Cy7 tyramide

Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. The signal amplification conferred by the turnover of multiple tyramide substrates per peroxidase label translates ultrasensitive detection of low-abundance targets and the use of smaller amounts of antibodies and hybridization probes. In immunohistochemical applications, sensitivity enhancements derived from TSA method allow primary antibody dilutions to be increased to reduce nonspecific background signals, and can overcome weak immunolabeling caused by suboptimal fixation procedures or low levels of target expression. Cy7 tyramide contains the popular Cy7 fluorophore that can be readily detected with the standard Cy7 filter set. Its NIR excitation and emission make the probe an ideal choice for the applications where the common tyramides may have an interference resulted from the inherent fluorescence of tissues or other samples.

Protocol

SDS

Catalog	Size	Price	Quantity
11064	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy7 tyramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	110.716 µL	553.581 µL	1.107 mL	5.536 mL	11.072 mL
5 mM	22.143 µL	110.716 µL	221.432 µL	1.107 mL	2.214 mL
10 mM	11.072 µL	55.358 µL	110.716 µL	553.581 µL	1.107 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	903.21 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Tyramide Signal-Amplified Immunofluorescence of MYCN and MYC in Human Tissue Specimens and Cell Line Cultures.

Authors:

Schafer, Johanna M and Pietenpol, Jennifer A

Journal:

Bio-protocol (2020): e3677

Cascade signal amplification for sensitive detection of exosomes by integrating tyramide and surface-initiated enzymatic polymerization.

Authors:

Huang, Zhipeng and Lin, Qiuyuan and Yang, Bin and Ye, Xin and Chen, Hui and Weng, Wenhao and Kong, Jilie

Journal:

Chemical communications (Cambridge, England) (2020): 12793-12796

Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence.

Authors:

Wang, Herui and Pangilinan, Ryan L and Zhu, Yan

Journal:

Methods in molecular biology (Clifton, N.J.) (2020): 89-97

Characterizing the Tumor Immune Microenvironment with Tyramide-Based Multiplex Immunofluorescence.

Authors:

Mori, Hidetoshi and Bolen, Jennifer and Schuetter, Louis and Massion, Pierre and Hoyt, Clifford C and VandenBerg, Scott and Esserman, Laura and Borowsky, Alexander D and Campbell, Michael J

Journal:

Journal of mammary gland biology and neoplasia (2020): 417-432

Procedural Requirements and Recommendations for Multiplex Immunofluorescence Tyramide Signal Amplification Assays to Support Translational Oncology Studies.

Authors:

Parra, Edwin Roger and Jiang, Mei and Solis, Luisa and Mino, Barbara and Laberiano, Caddie and Hernandez, Sharia and Gite, Swati and Verma, Anuj and Tetzlaff, Michael and Haymaker, Cara and Tamegnon, Auriole and Rodriguez-Canales, Jaime and Hoyd, Clifford and Bernachez, Chantale and Wistuba, Ignacio

Journal:

Cancers (2020)

Physical properties

Molecular weight	903.21
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.05
Correction factor (280 nm)	0.036
Correction factor (482 nm)	0.0005
Correction factor (565 nm)	0.0193
Correction factor (650 nm)	0.165
Extinction coefficient (cm ^-1 M ^-1)	250000
Excitation (nm)	756
Emission (nm)	779
Quantum yield	0.3

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	Cy7 filter set
Emission	Cy7 filter set
Recommended plate	Black wall/clear bottom

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Power Styramide™ Signal Amplification (PSA™)

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on November 20, 2025