Calculator
Cy7.5 tetrazine
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1429.88 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
| Overview |  SDSProtocol |
See also: Cyanines
Molecular weight 1429.88 |
Cy7.5 tetrazine is a conjugate formed by attaching a tetrazine molecule to the Cy7.5 fluorophore. This conjugate is particularly valuable in bioorthogonal labeling strategies, where it can react selectively and rapidly with a complementary molecule containing TCO, a strained alkene (typically trans-cyclooctene) in a reaction known as the inverse electron-demand Diels-Alder (IEDDA) reaction. The IEDDA reaction between Cy7.5 tetrazine and a strained alkene group allows for specific and efficient labeling of biomolecules without interfering with native biological processes. This labeling method has gained popularity in biochemistry, cell biology, and imaging studies due to its bioorthogonal nature and compatibility with living systems. Cy7.5 tetrazine can provide a valuable infrared fluorescent tool for labeling and tracking specific molecules or structures of interest. Its IR fluorescence properties also make it suitable for deep tissue imaging applications.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cy7.5 tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 69.936 µL | 349.68 µL | 699.359 µL | 3.497 mL | 6.994 mL |
| 5 mM | 13.987 µL | 69.936 µL | 139.872 µL | 699.359 µL | 1.399 mL |
| 10 mM | 6.994 µL | 34.968 µL | 69.936 µL | 349.68 µL | 699.359 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (482 nm) | Correction Factor (565 nm) | Correction Factor (650 nm) |
| Cy3.5 tetrazine | 579 | 591 | 150000 | 0.15 | 0.08 | 0.178 | - | - | - |
| Cy5.5 tetrazine | 683 | 703 | 250000 | 0.27 | 0.05 | 0.101 | 0.0017 | 0.047 | 0.454 |
Images
Figure 1. Cy7.5 tetrazine is a conjugate formed by attaching a tetrazine molecule to the Cy7.5 fluorophore. This conjugate is particularly valuable in biorthogonal labeling strategies, where it can react selectively and rapidly with a complementary molecule containing TCO, a strained alkene (typically trans-cyclooctene) in a reaction known as the inverse electron-demand Diels-Alder (IEDDA) reaction.
References
View all 50 references: Citation Explorer
Acidic pH-Triggered Live-Cell Lysosome Specific Tracking, Ratiometric pH Sensing, and Multicolor Imaging by Visible to NIR Switchable Cy-7 Dyes.
Authors: Mukherjee, Ayan and Saha, Pranab Chandra and Kar, Samiran and Guha, Pampa and Das, Rabi Sankar and Bera, Tapas and Guha, Samit
Journal: Chembiochem : a European journal of chemical biology (2023): e202200641
Authors: Mukherjee, Ayan and Saha, Pranab Chandra and Kar, Samiran and Guha, Pampa and Das, Rabi Sankar and Bera, Tapas and Guha, Samit
Journal: Chembiochem : a European journal of chemical biology (2023): e202200641
Boosting Dye-Sensitized Luminescence by Enhanced Short-Range Triplet Energy Transfer.
Authors: Zhao, Fei and Hu, Jialing and Guan, Daoming and Liu, Jinyang and Zhang, Xuebo and Ling, Huan and Zhang, Yunxiang and Liu, Qian
Journal: Advanced materials (Deerfield Beach, Fla.) (2023): e2304907
Authors: Zhao, Fei and Hu, Jialing and Guan, Daoming and Liu, Jinyang and Zhang, Xuebo and Ling, Huan and Zhang, Yunxiang and Liu, Qian
Journal: Advanced materials (Deerfield Beach, Fla.) (2023): e2304907
Comparison of Five Near-Infrared Fluorescent Folate Conjugates in an Ovarian Cancer Model.
Authors: García de Jalón, Elvira and Kleinmanns, Katrin and Fosse, Vibeke and Davidson, Ben and Bjørge, Line and Haug, Bengt Erik and McCormack, Emmet
Journal: Molecular imaging and biology (2023): 144-155
Authors: García de Jalón, Elvira and Kleinmanns, Katrin and Fosse, Vibeke and Davidson, Ben and Bjørge, Line and Haug, Bengt Erik and McCormack, Emmet
Journal: Molecular imaging and biology (2023): 144-155
Polymer Vesicles with Integrated Photothermal Responsiveness.
Authors: Luo, Yingtong and Wu, Hanglong and Zhou, Xuan and Wang, Jianhong and Er, Süleyman and Li, Yudong and Welzen, Pascal L W and Oerlemans, Roy A J F and Abdelmohsen, Loai K E A and Shao, Jingxin and van Hest, Jan C M
Journal: Journal of the American Chemical Society (2023): 20073-20080
Authors: Luo, Yingtong and Wu, Hanglong and Zhou, Xuan and Wang, Jianhong and Er, Süleyman and Li, Yudong and Welzen, Pascal L W and Oerlemans, Roy A J F and Abdelmohsen, Loai K E A and Shao, Jingxin and van Hest, Jan C M
Journal: Journal of the American Chemical Society (2023): 20073-20080
Stepwise Energy Transfer: Near-Infrared Persistent Luminescence from Doped Polymeric Systems.
Authors: Lin, Faxu and Wang, Haiyang and Cao, Yifeng and Yu, Rujun and Liang, Guodong and Huang, Huahua and Mu, Yingxiao and Yang, Zhiyong and Chi, Zhenguo
Journal: Advanced materials (Deerfield Beach, Fla.) (2022): e2108333
Authors: Lin, Faxu and Wang, Haiyang and Cao, Yifeng and Yu, Rujun and Liang, Guodong and Huang, Huahua and Mu, Yingxiao and Yang, Zhiyong and Chi, Zhenguo
Journal: Advanced materials (Deerfield Beach, Fla.) (2022): e2108333
Reducing thermal damage to adjacent normal tissue with dual thermo-responsive polymer via thermo-induced phase transition for precise photothermal theranosis.
Authors: Wang, Rui and Wang, Xu and Mu, Xueluer and Feng, Wenbi and Lu, Yingxi and Yu, Weisong and Zhou, Xianfeng
Journal: Acta biomaterialia (2022): 142-151
Authors: Wang, Rui and Wang, Xu and Mu, Xueluer and Feng, Wenbi and Lu, Yingxi and Yu, Weisong and Zhou, Xianfeng
Journal: Acta biomaterialia (2022): 142-151
A Raman/fluorescence dual-modal imaging guided synergistic photothermal and photodynamic therapy nanoplatform for precision cancer theranostics.
Authors: Fu, Lili and Huang, Yan and Hou, Junjun and Sun, Mingzhao and Wang, Lingxiao and Wang, Xiaoyan and Chen, Lingxin
Journal: Journal of materials chemistry. B (2022): 8432-8442
Authors: Fu, Lili and Huang, Yan and Hou, Junjun and Sun, Mingzhao and Wang, Lingxiao and Wang, Xiaoyan and Chen, Lingxin
Journal: Journal of materials chemistry. B (2022): 8432-8442
Fluorescent A2A and A3 adenosine receptor antagonists as flow cytometry probes.
Authors: Toti, Kiran S and Campbell, Ryan G and Lee, Hobin and Salmaso, Veronica and Suresh, R Rama and Gao, Zhan-Guo and Jacobson, Kenneth A
Journal: Purinergic signalling (2022)
Authors: Toti, Kiran S and Campbell, Ryan G and Lee, Hobin and Salmaso, Veronica and Suresh, R Rama and Gao, Zhan-Guo and Jacobson, Kenneth A
Journal: Purinergic signalling (2022)
Computational Design, Synthesis, and Photochemistry of Cy7-PPG, an Efficient NIR-Activated Photolabile Protecting Group for Therapeutic Applications.
Authors: Alachouzos, Georgios and Schulte, Albert M and Mondal, Anirban and Szymanski, Wiktor and Feringa, Ben L
Journal: Angewandte Chemie (International ed. in English) (2022): e202201308
Authors: Alachouzos, Georgios and Schulte, Albert M and Mondal, Anirban and Szymanski, Wiktor and Feringa, Ben L
Journal: Angewandte Chemie (International ed. in English) (2022): e202201308
Signal Amplification Pretargeted PET/Fluorescence Imaging Based on Human Serum Albumin-Encapsulated GdF3 Nanoparticles for Diagnosis of Ovarian Cancer.
Authors: Shi, Xudong and Gao, Kai and Zhang, Guoxin and Zhang, Wenlong and Yang, Xingjiu and Gao, Ran
Journal: ACS biomaterials science & engineering (2022): 4956-4964
Authors: Shi, Xudong and Gao, Kai and Zhang, Guoxin and Zhang, Wenlong and Yang, Xingjiu and Gao, Ran
Journal: ACS biomaterials science & engineering (2022): 4956-4964