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Cy7®-streptavidin conjugate
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This Cy7-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with Cy7 covalently attached (as the fluorescent label). Our Cy7-streptavidin conjugate was prepared using AAT Bioquest's proprietary labeling technology. It demonstrated much brighter signal compared to the similar Cy7-streptavidin conjugates from other commercial sources, thus can significantly increase assay sensitivities. It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Cy7 filter set. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.
<p>HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by Cy7&reg;-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel.</p>
<p>HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by Cy7&reg;-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
169141 mg
Price
 
Physical properties
Molecular weight~52000
SolventWater
Spectral properties
Correction factor (260 nm)0.05
Correction factor (280 nm)0.036
Correction factor (482 nm)0.0005
Correction factor (565 nm)0.0193
Correction factor (650 nm)0.165
Extinction coefficient (cm -1 M -1)
250000
Excitation (nm)756
Emission (nm)779
Quantum yield
0.3
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 8, 2025