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AAT Bioquest

Cy5NS acid

Cyanines are used in a wide variety of biological applications including comparative genomic hybridization and in gene chips. Depending on the structure, they cover the spectrum visible and IR wavelengths. Cy 3, Cy5 and Cy7 are the most popular cyanine dyes, in particular Cy3 and Cy5 are typically combined for 2 color detection. Cy3 dyes fluoresce yellow-green, while Cy5 is fluorescent in the red region. They are usually synthesized with reactive groups on either one or both of the nitrogen side chains so that they can be chemically linked to either nucleic acids or protein molecules. AAT Bioquest offers the broadest selection of cyanine dyes. This non-sulfonated cyanine is not water soluble, and mainly used for labeling oligos, peptides and other small molecules. Cy5NS has chemical and spectral properties identical to Quasar 670 (Biosearch).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy5NS acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM207.185 µL1.036 mL2.072 mL10.359 mL20.719 mL
5 mM41.437 µL207.185 µL414.37 µL2.072 mL4.144 mL
10 mM20.719 µL103.593 µL207.185 µL1.036 mL2.072 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)Correction Factor (260 nm)
ICG acid789813230000-0.076-
Cy3NS acid *CAS 1032678-01-5*55556915000010.1510.0730.07
Cy7NS acid7567792500000.30.0360.05
AMCA acid *CAS#: 106562-32-7*34643419000-0.153-
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] *CAS 50402-56-7*3364555900-0.107-
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]3364555900-0.107-
DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1*----0.516-
BCECF acid *CAS#: 85138-49-4*50452790000---
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid*534553810000.6110.090.24
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid*499520710000.9210.110.30
Cy3B acid56057112000010.5810.0690.048
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid*34344119000-0.190.25
XFD546 acid *Same Structure to Alexa Fluor™ 546 acid*5615721120000.7910.120.21
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid*579603913000.6910.460.45
XFD514 acid51854380000-0.180.31
QXY7 acid [equivalent to QSY-7 acid]--900001-0.22-
XFD555 acid5535681550000.110.080.08
XFD647 acid6506712700000.3310.03-
XFD750 acid7527762900000.1210.04-
XFD700 acid6967192050000.2510.07-
OG514 acid [equivalent to Oregon Green® 514 carboxylic acid]51353385000-0.19-
XFD405 acid40142135,000-0.700.23
XFD430 acid43254015,000-0.28-
XFD594 acid590618900000.6610.560.43
Show More (15)

Citations

View all 13 citations: Citation Explorer
Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization
Authors: Wang, Xiaoyan and Zhang, Yu and Xue, Wei and Wang, Hong and Qiu, Xiaozhong and Liu, Zonghua
Journal: Journal of Biomaterials Applications (2017): 923--932
Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao
Journal: Colloids and Surfaces B: Biointerfaces (2017)
Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics
Authors: Guo, Yuxin and Zhang, Yang and Ma, Jinyuan and Li, Qi and Li, Yang and Zhou, Xinyi and Zhao, Dan and Song, Hua and Chen, Qing and Zhu, Xuan
Journal: Journal of Controlled Release (2017)
Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction
Authors: Liu, Cuiping and Stonestrom, Aaron J and Christian, Thomas and Yong, Jeongsik and Takase, Ryuichi and Hou, Ya-Ming and Yang, Xiaolu
Journal: Journal of Biological Chemistry (2016): 10426--10436
Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules
Authors: Ngo, John T and Adams, Stephen R and Deerinck, Thomas J and Boassa, Daniela and Rodriguez-Rivera, Frances and Palida, Sakina F and Bertozzi, Carolyn R and Ellisman, Mark H and Tsien, Roger Y
Journal: Nat Chem Biol (2016): 459--465

References

View all 21 references: Citation Explorer
Excitation of Cy5 in self-assembled lipid bilayers using optical microresonators
Authors: Freeman LM, Li S, Dayani Y, Choi HS, Malmstadt N, Armani AM.
Journal: Appl Phys Lett (2011): 143703
Theranostic cRGD-BioShuttle Constructs Containing Temozolomide- and Cy7 For NIR-Imaging and Therapy
Authors: Wiessler M, Hennrich U, Pipkorn R, Waldeck W, Cao L, Peter J, Ehemann V, Semmler W, Lammers T, Braun K.
Journal: Theranostics (2011): 381
Rational approach to select small peptide molecular probes labeled with fluorescent cyanine dyes for in vivo optical imaging
Authors: Berezin MY, Guo K, Akers W, Livingston J, Solomon M, Lee H, Liang K, Agee A, Achilefu S.
Journal: Biochemistry (2011): 2691
In vivo detection of embryonic stem cell-derived cardiovascular progenitor cells using Cy3-labeled Gadofluorine M in murine myocardium
Authors: Adler ED, Bystrup A, Briley-Saebo KC, Mani V, Young W, Giovanonne S, Altman P, Kattman SJ, Frank JA, Weinmann HJ, Keller GM, Fayad ZA.
Journal: JACC Cardiovasc Imaging (2009): 1114
Quantitative proteomics by fluorescent labeling of cysteine residues using a set of two cyanine-based or three rhodamine-based dyes
Authors: Volke D, Hoffmann R.
Journal: Electrophoresis (2008): 4516
Page updated on October 11, 2024

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Physical properties

Molecular weight

482.66

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.02

Correction Factor (280 nm)

0.03

Correction Factor (482 nm)

0.009

Correction Factor (565 nm)

0.09

Extinction coefficient (cm -1 M -1)

2500001

Excitation (nm)

651

Emission (nm)

670

Quantum yield

0.271, 0.42

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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