DAPI | AAT Bioquest

DAPI Dilactate

DAPI is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. Since DAPI passes through an intact cell membrane, it can be used to stain live cells besides fixed cells. For fluorescence microscopy, DAPI is excited with ultraviolet light. When bound to double-stranded DNA its absorption maximum is at 358 nm and its emission maximum is at 461 nm. One drawback of DAPI is that its emission is fairly broad. DAPI also binds to RNA although it is not as strongly fluorescent as it binds to DNA. Its emission shifts to around 500 nm when bound to RNA. DAPI's blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Besides labeling cell nuclei, DAPI is also used for the detection of mycoplasma or virus DNA in cell cultures. DAPI dilactate has improved water solubility compared to DAPI HCl salt.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17509	25 mg	Price

References

View all 48 references: Citation Explorer

Differential pulse voltammetric studies of ethidium bromide binding to DNA

Authors:

Minasyan SH, Tavadyan LA, Antonyan AP, Davtyan HG, Parsadanyan MA, Vardevanyan PO.

Journal:

Bioelectrochemistry (2006): 48

Possible errors in the interpretation of ethidium bromide and PicoGreen DNA staining results from ethidium monoazide-treated DNA

Authors:

Hein I, Flekna G, Wagner M, Nocker A, Camper AK.

Journal:

Appl Environ Microbiol (2006): 6860

Ethidium bromide inhibits rat brain acetylcholinesterase activity in vitro

Authors:

Mazzanti CM, Spanevello RM, Obregon A, Pereira LB, Streher CA, Ahmed M, Mazzanti A, Graca DL, Morsch VM, Schetinger MR.

Journal:

Chem Biol Interact (2006): 121

Delayed Schwann cell and oligodendrocyte remyelination after ethidium bromide injection in the brainstem of Wistar rats submitted to streptozotocin diabetogenic treatment

Authors:

Bondan EF, Lallo MA, Trigueiro AH, Ribeiro CP, Sinhorini IL, Graca DL.

Journal:

Braz J Med Biol Res (2006): 637

A low dose of ethidium bromide leads to an increase of total mitochondrial DNA while higher concentrations induce the mtDNA 4997 deletion in a human neuronal cell line

Authors:

von Wurmb-Schwark N, Cavelier L, Cortopassi GA.

Journal:

Mutat Res (2006): 57

Physical properties

Molecular weight	457.48
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	27000
Excitation (nm)	359
Emission (nm)	457

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134
CAS	28718-91-4

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Page updated on September 19, 2024