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Cy5.5 tetrazine
Ordering information
| Price | |
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1403.84 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.05 |
| Correction Factor (280 nm) | 0.101 |
| Correction Factor (482 nm) | 0.0017 |
| Correction Factor (565 nm) | 0.047 |
| Correction Factor (650 nm) | 0.454 |
| Extinction coefficient (cm -1 M -1) | 250000 |
| Excitation (nm) | 683 |
| Emission (nm) | 703 |
| Quantum yield | 0.27 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| Overview |  SDSProtocol |
Molecular weight 1403.84 | Correction Factor (260 nm) 0.05 | Correction Factor (280 nm) 0.101 | Correction Factor (482 nm) 0.0017 | Correction Factor (565 nm) 0.047 | Correction Factor (650 nm) 0.454 | Extinction coefficient (cm -1 M -1) 250000 | Excitation (nm) 683 | Emission (nm) 703 | Quantum yield 0.27 |
Cy5.5 tetrazine is an excellent building block that can be readily used to label TCO-modified biological molecules for NIR fluorescence imaging and other fluorescence-based biochemical analysis. It is widely used for labeling TCO-modified peptides, proteins and oligos etc. The inverse electron demand Diels–Alder (IEDDA) between 1,2,4,5-tetrazine and strained alkene (such as trans-cyclooctene, TCO) is a well-established bioorthogonal reaction. It is generally considered to be the fastest click reaction with first-order rate constants ranging up to 100,000 M−1 S−1. Since the first report on IEDDA reaction, several kinds of strained alkenes/alkynes and tetrazine analogs have been synthesized, and these functional group pairs have been applied to drug discovery, life science research and nanomaterials. Due to the extremely rapid reaction rate of IEDDA under mild conditions such as room temperature, neutral pH, and in aqueous media, this reaction has been a highly useful ligation approach for a variety of bioconjugations. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cy5.5 tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 71.233 µL | 356.166 µL | 712.332 µL | 3.562 mL | 7.123 mL |
| 5 mM | 14.247 µL | 71.233 µL | 142.466 µL | 712.332 µL | 1.425 mL |
| 10 mM | 7.123 µL | 35.617 µL | 71.233 µL | 356.166 µL | 712.332 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.05 |
| Correction Factor (280 nm) | 0.101 |
| Correction Factor (482 nm) | 0.0017 |
| Correction Factor (565 nm) | 0.047 |
| Correction Factor (650 nm) | 0.454 |
| Extinction coefficient (cm -1 M -1) | 250000 |
| Excitation (nm) | 683 |
| Emission (nm) | 703 |
| Quantum yield | 0.27 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Cy3.5 tetrazine | 579 | 591 | 150000 | 0.15 | 0.08 | 0.178 |
Images
Figure 1. Cy5.5 tetrazine is an excellent building block that can be readily used to label TCO-modified biological molecules for NIR fluorescence imaging and other fluorescence-based biochemical analysis. The inverse electron demand Diels–Alder (IEDDA) between 1,2,4,5-tetrazine and strained alkene (such as trans-cyclooctene, TCO) is a well-established bioorthogonal reaction.
References
View all 8 references: Citation Explorer
Tetrazine-TCO Ligation: A Potential Simple Approach to Improve Tumor Uptake through Enhanced Blood Circulation.
Authors: Wang, Mengzhe and Wang, Hui and Niu, Crystal Q and Zhang, Tao and Wu, Zhanhong and Li, Zibo
Journal: Bioconjugate chemistry (2020): 1795-1803
Authors: Wang, Mengzhe and Wang, Hui and Niu, Crystal Q and Zhang, Tao and Wu, Zhanhong and Li, Zibo
Journal: Bioconjugate chemistry (2020): 1795-1803
Labeling a TCO-functionalized single domain antibody fragment with 18F via inverse electron demand Diels Alder cycloaddition using a fluoronicotinyl moiety-bearing tetrazine derivative.
Authors: Zhou, Zhengyuan and Zalutsky, Michael R and Vaidyanathan, Ganesan
Journal: Bioorganic & medicinal chemistry (2020): 115634
Authors: Zhou, Zhengyuan and Zalutsky, Michael R and Vaidyanathan, Ganesan
Journal: Bioorganic & medicinal chemistry (2020): 115634
Optimization of IEDDA bioorthogonal system: Efficient process to improve trans-cyclooctene/tetrazine interaction.
Authors: Béquignat, Jean-Baptiste and Ty, Nancy and Rondon, Aurélie and Taiariol, Ludivine and Degoul, Françoise and Canitrot, Damien and Quintana, Mercedes and Navarro-Teulon, Isabelle and Miot-Noirault, Elisabeth and Boucheix, Claude and Chezal, Jean-Michel and Moreau, Emmanuel
Journal: European journal of medicinal chemistry (2020): 112574
Authors: Béquignat, Jean-Baptiste and Ty, Nancy and Rondon, Aurélie and Taiariol, Ludivine and Degoul, Françoise and Canitrot, Damien and Quintana, Mercedes and Navarro-Teulon, Isabelle and Miot-Noirault, Elisabeth and Boucheix, Claude and Chezal, Jean-Michel and Moreau, Emmanuel
Journal: European journal of medicinal chemistry (2020): 112574
Development of a self-immolative linker for tetrazine-triggered release of alcohols in cells.
Authors: Davies, Sarah and Oliveira, Bruno L and Bernardes, Gonçalo J L
Journal: Organic & biomolecular chemistry (2019): 5725-5730
Authors: Davies, Sarah and Oliveira, Bruno L and Bernardes, Gonçalo J L
Journal: Organic & biomolecular chemistry (2019): 5725-5730
An Efficient Method for Labeling Single Domain Antibody Fragments with 18F Using Tetrazine- Trans-Cyclooctene Ligation and a Renal Brush Border Enzyme-Cleavable Linker.
Authors: Zhou, Zhengyuan and Devoogdt, Nick and Zalutsky, Michael R and Vaidyanathan, Ganesan
Journal: Bioconjugate chemistry (2018): 4090-4103
Authors: Zhou, Zhengyuan and Devoogdt, Nick and Zalutsky, Michael R and Vaidyanathan, Ganesan
Journal: Bioconjugate chemistry (2018): 4090-4103
A Versatile Microarray Immobilization Strategy Based on a Biorthogonal Reaction Between Tetrazine and Trans-Cyclooctene.
Authors: Wang, Ping and Gao, Liqian and Lei, Haipeng and Lee, Su Seong and Yao, Shao Q and Sun, Hongyan
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 67-80
Authors: Wang, Ping and Gao, Liqian and Lei, Haipeng and Lee, Su Seong and Yao, Shao Q and Sun, Hongyan
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 67-80
Multiplex imaging and cellular target identification of kinase inhibitors via an affinity-based proteome profiling approach.
Authors: Su, Ying and Pan, Sijun and Li, Zhengqiu and Li, Lin and Wu, Xiaoyuan and Hao, Piliang and Sze, Siu Kwan and Yao, Shao Q
Journal: Scientific reports (2015): 7724
Authors: Su, Ying and Pan, Sijun and Li, Zhengqiu and Li, Lin and Wu, Xiaoyuan and Hao, Piliang and Sze, Siu Kwan and Yao, Shao Q
Journal: Scientific reports (2015): 7724
Microarray immobilization of biomolecules using a fast trans-cyclooctene (TCO)-tetrazine reaction.
Authors: Wang, Ping and Na, Zhenkun and Fu, Jiaqi and Tan, Chelsea Y J and Zhang, Huatang and Yao, Shao Q and Sun, Hongyan
Journal: Chemical communications (Cambridge, England) (2014): 11818-21
Authors: Wang, Ping and Na, Zhenkun and Fu, Jiaqi and Tan, Chelsea Y J and Zhang, Huatang and Yao, Shao Q and Sun, Hongyan
Journal: Chemical communications (Cambridge, England) (2014): 11818-21