DAPI Dilactate

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Physical properties

Molecular weight	457.48
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	27000
Excitation (nm)	359
Emission (nm)	457

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Overview SDS Protocol

CAS

28718-91-4

Molecular weight

457.48

Extinction coefficient (cm ^-1 M ^-1)

27000

Excitation (nm)

359

Emission (nm)

457

DAPI is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. Since DAPI passes through an intact cell membrane, it can be used to stain live cells besides fixed cells. For fluorescence microscopy, DAPI is excited with ultraviolet light. When bound to double-stranded DNA its absorption maximum is at 358 nm and its emission maximum is at 461 nm. One drawback of DAPI is that its emission is fairly broad. DAPI also binds to RNA although it is not as strongly fluorescent as it binds to DNA. Its emission shifts to around 500 nm when bound to RNA. DAPI's blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Besides labeling cell nuclei, DAPI is also used for the detection of mycoplasma or virus DNA in cell cultures. DAPI dilactate has improved water solubility compared to DAPI HCl salt.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of DAPI Dilactate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	218.589 µL	1.093 mL	2.186 mL	10.929 mL	21.859 mL
5 mM	43.718 µL	218.589 µL	437.178 µL	2.186 mL	4.372 mL
10 mM	21.859 µL	109.294 µL	218.589 µL	1.093 mL	2.186 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	27000
Excitation (nm)	359
Emission (nm)	457

Images

Figure 1. Chemical structure for DAPI Dilactate

References

View all 48 references: Citation Explorer

Differential pulse voltammetric studies of ethidium bromide binding to DNA
Authors: Minasyan SH, Tavadyan LA, Antonyan AP, Davtyan HG, Parsadanyan MA, Vardevanyan PO.
Journal: Bioelectrochemistry (2006): 48

Immunohistochemical staining of the macrophagic and astrocytic response in the brainstem of Wistar rats submitted to the ethidium bromide gliotoxic model and treated with cyclophosphamide
Authors: Sanchez M, Bondan EF, Lallo MA, Sinhorini IL, Dagli ML, Maiorka PC, Pereira LA, Graca DL.
Journal: Arq Neuropsiquiatr (2006): 787

Use of ethidium bromide monoazide for quantification of viable and dead mixed bacterial flora from fish fillets by polymerase chain reaction
Authors: Lee JL, Levin RE.
Journal: J Microbiol Methods. (2006)

Analysis of cycloheximide-induced apoptosis in human leukocytes: Fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide
Authors: Baskic D, Popovic S, Ristic P, Arsenijevic NN.
Journal: Cell Biol Int (2006): 924

Role of the ABC transporter TruMDR2 in terbinafine, 4-nitroquinoline N-oxide and ethidium bromide susceptibility in Trichophyton rubrum
Authors: Fachin AL, Ferreira-Nozawa MS, Maccheroni W, Jr., Martinez-Rossi NM.
Journal: J Med Microbiol (2006): 1093

Atomic force microscopic examination of chromosomes treated with trypsin or ethidium bromide
Authors: Wu Y, Cai J, Cheng L, Yun K, Wang C, Chen Y.
Journal: Chem Pharm Bull (Tokyo) (2006): 501

Acetylcholinesterase activity in rats experimentally demyelinated with ethidium bromide and treated with interferon beta
Authors: Mazzanti CM, Spanevello RM, Pereira LB, Goncalves JF, Kaizer R, Correa M, Ahmed M, Mazzanti A, Festugatto R, Graca DL, Morsch VM, Schetinger MR.
Journal: Neurochem Res (2006): 1027

Extended ethidium bromide analogue as a triple helix intercalator: synthesis, photophysical properties and nucleic acids binding
Authors: Tam VK, Liu Q, Tor Y.
Journal: Chem Commun (Camb) (2006): 2684

The Complex of Ethidium Bromide with Genomic DNA: Structure Analysis by Polarized Raman Spectroscopy
Authors: Tsuboi M, Benevides JM, Thomas Jr GJ.
Journal: Biophys J. (2006)

Apyrase and 5'-nucleotidase activities in synaptosomes from the cerebral cortex of rats experimentally demyelinated with ethidium bromide and treated with interferon-beta
Authors: Spanevello RM, Mazzanti CM, Kaizer R, Zanin R, Cargnelutti D, Hannel L, Correa M, Mazzanti A, Festugatto R, Graca D, Schetinger MR, Morsch VM.
Journal: Neurochem Res (2006): 455