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Cy7 biotin conjugate
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1291.49 |
| Solvent | Water |
Spectral properties
| Correction Factor (260 nm) | 0.05 |
| Correction Factor (280 nm) | 0.036 |
| Correction Factor (482 nm) | 0.0005 |
| Correction Factor (565 nm) | 0.0193 |
| Correction Factor (650 nm) | 0.165 |
| Extinction coefficient (cm -1 M -1) | 250000 |
| Excitation (nm) | 756 |
| Emission (nm) | 779 |
| Quantum yield | 0.3 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Related products
| Overview |  SDSProtocol |
See also: Cyanines
Molecular weight 1291.49 | Correction Factor (260 nm) 0.05 | Correction Factor (280 nm) 0.036 | Correction Factor (482 nm) 0.0005 | Correction Factor (565 nm) 0.0193 | Correction Factor (650 nm) 0.165 | Extinction coefficient (cm -1 M -1) 250000 | Excitation (nm) 756 | Emission (nm) 779 | Quantum yield 0.3 |
Cy7 biotin conjugate demonstrates excellent avidin binding and strong NIR fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. Cy7 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and short wavelength emission. Cy7 Biotin is a water-soluble reagent and its near infrared fluorescence is pH independent from pH 4 to pH 10. A flexible PEG spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Cy7 biotin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 77.43 µL | 387.15 µL | 774.299 µL | 3.871 mL | 7.743 mL |
| 5 mM | 15.486 µL | 77.43 µL | 154.86 µL | 774.299 µL | 1.549 mL |
| 10 mM | 7.743 µL | 38.715 µL | 77.43 µL | 387.15 µL | 774.299 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.05 |
| Correction Factor (280 nm) | 0.036 |
| Correction Factor (482 nm) | 0.0005 |
| Correction Factor (565 nm) | 0.0193 |
| Correction Factor (650 nm) | 0.165 |
| Extinction coefficient (cm -1 M -1) | 250000 |
| Excitation (nm) | 756 |
| Emission (nm) | 779 |
| Quantum yield | 0.3 |
Images
Figure 1. Chemical structure for Cy7 biotin conjugate.
References
View all 6 references: Citation Explorer
Drug-Initiated Synthesis of Heterotelechelic Polymer Prodrug Nanoparticles for in Vivo Imaging and Cancer Cell Targeting.
Authors: Vinciguerra, Daniele and Degrassi, Anna and Mancini, Laura and Mura, Simona and Mougin, Julie and Couvreur, Patrick and Nicolas, Julien
Journal: Biomacromolecules (2019): 2464-2476
Authors: Vinciguerra, Daniele and Degrassi, Anna and Mancini, Laura and Mura, Simona and Mougin, Julie and Couvreur, Patrick and Nicolas, Julien
Journal: Biomacromolecules (2019): 2464-2476
Multistep Consolidated Phototherapy Mediated by a NIR-Activated Photosensitizer.
Authors: Xue, Yudong and Li, Jipeng and Yang, Guoliang and Liu, Zhiyong and Zhou, Huifang and Zhang, Weian
Journal: ACS applied materials & interfaces (2019): 33628-33636
Authors: Xue, Yudong and Li, Jipeng and Yang, Guoliang and Liu, Zhiyong and Zhou, Huifang and Zhang, Weian
Journal: ACS applied materials & interfaces (2019): 33628-33636
Disulfide-based multifunctional conjugates for targeted theranostic drug delivery.
Authors: Lee, Min Hee and Sessler, Jonathan L and Kim, Jong Seung
Journal: Accounts of chemical research (2015): 2935-46
Authors: Lee, Min Hee and Sessler, Jonathan L and Kim, Jong Seung
Journal: Accounts of chemical research (2015): 2935-46
Site-Specific Covalent Labeling of RNA by Enzymatic Transglycosylation.
Authors: Alexander, Seth C and Busby, Kayla N and Cole, Christian M and Zhou, Cun Yu and Devaraj, Neal K
Journal: Journal of the American Chemical Society (2015): 12756-9
Authors: Alexander, Seth C and Busby, Kayla N and Cole, Christian M and Zhou, Cun Yu and Devaraj, Neal K
Journal: Journal of the American Chemical Society (2015): 12756-9
Allo-7: a new fluorescent tandem dye for use in flow cytometry.
Authors: Beavis, A J and Pennline, K J
Journal: Cytometry (1996): 390-5
Authors: Beavis, A J and Pennline, K J
Journal: Cytometry (1996): 390-5
Anomalous fluorescence enhancement of Cy3 and cy3.5 versus anomalous fluorescence loss of Cy5 and Cy7 upon covalent linking to IgG and noncovalent binding to avidin.
Authors: Gruber, H J and Hahn, C D and Kada, G and Riener, C K and Harms, G S and Ahrer, W and Dax, T G and Knaus, H G
Journal: Bioconjugate chemistry : 696-704
Authors: Gruber, H J and Hahn, C D and Kada, G and Riener, C K and Harms, G S and Ahrer, W and Dax, T G and Knaus, H G
Journal: Bioconjugate chemistry : 696-704