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APC-iFluor® 750-streptavidin conjugate

HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by APC-iFluor® 750-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel
HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by APC-iFluor® 750-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel
HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by APC-iFluor® 750-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel
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Physical properties
Molecular weight~157000
Spectral properties
Extinction coefficient (cm -1 M -1)700000
Excitation (nm)651
Emission (nm)791
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
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Molecular weight
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This APC-iFluor® 750-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with APC-iFluor® 750 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Cy7® filter set. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.


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Spectral properties

Extinction coefficient (cm -1 M -1)700000
Excitation (nm)651
Emission (nm)791

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
RPE-iFluor® 750-streptavidin conjugate5657781960000



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