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Cy7 biotin conjugate

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Physical properties
Molecular weight1291.49
SolventWater
Spectral properties
Correction Factor (260 nm)0.05
Correction Factor (280 nm)0.036
Correction Factor (482 nm)0.0005
Correction Factor (565 nm)0.0193
Correction Factor (650 nm)0.165
Extinction coefficient (cm -1 M -1)250000
Excitation (nm)756
Emission (nm)779
Quantum yield0.3
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


See also: Cyanines
Molecular weight
1291.49
Correction Factor (260 nm)
0.05
Correction Factor (280 nm)
0.036
Correction Factor (482 nm)
0.0005
Correction Factor (565 nm)
0.0193
Correction Factor (650 nm)
0.165
Extinction coefficient (cm -1 M -1)
250000
Excitation (nm)
756
Emission (nm)
779
Quantum yield
0.3
Cy7 biotin conjugate demonstrates excellent avidin binding and strong NIR fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. Cy7 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and short wavelength emission. Cy7 Biotin is a water-soluble reagent and its near infrared fluorescence is pH independent from pH 4 to pH 10. A flexible PEG spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cy7 biotin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM77.43 µL387.15 µL774.299 µL3.871 mL7.743 mL
5 mM15.486 µL77.43 µL154.86 µL774.299 µL1.549 mL
10 mM7.743 µL38.715 µL77.43 µL387.15 µL774.299 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.05
Correction Factor (280 nm)0.036
Correction Factor (482 nm)0.0005
Correction Factor (565 nm)0.0193
Correction Factor (650 nm)0.165
Extinction coefficient (cm -1 M -1)250000
Excitation (nm)756
Emission (nm)779
Quantum yield0.3

Images


References


View all 6 references: Citation Explorer
Multistep Consolidated Phototherapy Mediated by a NIR-Activated Photosensitizer.
Authors: Xue, Yudong and Li, Jipeng and Yang, Guoliang and Liu, Zhiyong and Zhou, Huifang and Zhang, Weian
Journal: ACS applied materials & interfaces (2019): 33628-33636
Drug-Initiated Synthesis of Heterotelechelic Polymer Prodrug Nanoparticles for in Vivo Imaging and Cancer Cell Targeting.
Authors: Vinciguerra, Daniele and Degrassi, Anna and Mancini, Laura and Mura, Simona and Mougin, Julie and Couvreur, Patrick and Nicolas, Julien
Journal: Biomacromolecules (2019): 2464-2476
Site-Specific Covalent Labeling of RNA by Enzymatic Transglycosylation.
Authors: Alexander, Seth C and Busby, Kayla N and Cole, Christian M and Zhou, Cun Yu and Devaraj, Neal K
Journal: Journal of the American Chemical Society (2015): 12756-9
Disulfide-based multifunctional conjugates for targeted theranostic drug delivery.
Authors: Lee, Min Hee and Sessler, Jonathan L and Kim, Jong Seung
Journal: Accounts of chemical research (2015): 2935-46
Allo-7: a new fluorescent tandem dye for use in flow cytometry.
Authors: Beavis, A J and Pennline, K J
Journal: Cytometry (1996): 390-5
Anomalous fluorescence enhancement of Cy3 and cy3.5 versus anomalous fluorescence loss of Cy5 and Cy7 upon covalent linking to IgG and noncovalent binding to avidin.
Authors: Gruber, H J and Hahn, C D and Kada, G and Riener, C K and Harms, G S and Ahrer, W and Dax, T G and Knaus, H G
Journal: Bioconjugate chemistry : 696-704