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Diazepam HRP Conjugate

Product key features

  • Well-characterized tracer: Diazepam is covalently linked to horseradish peroxidase (HRP) for use in competitive ELISAs and related immunoassays.
  • High sensitivity and specificity: Enables detection and quantification of diazepam and structurally related benzodiazepines in complex biological matrices such as plasma, serum, and urine.
  • Versatile substrate compatibility: HRP catalyzes chromogenic (e.g., TMB, ABTS), fluorogenic, and chemiluminescent (e.g., luminol-based) reactions for enhanced signal amplification.
  • Broad application range: Suitable for clinical toxicology, forensic analysis, therapeutic drug monitoring, and pharmacological research.

Product description

Diazepam HRP is a well-characterized conjugate consisting of diazepam covalently linked to horseradish peroxidase (HRP), designed for use as a tracer in competitive enzyme-linked immunosorbent assays (ELISAs) and related immunodetection platforms. The conjugate enables sensitive and specific quantification of diazepam and structurally related benzodiazepines in complex biological matrices, including plasma, serum, and urine. The HRP enzyme catalyzes the oxidation of chromogenic (e.g., TMB, ABTS), fluorogenic, or chemiluminescent (e.g., luminol-based) substrates, facilitating signal amplification and improving assay detection limits. In competitive assay formats, Diazepam HRP competes with free diazepam for binding to immobilized anti-diazepam antibodies, with signal intensity inversely proportional to the concentration of free analyte. Diazepam HRP is suitable for a wide range of applications in clinical toxicology, forensic analysis, therapeutic drug monitoring, and pharmacological research.

References

View all 50 references: Citation Explorer
Intranasal Coadministration of a Diazepam Prodrug with a Converting Enzyme Results in Rapid Absorption of Diazepam in Rats.
Authors: Rautiola, Davin and Maglalang, Patricia D and Cheryala, Narsihmulu and Nelson, Kathryn M and Georg, Gunda I and Fine, Jared M and Svitak, Aleta L and Faltesek, Katherine A and Hanson, Leah R and Mishra, Usha and Coles, Lisa D and Cloyd, James C and Siegel, Ronald A
Journal: The Journal of pharmacology and experimental therapeutics (2019): 796-805
Determining the effects of adulterants on drug detection via enzyme-linked immunosorbent assay and adulterant tests strips.
Authors: Olivieri, Bianca and Marić, Mark and Bridge, Candice
Journal: Drug testing and analysis (2018): 1383-1393
Inhibition of the endocannabinoid-regulating enzyme monoacylglycerol lipase elicits a CB1 receptor-mediated discriminative stimulus in mice.
Authors: Owens, Robert A and Mustafa, Mohammed A and Ignatowska-Jankowska, Bogna M and Damaj, M Imad and Beardsley, Patrick M and Wiley, Jenny L and Niphakis, Micah J and Cravatt, Benjamin F and Lichtman, Aron H
Journal: Neuropharmacology (2017): 80-86
Anti-depressant and anxiolytic potential of Acacia hydaspica R. Parker aerial parts extract: Modulation of brain antioxidant enzyme status.
Authors: Afsar, Tayyaba and Razak, Suhail and Khan, Muhammad Rashid and Almajwal, Ali
Journal: BMC complementary and alternative medicine (2017): 228
Clinical Potential of an Enzyme-based Novel Therapy for Cocaine Overdose.
Authors: Zhang, Ting and Zheng, Xirong and Zhou, Ziyuan and Chen, Xiabin and Jin, Zhenyu and Deng, Jing and Zhan, Chang-Guo and Zheng, Fang
Journal: Scientific reports (2017): 15303
Page updated on June 30, 2025

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Catalog Number50667
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Physical properties

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure