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DiBAC4(5) [Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol]

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Physical properties
Molecular weight542.67
Spectral properties
Excitation (nm)591
Emission (nm)615
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
DiBAC4(5) is a sensitive membrane potential probe with longer excitation and emission wavelength. It is a slow-response probe for measuring cellular membrane potential. In general, slow-response probes exhibit potential-dependent changes in their transmembrane distribution that are accompanied by a fluorescence change. The magnitude of their optical responses is much larger than that of fast-response probes (typically a 1% fluorescence change per mV). Slow-response probes, which include cationic carbocyanines, rhodamines and anionic oxonols, are suitable for detecting changes in average membrane potentials of nonexcitable cells caused by respiratory activity, ion-channel permeability, drug binding and other factors.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiBAC4(5) [Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM184.274 µL921.37 µL1.843 mL9.214 mL18.427 mL
5 mM36.855 µL184.274 µL368.548 µL1.843 mL3.685 mL
10 mM18.427 µL92.137 µL184.274 µL921.37 µL1.843 mL

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Spectral properties

Excitation (nm)591
Emission (nm)615



View all 5 citations: Citation Explorer
A method for quantifying sporulation efficiency and isolating meiotic progeny in non-GMO strains of Saccharomyces cerevisiae
Authors: Sirr, Amy and Timour, Martin S and Cromie, Gareth A and Tang, Michelle and Dudley, Aim{\'e}e M
Journal: Yeast (2022): 354--362
A Membrane Potential-and Calpain-Dependent Reversal of Caspase-1 Inhibition Regulates Canonical NLRP3 Inflammasome
Authors: Zhang, Yifei and Rong, Hua and Zhang, Fang-Xiong and Wu, Kun and Mu, Libing and Meng, Junchen and Xiao, Bailong and Zamponi, Gerald W and Shi, Yan
Journal: Cell reports (2018): 2356--2369
Effects of 1, 4-naphthoquinone Aged Carbon Black Particles on the Cell Membrane of Human Bronchial Epithelium
Authors: Zhang, Yongming and Wang, Li and Feng, Huimin and Hu, Guiping and Wang, Lele and Liu, Jiaxing and Gao, Xin and Shang, Jing and Zhu, Tong and Tang, Shichuan and others, undefined
Journal: Environmental Toxicology and Pharmacology (2017)
Humic analog AQDS can act as a selective inhibitor to enable anoxygenic photosynthetic bacteria to outcompete sulfate-reducing bacteria under microaerobic conditions
Authors: Wang, Xingzu and Cheng, Xiang and Ren, Yiwei and Xu, Guihua and Tang, Jing
Journal: Journal of Chemical Technology and Biotechnology (2015)
Mitochondrial ROS-K+ channel signaling pathway regulated secretion of human pulmonary artery endothelial cells
Authors: Ouyang, Jin-Sheng and Li, Yu-Ping and Li, Cheng-Ye and Cai, Chang and Chen, Cheng-Shui and Chen, Shao-Xian and Chen, Yan-Fan and Yang, Li and Xie, Yu-Peng
Journal: Free radical research (2012): 1437--1445


View all 36 references: Citation Explorer
Role of cGMP in sildenafil-induced activation of endothelial Ca2+-activated K+ channels
Authors: Luedders DW, Muenz BM, Li F, Rueckleben S, Tillmanns H, Waldecker B, Wiecha J, Erdogan A, Schaefer CA, Kuhlmann CR.
Journal: J Cardiovasc Pharmacol (2006): 365
Rapid assessment of the physiological status of Streptococcus macedonicus by flow cytometry and fluorescence probes
Authors: Papadimitriou K, Pratsinis H, Nebe-von-Caron G, Kletsas D, Tsakalidou E.
Journal: Int J Food Microbiol (2006): 197
N-terminal parathyroid hormone-related peptide hyperpolarizes endothelial cells and causes a reduction of the coronary resistance of the rat heart via endothelial hyperpolarization
Authors: Abdallah Y, Ross G, Dolf A, Heinemann MP, Schluter KD.
Journal: Peptides (2006): 2927
The Zn2+-transporting pathways in pancreatic beta-cells: a role for the L-type voltage-gated Ca2+ channel
Authors: Gyulkh, undefined and anyan AV, Lee SC, Bikopoulos G, Dai F, Wheeler MB.
Journal: J Biol Chem (2006): 9361
Activation of Ca(2+)-activated potassium channels is involved in lysophosphatidylcholine-induced monocyte adhesion to endothelial cells
Authors: Erdogan A, Schaefer MB, Kuhlmann CR, Most A, Hartmann M, Mayer K, Renner FC, Schaefer C, Abdallah Y, Hoelschermann H, Schaefer CA.
Journal: Atherosclerosis. (2006)
K+ currents regulate the resting membrane potential, proliferation, and contractile responses in ventricular fibroblasts and myofibroblasts
Authors: Chilton L, Ohya S, Freed D, George E, Drobic V, Shibukawa Y, Maccannell KA, Imaizumi Y, Clark RB, Dixon IM, Giles WR.
Journal: Am J Physiol Heart Circ Physiol (2005): H2931
A new signaling mechanism of hepatocyte growth factor-induced endothelial proliferation
Authors: Kuhlmann CR, Schaefer CA, Fehsecke A, Most AK, Tillmanns H, Erdogan A.
Journal: J Thromb Haemost (2005): 2089
Margatoxin inhibits VEGF-induced hyperpolarization, proliferation and nitric oxide production of human endothelial cells
Authors: Erdogan A, Schaefer CA, Schaefer M, Luedders DW, Stockhausen F, Abdallah Y, Schaefer C, Most AK, Tillmanns H, Piper HM, Kuhlmann CR.
Journal: J Vasc Res (2005): 368
Quercetin-induced induction of the NO/cGMP pathway depends on Ca2+-activated K+ channel-induced hyperpolarization-mediated Ca2+-entry into cultured human endothelial cells
Authors: Kuhlmann CR, Schaefer CA, Kosok C, Abdallah Y, Walther S, Ludders DW, Neumann T, Tillmanns H, Schafer C, Piper HM, Erdogan A.
Journal: Planta Med (2005): 520
Flow cytometry analysis of germinating Bacillus spores, using membrane potential dye
Authors: Laflamme C, Ho J, Veillette M, de Latremoille MC, Verreault D, Meriaux A, Duchaine C.
Journal: Arch Microbiol (2005): 107