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# DAPI

4,6-Diamidino-2-phenylindole, dihydrochloride; 10 mM solution in water
DAPI is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. Since DAPI passes through an intact cell membrane, it can be used to stain live cells besides fixed cells. For fluorescence microscopy, DAPI is excited with ultraviolet light. When bound to double-stranded DNA its absorption maximum is at 358 nm and its emission maximum is at 461 nm. One drawback of DAPI is that its emission is fairly broad. DAPI also binds to RNA although it is not as strongly fluorescent as it binds to DNA. Its emission shifts to around 500 nm when bound to RNA. DAPI's blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Besides labeling cell nuclei, DAPI is also used for the detection of mycoplasma or virus DNA in cell cultures.

## Calculators

### Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *10 mM solution in water* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

 0.1 mg 0.5 mg 1 mg 5 mg 10 mg 1 mM 285.51 µL 1.428 mL 2.855 mL 14.276 mL 28.551 mL 5 mM 57.102 µL 285.51 µL 571.021 µL 2.855 mL 5.71 mL 10 mM 28.551 µL 142.755 µL 285.51 µL 1.428 mL 2.855 mL

### Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

 Mass (Calculate) Molecular weight Volume (Calculate) Concentration (Calculate) Moles fgpgngµgmgg / g/mol = fLpLnLµLmLL x fMpMnMµMmMM = fmolpmolnmolµmolmmolmol

## Citations

View all 51 citations: Citation Explorer
An NIR-responsive hydrogel loaded with polydeoxyribonucleotide nano-vectors for enhanced chronic wound healing
Authors: Sun, Yanzhen and Li, Yao and Ding, Xiaokang and Xu, Pan and Jing, Xiaodong and Cong, Hailin and Hu, Hao and Yu, Bing and Xu, Fu-Jian
Journal: Biomaterials (2024): 122789
Deciphering the Role of Ferroptosis in Rheumatoid Arthritis: Synovial Transcriptome Analysis and Immune Infiltration Correlation
Authors: Wang, Hongli and Zhang, Miaomiao and Hu, Yiping and He, Juan and Zhong, Yuchao and Dai, Yong and Wang, Qingwen
Journal: Heliyon (2024)
The mesenchymal stromal cell (MSC) secretome promotes tissue regeneration and increases macrophage infiltration in acute and MRSA-infected skin wounds in vivo
Authors: Rajesh, Aarthi and Ju, Esther Da Eun and Oxford, Kelly A and Harman, Rebecca M and Van de Walle, Gerlinde R
Journal: Cytotherapy (2024)
Adipose Tissue and Translation Machinery in Metabolic Regulation
Authors: De Siqueira, Mirian Krystel
Journal: (2024)
Mechanosensitive membrane domains regulate calcium entry in arterial endothelial cells to protect against inflammation
Authors: Hong, Soon-Gook and Ashby, Julianne W and Kennelly, John P and Wu, Meigan and Steel, Michelle and Chattopadhyay, Eesha and Foreman, Rob and Tontonoz, Peter and Tarling, Elizabeth J and Turowski, Patric and others,
Journal: The Journal of Clinical Investigation (2024)

## References

View all 134 references: Citation Explorer
Reliable DNA ploidy determination in dehydrated tissues of vascular plants by DAPI flow cytometry--new prospects for plant research
Authors: Suda J, Travnicek P.
Journal: Cytometry A (2006): 273
Identification of all pachytene bivalents in the common shrew using DAPI-staining of synaptonemal complex spreads
Authors: Belonogova NM, Karamysheva TV, Biltueva LS, Perepelov EA, Minina JM, Polyakov AV, Zhdanova NS, Rubtsov NB, Searle JB, Borodin PM.
Journal: Chromosome Res (2006): 673
Physical localization of ribosomal genes and chromosome DAPI banding by in situ hybridization in Medicago sativa L
Authors: Chen JM, Hong YH, Wang YP, Bowley S, Wan JM.
Journal: Yi Chuan (2006): 184
DNA staining with the fluorochromes EtBr, DAPI and YOYO-1 in the comet assay with tobacco plants after treatment with ethyl methanesulphonate, hyperthermia and DNase-I
Authors: Gichner T, Mukherjee A, Veleminsky J.
Journal: Mutat Res (2006): 17
Investigation of chromosomes in varieties and translocation lines of pea Pisum sativum L. by FISH, Ag-NOR, and differential DAPI staining
Authors: Samatadze TE, Muravenko OM, Bol'sheva NL, Amosova AB, Gostimsckii SA, Zelenin AV.
Journal: Genetika (2005): 1665
Page updated on September 5, 2024

## Ordering information

 Price Form 10 mM aqueous solution10 mM aqueous solutionPowder Unit size Catalog Number 17507175101751117513 Quantity

 Telephone 1-800-990-8053 Fax 1-800-609-2943 Email sales@aatbio.com International See distributors Bulk request Inquire Custom size Inquire Technical Support Contact us Purchase order Send to sales@aatbio.com Shipping Standard overnight for United States, inquire for international

350.25

Water

27000

359

457

## Storage, safety and handling

H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68

### Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC41116134

28718-90-3

## Alternative formats

 DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*