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DAPI Dilactate

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Physical properties
Molecular weight457.48
Spectral properties
Extinction coefficient (cm -1 M -1)27000
Excitation (nm)359
Emission (nm)457
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
DAPI is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. Since DAPI passes through an intact cell membrane, it can be used to stain live cells besides fixed cells. For fluorescence microscopy, DAPI is excited with ultraviolet light. When bound to double-stranded DNA its absorption maximum is at 358 nm and its emission maximum is at 461 nm. One drawback of DAPI is that its emission is fairly broad. DAPI also binds to RNA although it is not as strongly fluorescent as it binds to DNA. Its emission shifts to around 500 nm when bound to RNA. DAPI's blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Besides labeling cell nuclei, DAPI is also used for the detection of mycoplasma or virus DNA in cell cultures. DAPI dilactate has improved water solubility compared to DAPI HCl salt.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of DAPI Dilactate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM218.589 µL1.093 mL2.186 mL10.929 mL21.859 mL
5 mM43.718 µL218.589 µL437.178 µL2.186 mL4.372 mL
10 mM21.859 µL109.294 µL218.589 µL1.093 mL2.186 mL

Molarity calculator

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Spectral properties

Extinction coefficient (cm -1 M -1)27000
Excitation (nm)359
Emission (nm)457



View all 48 references: Citation Explorer
Differential pulse voltammetric studies of ethidium bromide binding to DNA
Authors: Minasyan SH, Tavadyan LA, Antonyan AP, Davtyan HG, Parsadanyan MA, Vardevanyan PO.
Journal: Bioelectrochemistry (2006): 48
Immunohistochemical staining of the macrophagic and astrocytic response in the brainstem of Wistar rats submitted to the ethidium bromide gliotoxic model and treated with cyclophosphamide
Authors: Sanchez M, Bondan EF, Lallo MA, Sinhorini IL, Dagli ML, Maiorka PC, Pereira LA, Graca DL.
Journal: Arq Neuropsiquiatr (2006): 787
Use of ethidium bromide monoazide for quantification of viable and dead mixed bacterial flora from fish fillets by polymerase chain reaction
Authors: Lee JL, Levin RE.
Journal: J Microbiol Methods. (2006)
Analysis of cycloheximide-induced apoptosis in human leukocytes: Fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide
Authors: Baskic D, Popovic S, Ristic P, Arsenijevic NN.
Journal: Cell Biol Int (2006): 924
Role of the ABC transporter TruMDR2 in terbinafine, 4-nitroquinoline N-oxide and ethidium bromide susceptibility in Trichophyton rubrum
Authors: Fachin AL, Ferreira-Nozawa MS, Maccheroni W, Jr., Martinez-Rossi NM.
Journal: J Med Microbiol (2006): 1093
Atomic force microscopic examination of chromosomes treated with trypsin or ethidium bromide
Authors: Wu Y, Cai J, Cheng L, Yun K, Wang C, Chen Y.
Journal: Chem Pharm Bull (Tokyo) (2006): 501
Acetylcholinesterase activity in rats experimentally demyelinated with ethidium bromide and treated with interferon beta
Authors: Mazzanti CM, Spanevello RM, Pereira LB, Goncalves JF, Kaizer R, Correa M, Ahmed M, Mazzanti A, Festugatto R, Graca DL, Morsch VM, Schetinger MR.
Journal: Neurochem Res (2006): 1027
Extended ethidium bromide analogue as a triple helix intercalator: synthesis, photophysical properties and nucleic acids binding
Authors: Tam VK, Liu Q, Tor Y.
Journal: Chem Commun (Camb) (2006): 2684
The Complex of Ethidium Bromide with Genomic DNA: Structure Analysis by Polarized Raman Spectroscopy
Authors: Tsuboi M, Benevides JM, Thomas Jr GJ.
Journal: Biophys J. (2006)
Apyrase and 5'-nucleotidase activities in synaptosomes from the cerebral cortex of rats experimentally demyelinated with ethidium bromide and treated with interferon-beta
Authors: Spanevello RM, Mazzanti CM, Kaizer R, Zanin R, Cargnelutti D, Hannel L, Correa M, Mazzanti A, Festugatto R, Graca D, Schetinger MR, Morsch VM.
Journal: Neurochem Res (2006): 455