Cardiovasc Imaging (2009): 1114

Quantitative proteomics by fluorescent labeling of cysteine residues using a set of two cyanine-based or three rhodamine-based dyes

Authors:

Volke D, Hoffmann R.

Journal:

Electrophoresis (2008): 4516

Physical properties

Molecular weight	730.33
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.05
Correction factor (280 nm)	0.101
Extinction coefficient (cm ^-1 M ^-1)	250000
Excitation (nm)	682
Emission (nm)	702
Quantum yield	0.27

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 12, 2025