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Cy7®-streptavidin conjugate

Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This Cy7-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with Cy7 covalently attached (as the fluorescent label). Our Cy7-streptavidin conjugate was prepared using AAT Bioquest's proprietary labeling technology. It demonstrated much brighter signal compared to the similar Cy7-streptavidin conjugates from other commercial sources, thus can significantly increase assay sensitivities. It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Cy7 filter set. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Cy7 biotin conjugate7567792500000.30.050.036
RPE-streptavidin conjugate56557419600000.82-0.175
APC-streptavidin conjugate651660730000--0.195
PerCP-streptavidin conjugate477678406000--0.22
FITC-streptavidin conjugate491516730000.92-0.35
Cy3®-streptavidin conjugate55556915000010.1510.070.073
Cy5®-streptavidin conjugate65167025000010.271, 0.420.020.03
XFD350-streptavidin conjugate *XFD350 Same Structure to Alexa Fluor™ 350*34344119000-0.250.19
XFD488-streptavidin conjugate *XFD488 Same Structure to Alexa Fluor™ 488*499520730000.9210.30.11
XFD594-streptavidin conjugate *XFD594 Same Structure to Alexa Fluor™ 594*590618920000.6610.430.56
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Citations

View all 3 citations: Citation Explorer
Persistent repression of tau in the brain using engineered zinc finger protein transcription factors
Authors: Wegmann, Susanne and DeVos, Sarah L and Zeitler, Bryan and Marlen, Kimberly and Bennett, Rachel E and Perez-Rando, Marta and MacKenzie, Danny and Yu, Qi and Commins, Caitlin and Bannon, Riley N and others,
Journal: Science advances (2021): eabe1611
Persistent repression of tau in the brain using engineered zinc finger protein transcription factors
Authors: Wegmann, Susanne and DeVos, Sarah L and Zeitler, Bryan and Marlen, Kimberly and Bennett, Rachel E and Perez-Rando, Marta and MacKenzie, Danny and Yu, Qi and Commins, Caitlin and Bannon, Riley N and others,
Journal: Science Advances (2021): eabe1611
Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996

References

View all 47 references: Citation Explorer
A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647
Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50
Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label
Authors: Sun M, Du L, Gao S, Bao Y, Wang S.
Journal: Steroids (2010): 400
Page updated on December 11, 2024

Ordering information

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Catalog Number16914
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Physical properties

Molecular weight

~52000

Solvent

Water

Spectral properties

Correction Factor (260 nm)

0.05

Correction Factor (280 nm)

0.036

Correction Factor (482 nm)

0.0005

Correction Factor (565 nm)

0.0193

Correction Factor (650 nm)

0.165

Extinction coefficient (cm -1 M -1)

250000

Excitation (nm)

756

Emission (nm)

779

Quantum yield

0.3

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by Cy7&reg;-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel.
HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by Cy7&reg;-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel.
HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by Cy7&reg;-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel.