Name: Diazepam HRP Conjugate
Brand: AAT Bioquest
SKU: 50667
Price: 989 USD
Availability: InStock

Diazepam HRP Conjugate

Diazepam HRP conjugate is an enzyme-linked benzodiazepine tracer combining diazepam with horseradish peroxidase, designed for competitive ELISA assays to detect diazepam and related benzodiazepines in biological samples.

Well-Characterized Tracer: Diazepam is covalently linked to horseradish peroxidase (HRP) for use in competitive ELISAs and related immunoassays
High Sensitivity and Specificity: Enables detection and quantification of diazepam and structurally related benzodiazepines in complex biological matrices such as plasma, serum, and urine
Versatile Substrate Compatibility: HRP catalyzes chromogenic (e.g., TMB, ABTS), fluorogenic, and chemiluminescent (e.g., luminol-based) reactions for enhanced signal amplification
Broad Application Range: Suitable for clinical toxicology, forensic analysis, therapeutic drug monitoring, and pharmacological research

The chemical structure of Diazepam HRP Conjugate.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
50667	1 mg	Price

Overview

Diazepam HRP is a well-characterized conjugate consisting of diazepam covalently linked to horseradish peroxidase (HRP), designed for use as a tracer in competitive enzyme-linked immunosorbent assays (ELISAs) and related immunodetection platforms. The conjugate enables sensitive and specific quantification of diazepam and structurally related benzodiazepines in complex biological matrices, including plasma, serum, and urine. The HRP enzyme catalyzes the oxidation of chromogenic (e.g., TMB, ABTS), fluorogenic, or chemiluminescent (e.g., luminol-based) substrates, facilitating signal amplification and improving assay detection limits. In competitive assay formats, Diazepam HRP competes with free diazepam for binding to immobilized anti-diazepam antibodies, with signal intensity inversely proportional to the concentration of free analyte. Diazepam HRP is suitable for a wide range of applications in clinical toxicology, forensic analysis, therapeutic drug monitoring, and pharmacological research.

References

View all 50 references: Citation Explorer

Intranasal Coadministration of a Diazepam Prodrug with a Converting Enzyme Results in Rapid Absorption of Diazepam in Rats.

Authors:

Rautiola, Davin and Maglalang, Patricia D and Cheryala, Narsihmulu and Nelson, Kathryn M and Georg, Gunda I and Fine, Jared M and Svitak, Aleta L and Faltesek, Katherine A and Hanson, Leah R and Mishra, Usha and Coles, Lisa D and Cloyd, James C and Siegel, Ronald A

Journal:

The Journal of pharmacology and experimental therapeutics (2019): 796-805

Determining the effects of adulterants on drug detection via enzyme-linked immunosorbent assay and adulterant tests strips.

Authors:

Olivieri, Bianca and Marić, Mark and Bridge, Candice

Journal:

Drug testing and analysis (2018): 1383-1393

Inhibition of the endocannabinoid-regulating enzyme monoacylglycerol lipase elicits a CB1 receptor-mediated discriminative stimulus in mice.

Authors:

Owens, Robert A and Mustafa, Mohammed A and Ignatowska-Jankowska, Bogna M and Damaj, M Imad and Beardsley, Patrick M and Wiley, Jenny L and Niphakis, Micah J and Cravatt, Benjamin F and Lichtman, Aron H

Journal:

Neuropharmacology (2017): 80-86

Anti-depressant and anxiolytic potential of Acacia hydaspica R. Parker aerial parts extract: Modulation of brain antioxidant enzyme status.

Authors:

Afsar, Tayyaba and Razak, Suhail and Khan, Muhammad Rashid and Almajwal, Ali

Journal:

BMC complementary and alternative medicine (2017): 228

Clinical Potential of an Enzyme-based Novel Therapy for Cocaine Overdose.

Authors:

Zhang, Ting and Zheng, Xirong and Zhou, Ziyuan and Chen, Xiabin and Jin, Zhenyu and Deng, Jing and Zhan, Chang-Guo and Zheng, Fang

Journal:

Scientific reports (2017): 15303

Physical properties

Solvent

DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on May 1, 2026