APC-iFluor® 750-streptavidin conjugate

HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by APC-iFluor® 750-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	~157000
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	791

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

~157000

Extinction coefficient (cm ^-1 M ^-1)

700000

Excitation (nm)

651

Emission (nm)

791

Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This APC-iFluor® 750-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with APC-iFluor® 750 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Cy7® filter set. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.

Spectrum

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Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	791

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
RPE-iFluor® 750-streptavidin conjugate	565	778	1960000

Images

Figure 1. HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by APC-iFluor® 750-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel

Citations

View all 1 citations: Citation Explorer

Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996

References

View all 97 references: Citation Explorer

Novel distamycin analogues that block the cell cycle of African trypanosomes with high selectivity and potency.
Authors: Franco, Jaime and Scarone, Laura and Comini, Marcelo A
Journal: European journal of medicinal chemistry (2020): 112043

Correlations of IL-6 and CRP gene polymorphisms with pulmonary heart disease.
Authors: Wu, Y and Bai, S-R and Wei, S-J and Ge, L-J and Xi, S-J
Journal: European review for medical and pharmacological sciences (2019): 8109-8114

Detection and Quantification of Pythium tracheiphilum in Soil by Multiplex Real-Time qPCR.
Authors: Van der Heyden, Hervé and Wallon, Thérèse and Lévesque, C André and Carisse, Odile
Journal: Plant disease (2019): 475-483

Minor groove binder modification of widely used TaqMan hydrolysis probe for detection of dengue virus reduces risk of false-negative real-time PCR results for serotype 4.
Authors: Gray, Eleanor R and Heaney, Judith and Ferns, R Bridget and Sequeira, Patricia C and Nastouli, Eleni and Garson, Jeremy A
Journal: Journal of virological methods (2019): 17-23

A Novel Broad Allele-Specific TaqMan Real-Time PCR Method To Detect Triazole-Resistant Strains of Aspergillus fumigatus, Even with a Very Low Percentage of Triazole-Resistant Cells Mixed with Triazole-Susceptible Cells.
Authors: Wang, Qian and Kontoyiannis, Dimitrios P and Li, Ruoyu and Chen, Wei and Bu, Dingfang and Liu, Wei
Journal: Journal of clinical microbiology (2019)

Correlation of IL-33 gene polymorphism with chronic obstructive pulmonary disease.
Authors: Sun, B-B and Ma, L-J and Qi, Y and Zhang, G-J
Journal: European review for medical and pharmacological sciences (2019): 6277-6282

Detection and quantification of Aeromonas schubertii in Channa maculata by TaqMan MGB probe fluorescence real-time quantitative PCR.
Authors: Liu, Chun and Guo, Yanming M and Cao, Jizhen Z and Zhang, De-Feng and Chang, Ou-Qin and Li, Kaibin and Wang, Fang and Shi, Cun-Bin and Jiang, Lan and Wang, Qing and Lin, Li
Journal: Journal of fish diseases (2019): 109-117

Development of a TaqMan MGB RT-PCR assay for the detection of type A and subtype H10 avian influenza viruses.
Authors: Yang, Fan and Chen, Bin and Liu, Fumin and Peng, Xiuming and Sun, Tao and Yao, Hangping and Wu, Haibo and Wu, Nanping
Journal: Archives of virology (2018): 2497-2501

Detection of KIT Genotype in Pigs by TaqMan MGB Real-Time Quantitative Polymerase Chain Reaction.
Authors: Li, Xiuxiu and Li, Xiaoning and Luo, Rongrong and Wang, Wenwen and Wang, Tao and Tang, Hui
Journal: DNA and cell biology (2018): 457-464

A rapid minor groove binder PCR method for distinguishing the vaccine strain Brucella abortus 104M.
Authors: Nan, Wenlong and Qin, Lide and Wang, Yong and Zhang, Yueyong and Tan, Pengfei and Chen, Yuqi and Mao, Kairong and Chen, Yiping
Journal: BMC veterinary research (2018): 27