Cy7 biotin conjugate

Cy7 biotin conjugate demonstrates excellent avidin binding and strong NIR fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. Cy7 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and short wavelength emission. Cy7 Biotin is a water-soluble reagent and its near infrared fluorescence is pH independent from pH 4 to pH 10. A flexible PEG spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)	Correction Factor (482 nm)	Correction Factor (565 nm)	Correction Factor (650 nm)
Cy7®-streptavidin conjugate	756	779	250000	0.3	0.05	0.036	0.0005	0.0193	0.165
Cy3 biotin conjugate	555	569	150000¹	0.15¹	0.07	0.073	-	-	-

References

View all 6 references: Citation Explorer

Multistep Consolidated Phototherapy Mediated by a NIR-Activated Photosensitizer.
Authors: Xue, Yudong and Li, Jipeng and Yang, Guoliang and Liu, Zhiyong and Zhou, Huifang and Zhang, Weian
Journal: ACS applied materials & interfaces (2019): 33628-33636

Drug-Initiated Synthesis of Heterotelechelic Polymer Prodrug Nanoparticles for in Vivo Imaging and Cancer Cell Targeting.
Authors: Vinciguerra, Daniele and Degrassi, Anna and Mancini, Laura and Mura, Simona and Mougin, Julie and Couvreur, Patrick and Nicolas, Julien
Journal: Biomacromolecules (2019): 2464-2476

Site-Specific Covalent Labeling of RNA by Enzymatic Transglycosylation.
Authors: Alexander, Seth C and Busby, Kayla N and Cole, Christian M and Zhou, Cun Yu and Devaraj, Neal K
Journal: Journal of the American Chemical Society (2015): 12756-9

Disulfide-based multifunctional conjugates for targeted theranostic drug delivery.
Authors: Lee, Min Hee and Sessler, Jonathan L and Kim, Jong Seung
Journal: Accounts of chemical research (2015): 2935-46

Allo-7: a new fluorescent tandem dye for use in flow cytometry.
Authors: Beavis, A J and Pennline, K J
Journal: Cytometry (1996): 390-5

Page updated on May 19, 2025

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Catalog Number	3106
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Physical properties

Molecular weight	1291.49
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.05
Correction Factor (280 nm)	0.036
Correction Factor (482 nm)	0.0005
Correction Factor (565 nm)	0.0193
Correction Factor (650 nm)	0.165
Extinction coefficient (cm ^-1 M ^-1)	250000
Excitation (nm)	756
Emission (nm)	779
Quantum yield	0.3

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501