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Cy7 biotin conjugate
Cy7 biotin conjugate demonstrates excellent avidin binding and strong NIR fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. Cy7 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and short wavelength emission. Cy7 Biotin is a water-soluble reagent and its near infrared fluorescence is pH independent from pH 4 to pH 10. A flexible PEG spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.
Chemical structure for Cy7 biotin conjugate.
Chemical structure for Cy7 biotin conjugate.
CatalogSize
Price
Quantity
31065 mg
Price
 
Physical properties

Molecular weight1291.49
SolventWater
Spectral properties

Correction factor (260 nm)0.05
Correction factor (280 nm)0.036
Correction factor (482 nm)0.0005
Correction factor (565 nm)0.0193
Correction factor (650 nm)0.165
Extinction coefficient (cm -1 M -1)
250000
Excitation (nm)756
Emission (nm)779
Quantum yield
0.3
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 13, 2025