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APC-streptavidin conjugate

Flow cytometry analysis of HL-60 cells stained with (Red) or without (Green) 1ug/ml Anti-Human HLA-ABC-Biotin and then followed by APC-streptavidin conjugate (Cat#16902). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the APC channel.
Flow cytometry analysis of HL-60 cells stained with (Red) or without (Green) 1ug/ml Anti-Human HLA-ABC-Biotin and then followed by APC-streptavidin conjugate (Cat#16902). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the APC channel.
Flow cytometry analysis of HL-60 cells stained with (Red) or without (Green) 1ug/ml Anti-Human HLA-ABC-Biotin and then followed by APC-streptavidin conjugate (Cat#16902). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the APC channel.
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Physical properties
Molecular weight~157000
SolventWater
Spectral properties
Correction Factor (280 nm)0.195
Extinction coefficient (cm -1 M -1)730000
Excitation (nm)651
Emission (nm)660
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12352200
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OverviewpdfSDSpdfProtocol


Molecular weight
~157000
Correction Factor (280 nm)
0.195
Extinction coefficient (cm -1 M -1)
730000
Excitation (nm)
651
Emission (nm)
660
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This APC-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with APC covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry and microplate readers. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.

Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.195
Extinction coefficient (cm -1 M -1)730000
Excitation (nm)651
Emission (nm)660

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)Correction Factor (260 nm)
RPE-streptavidin conjugate56557419600000.820.175-
PerCP-streptavidin conjugate477678406000-0.22-
FITC-streptavidin conjugate491516730000.920.35-
Cy3®-streptavidin conjugate55556915000010.1510.0730.07
Cy5®-streptavidin conjugate65167025000010.271, 0.420.030.02
Cy7®-streptavidin conjugate7567792500000.30.0360.05
XFD350-streptavidin conjugate *XFD350 Same Structure to Alexa Fluor™ 350*34344119000-0.190.25
XFD488-streptavidin conjugate *XFD488 Same Structure to Alexa Fluor™ 488*499520730000.9210.110.3
XFD594-streptavidin conjugate *XFD594 Same Structure to Alexa Fluor™ 594*590618920000.6610.560.43

Images


Citations


View all 1 citations: Citation Explorer
Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996

References


View all 47 references: Citation Explorer
A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647
Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50
Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label
Authors: Sun M, Du L, Gao S, Bao Y, Wang S.
Journal: Steroids (2010): 400
Multimodality nuclear and fluorescence tumor imaging in mice using a streptavidin nanoparticle
Authors: Liang M, Liu X, Cheng D, Liu G, Dou S, Wang Y, Rusckowski M, Hnatowich DJ.
Journal: Bioconjug Chem (2010): 1385
Site-dependent excited-state dynamics of a fluorescent probe bound to avidin and streptavidin
Authors: Furstenberg A, Kel O, Gradinaru J, Ward TR, Emery D, Bollot G, Mareda J, Vauthey E.
Journal: Chemphyschem (2009): 1517
Influence of streptavidin on the absorption and fluorescence properties of cyanine dyes
Authors: Luschtinetz F, Dosche C, Kumke MU.
Journal: Bioconjug Chem (2009): 576
Fluorescent nanoscale detection of biotin-streptavidin interaction using near-field scanning optical microscopy
Authors: Park HK, Gokarna A, Hulme JP, Park HG, Chung BH.
Journal: Nanotechnology (2008): 235103
Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives
Authors: Ebner A, Marek M, Kaiser K, Kada G, Hahn CD, Lackner B, Gruber HJ.
Journal: Methods Mol Biol (2008): 73