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Cy3.5 tetrazine
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1377.80 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.08 |
| Correction Factor (280 nm) | 0.178 |
| Extinction coefficient (cm -1 M -1) | 150000 |
| Excitation (nm) | 579 |
| Emission (nm) | 591 |
| Quantum yield | 0.15 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
| Overview |  SDSProtocol |
Molecular weight 1377.80 | Correction Factor (260 nm) 0.08 | Correction Factor (280 nm) 0.178 | Extinction coefficient (cm -1 M -1) 150000 | Excitation (nm) 579 | Emission (nm) 591 | Quantum yield 0.15 |
Cy3.5 tetrazine is an excellent building block that can be readily used to label TCO-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. It is widely used for labeling TCO-modified peptides, proteins and oligos etc. The inverse electron demand Diels–Alder (IEDDA) between 1,2,4,5-tetrazine and strained alkene (such as trans-cyclooctene, TCO) is a well-established bioorthogonal reaction. It is generally considered to be the fastest click reaction with first-order rate constants ranging up to 100,000 M−1 S−1. Since the first report on IEDDA reaction, several kinds of strained alkenes/alkynes and tetrazine analogs have been synthesized, and these functional group pairs have been applied to life science research and nanomaterials. Due to the extremely rapid reaction rate of IEDDA under mild conditions such as room temperature, neutral pH, and in aqueous media, this reaction has been a highly useful ligation approach for a variety of bioconjugations. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3.5 tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 72.579 µL | 362.897 µL | 725.795 µL | 3.629 mL | 7.258 mL |
| 5 mM | 14.516 µL | 72.579 µL | 145.159 µL | 725.795 µL | 1.452 mL |
| 10 mM | 7.258 µL | 36.29 µL | 72.579 µL | 362.897 µL | 725.795 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.08 |
| Correction Factor (280 nm) | 0.178 |
| Extinction coefficient (cm -1 M -1) | 150000 |
| Excitation (nm) | 579 |
| Emission (nm) | 591 |
| Quantum yield | 0.15 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (482 nm) | Correction Factor (565 nm) | Correction Factor (650 nm) |
| Cy5.5 tetrazine | 683 | 703 | 250000 | 0.27 | 0.05 | 0.101 | 0.0017 | 0.047 | 0.454 |
Images
Figure 1. Cy3.5 tetrazine is an excellent building block that can be readily used to label TCO-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. The inverse electron demand Diels–Alder (IEDDA) between 1,2,4,5-tetrazine and strained alkene (such as trans-cyclooctene, TCO) is a well-established bioorthogonal reaction. It is generally considered to be the fastest click reaction with first-order rate constants ranging up to 100,000 M−1 S−1.
References
View all 7 references: Citation Explorer
Evaluation of bioorthogonally applicable tetrazine-Cy3 probes for fluorogenic labeling schemes.
Authors: Albitz, Evelin and Németh, Krisztina and Knorr, Gergely and Kele, Péter
Journal: Organic & biomolecular chemistry (2023): 7358-7366
Authors: Albitz, Evelin and Németh, Krisztina and Knorr, Gergely and Kele, Péter
Journal: Organic & biomolecular chemistry (2023): 7358-7366
Analysis of protein phosphorylation in solution and in cells by using an ATP analogue in combination with fluorescence techniques.
Authors: Li, Yue and Huang, Xiangyi and Ren, Jicun
Journal: The Analyst (2021): 4506-4514
Authors: Li, Yue and Huang, Xiangyi and Ren, Jicun
Journal: The Analyst (2021): 4506-4514
Design, Synthesis, Conjugation, and Reactivity of Novel trans,trans-1,5-Cyclooctadiene-Derived Bioorthogonal Linkers.
Authors: Longo, Beatrice and Zanato, Chiara and Piras, Monica and Dall'Angelo, Sergio and Windhorst, Albert D and Vugts, Danielle J and Baldassarre, Massimiliano and Zanda, Matteo
Journal: Bioconjugate chemistry (2020)
Authors: Longo, Beatrice and Zanato, Chiara and Piras, Monica and Dall'Angelo, Sergio and Windhorst, Albert D and Vugts, Danielle J and Baldassarre, Massimiliano and Zanda, Matteo
Journal: Bioconjugate chemistry (2020)
A rapid and concise setup for the fast screening of FRET pairs using bioorthogonalized fluorescent dyes.
Authors: Petrovics, Réka and Söveges, Bianka and Egyed, Alexandra and Knorr, Gergely and Kormos, Attila and Imre, Tímea and Török, György and Zeke, András and Kocsmár, Éva and Lotz, Gábor and Kele, Péter and Németh, Krisztina
Journal: Organic & biomolecular chemistry (2018): 2997-3005
Authors: Petrovics, Réka and Söveges, Bianka and Egyed, Alexandra and Knorr, Gergely and Kormos, Attila and Imre, Tímea and Török, György and Zeke, András and Kocsmár, Éva and Lotz, Gábor and Kele, Péter and Németh, Krisztina
Journal: Organic & biomolecular chemistry (2018): 2997-3005
Bioorthogonally Applicable Fluorogenic Cyanine-Tetrazines for No-Wash Super-Resolution Imaging.
Authors: Knorr, Gergely and Kozma, Eszter and Schaart, Judith M and Németh, Krisztina and Török, György and Kele, Péter
Journal: Bioconjugate chemistry (2018): 1312-1318
Authors: Knorr, Gergely and Kozma, Eszter and Schaart, Judith M and Németh, Krisztina and Török, György and Kele, Péter
Journal: Bioconjugate chemistry (2018): 1312-1318
Azide and trans-cyclooctene dUTPs: incorporation into DNA probes and fluorescent click-labelling.
Authors: Ren, Xiaomei and El-Sagheer, Afaf H and Brown, Tom
Journal: The Analyst (2015): 2671-8
Authors: Ren, Xiaomei and El-Sagheer, Afaf H and Brown, Tom
Journal: The Analyst (2015): 2671-8
Rapid labeling of metabolically engineered cell-surface glycoconjugates with a carbamate-linked cyclopropene reporter.
Authors: Späte, Anne-Katrin and Bußkamp, Holger and Niederwieser, Andrea and Schart, Verena F and Marx, Andreas and Wittmann, Valentin
Journal: Bioconjugate chemistry (2014): 147-54
Authors: Späte, Anne-Katrin and Bußkamp, Holger and Niederwieser, Andrea and Schart, Verena F and Marx, Andreas and Wittmann, Valentin
Journal: Bioconjugate chemistry (2014): 147-54