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AAT Bioquest

Cy5.5 tetrazine

Cy5.5 tetrazine is an excellent building block that can be readily used to label TCO-modified biological molecules for NIR fluorescence imaging and other fluorescence-based biochemical analysis. It is widely used for labeling TCO-modified peptides, proteins and oligos etc. The inverse electron demand Diels–Alder (IEDDA) between 1,2,4,5-tetrazine and strained alkene (such as trans-cyclooctene, TCO) is a well-established bioorthogonal reaction. It is generally considered to be the fastest click reaction with first-order rate constants ranging up to 100,000 M−1 S−1. Since the first report on IEDDA reaction, several kinds of strained alkenes/alkynes and tetrazine analogs have been synthesized, and these functional group pairs have been applied to drug discovery, life science research and nanomaterials. Due to the extremely rapid reaction rate of IEDDA under mild conditions such as room temperature, neutral pH, and in aqueous media, this reaction has been a highly useful ligation approach for a variety of bioconjugations. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Cy3.5 tetrazine5795911500000.150.080.178

References

View all 8 references: Citation Explorer
Optimization of IEDDA bioorthogonal system: Efficient process to improve trans-cyclooctene/tetrazine interaction.
Authors: Béquignat, Jean-Baptiste and Ty, Nancy and Rondon, Aurélie and Taiariol, Ludivine and Degoul, Françoise and Canitrot, Damien and Quintana, Mercedes and Navarro-Teulon, Isabelle and Miot-Noirault, Elisabeth and Boucheix, Claude and Chezal, Jean-Michel and Moreau, Emmanuel
Journal: European journal of medicinal chemistry (2020): 112574
Labeling a TCO-functionalized single domain antibody fragment with 18F via inverse electron demand Diels Alder cycloaddition using a fluoronicotinyl moiety-bearing tetrazine derivative.
Authors: Zhou, Zhengyuan and Zalutsky, Michael R and Vaidyanathan, Ganesan
Journal: Bioorganic & medicinal chemistry (2020): 115634
Tetrazine-TCO Ligation: A Potential Simple Approach to Improve Tumor Uptake through Enhanced Blood Circulation.
Authors: Wang, Mengzhe and Wang, Hui and Niu, Crystal Q and Zhang, Tao and Wu, Zhanhong and Li, Zibo
Journal: Bioconjugate chemistry (2020): 1795-1803
Development of a self-immolative linker for tetrazine-triggered release of alcohols in cells.
Authors: Davies, Sarah and Oliveira, Bruno L and Bernardes, Gonçalo J L
Journal: Organic & biomolecular chemistry (2019): 5725-5730
An Efficient Method for Labeling Single Domain Antibody Fragments with 18F Using Tetrazine- Trans-Cyclooctene Ligation and a Renal Brush Border Enzyme-Cleavable Linker.
Authors: Zhou, Zhengyuan and Devoogdt, Nick and Zalutsky, Michael R and Vaidyanathan, Ganesan
Journal: Bioconjugate chemistry (2018): 4090-4103
Page updated on December 6, 2024

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Catalog Number904
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Physical properties

Molecular weight

1403.84

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.05

Correction Factor (280 nm)

0.101

Correction Factor (482 nm)

0.0017

Correction Factor (565 nm)

0.047

Correction Factor (650 nm)

0.454

Extinction coefficient (cm -1 M -1)

250000

Excitation (nm)

683

Emission (nm)

703

Quantum yield

0.27

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
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