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APC-iFluor® 750-streptavidin conjugate

HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by APC-iFluor® 750-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel
HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by APC-iFluor® 750-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel
Ordering information
Price ()
Catalog Number16908
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight157000
SolventWater
Spectral properties
Extinction coefficient (cm -1 M -1)700000
Excitation (nm)651
Emission (nm)791
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
157000
Extinction coefficient (cm -1 M -1)
700000
Excitation (nm)
651
Emission (nm)
791
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This APC-iFluor® 750-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with APC-iFluor® 750 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Cy7® filter set. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of APC-iFluor® 750-streptavidin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM636.943 nL3.185 µL6.369 µL31.847 µL63.694 µL
5 mM127.389 nL636.943 nL1.274 µL6.369 µL12.739 µL
10 mM63.694 nL318.471 nL636.943 nL3.185 µL6.369 µL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)700000
Excitation (nm)651
Emission (nm)791

Citations


View all 1 citations: Citation Explorer
Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996

References


View all 97 references: Citation Explorer
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Journal: European review for medical and pharmacological sciences (2019): 8109-8114
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Authors: Van der Heyden, Hervé and Wallon, Thérèse and Lévesque, C André and Carisse, Odile
Journal: Plant disease (2019): 475-483
Minor groove binder modification of widely used TaqMan hydrolysis probe for detection of dengue virus reduces risk of false-negative real-time PCR results for serotype 4.
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