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APC-iFluor® 750-streptavidin conjugate

Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This APC-iFluor® 750-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with APC-iFluor® 750 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Cy7® filter set. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
RPE-iFluor® 750-streptavidin conjugate5657781960000

Citations

View all 1 citations: Citation Explorer
Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996

References

View all 97 references: Citation Explorer
Novel distamycin analogues that block the cell cycle of African trypanosomes with high selectivity and potency.
Authors: Franco, Jaime and Scarone, Laura and Comini, Marcelo A
Journal: European journal of medicinal chemistry (2020): 112043
Correlations of IL-6 and CRP gene polymorphisms with pulmonary heart disease.
Authors: Wu, Y and Bai, S-R and Wei, S-J and Ge, L-J and Xi, S-J
Journal: European review for medical and pharmacological sciences (2019): 8109-8114
Detection and Quantification of Pythium tracheiphilum in Soil by Multiplex Real-Time qPCR.
Authors: Van der Heyden, Hervé and Wallon, Thérèse and Lévesque, C André and Carisse, Odile
Journal: Plant disease (2019): 475-483
Minor groove binder modification of widely used TaqMan hydrolysis probe for detection of dengue virus reduces risk of false-negative real-time PCR results for serotype 4.
Authors: Gray, Eleanor R and Heaney, Judith and Ferns, R Bridget and Sequeira, Patricia C and Nastouli, Eleni and Garson, Jeremy A
Journal: Journal of virological methods (2019): 17-23
A Novel Broad Allele-Specific TaqMan Real-Time PCR Method To Detect Triazole-Resistant Strains of Aspergillus fumigatus, Even with a Very Low Percentage of Triazole-Resistant Cells Mixed with Triazole-Susceptible Cells.
Authors: Wang, Qian and Kontoyiannis, Dimitrios P and Li, Ruoyu and Chen, Wei and Bu, Dingfang and Liu, Wei
Journal: Journal of clinical microbiology (2019)
Page updated on October 22, 2024

Ordering information

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Catalog Number16908
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Additional ordering information

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Physical properties

Molecular weight

~157000

Solvent

Water

Spectral properties

Extinction coefficient (cm -1 M -1)

700000

Excitation (nm)

651

Emission (nm)

791

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure
UNSPSC12352200
HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by APC-iFluor® 750-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel
HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by APC-iFluor® 750-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel
HL-60 cells were incubated with (Red, +) or without (Green, -) mouse Anti-Human HLA-ABC Biotin (W6/32-Biotin) followed by APC-iFluor® 750-streptavidin conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-C7 channel