Cy7 biotin conjugate

Cy7 biotin conjugate demonstrates excellent avidin binding and strong NIR fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. Cy7 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and short wavelength emission. Cy7 Biotin is a water-soluble reagent and its near infrared fluorescence is pH independent from pH 4 to pH 10. A flexible PEG spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
3106	5 mg	Price

References

View all 6 references: Citation Explorer

Multistep Consolidated Phototherapy Mediated by a NIR-Activated Photosensitizer.

Authors:

Xue, Yudong and Li, Jipeng and Yang, Guoliang and Liu, Zhiyong and Zhou, Huifang and Zhang, Weian

Journal:

ACS applied materials & interfaces (2019): 33628-33636

Drug-Initiated Synthesis of Heterotelechelic Polymer Prodrug Nanoparticles for in Vivo Imaging and Cancer Cell Targeting.

Authors:

Vinciguerra, Daniele and Degrassi, Anna and Mancini, Laura and Mura, Simona and Mougin, Julie and Couvreur, Patrick and Nicolas, Julien

Journal:

Biomacromolecules (2019): 2464-2476

Site-Specific Covalent Labeling of RNA by Enzymatic Transglycosylation.

Authors:

Alexander, Seth C and Busby, Kayla N and Cole, Christian M and Zhou, Cun Yu and Devaraj, Neal K

Journal:

Journal of the American Chemical Society (2015): 12756-9

Disulfide-based multifunctional conjugates for targeted theranostic drug delivery.

Authors:

Lee, Min Hee and Sessler, Jonathan L and Kim, Jong Seung

Journal:

Accounts of chemical research (2015): 2935-46

Allo-7: a new fluorescent tandem dye for use in flow cytometry.

Authors:

Beavis, A J and Pennline, K J

Journal:

Cytometry (1996): 390-5

Physical properties

Molecular weight	1291.49
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.05
Correction factor (280 nm)	0.036
Correction factor (482 nm)	0.0005
Correction factor (565 nm)	0.0193
Correction factor (650 nm)	0.165
Extinction coefficient (cm ^-1 M ^-1)	250000
Excitation (nm)	756
Emission (nm)	779
Quantum yield	0.3

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2025