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CytoFix™ Red Mitochondrial Stain
CytoFix™ Red mitochondrial stain is a dye that selectively stains mitochondria independent of mitochondrial membrane potential gradient. Due to this functionality, CytoFix™ Red mitochondrial stain is well retained in mitochondria even after fixation. The dye permeates intact live cells and gets trapped in live cells. Its key features include high staining efficiency, long retention after fixation with minimal hands on time. CytoFix™ Red mitochondrial stain can be used with GFP expressed cells without overlapping the fluorescence of GFP, making it useful for multiplexing analysis. It can be used for both suspension and adherent cells and readily adapted for a wide variety of fluorescence platforms.
The fluorescence images of HeLa cells stained with CytoFix™ MitoRed in a 96-well black-wall clear-bottom plate. Images were acquired before (Left) and after (Right) fixation with 4% formaldehyde solution for 20 minutes at RT. The cells were imaged using a fluorescence microscope equipped with a Cy3/TRITC filter.
The fluorescence images of HeLa cells stained with CytoFix™ MitoRed in a 96-well black-wall clear-bottom plate. Images were acquired before (Left) and after (Right) fixation with 4% formaldehyde solution for 20 minutes at RT. The cells were imaged using a fluorescence microscope equipped with a Cy3/TRITC filter.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
23200500 Tests
Price
 
Physical properties
Molecular weightN/A
SolventDMSO
Spectral properties
Excitation (nm)564
Emission (nm)651
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
CASN/A
Instrument settings
Fluorescence microscope
ExcitationCy3/TRITC filter set
EmissionCy3/TRITC filter set
Recommended plateBlack wall/clear bottom
Instrument specification(s)Cy3/TRITC filter set
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 24, 2025