AAT Bioquest

DiBAC4(3) [Bis-(1,3-dibutylbarbituric acid)trimethine oxonol]

Product Image
Product Image
Gallery Image 1
The effect of clioquinol in cell membrane. a) Using propidium iodide influx assay to evaluate the influence on plasma membrane integrity; b) Using DiBAC4(3) to evaluate the membrane potential. CQ increased DiBAC4(3) fluorescence intensity by 12.8, 23.8, 113.7, 124.2, 147.8 and 167.2% at different concentrations (1, 2, 4, 8, 16 and 32 μg/ml) compared with untreated cells, respectively. *, p < 0.05; **, p < 0.001; ***, p < 0.0001; DiBAC4(3), bis-(1,3-dibutylbarbituric acid) trimethine oxonol; CQ, clioquinol; AMB, amphotericin B. Source: <b>The effects of clioquinol in morphogenesis, cell membrane and ion homeostasis in <em>Candida albicans</em></b> by Zimeng You, Chaoliang Zhang & Yuping Ran. <em>BMC Microbiology</em>, June 2020.
Ordering information
Catalog Number
Unit Size
Add to cart
Additional ordering information
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight516.64
Spectral properties
Excitation (nm)493
Emission (nm)517
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
DiBAC4(3) is a sensitive slow-response probe for measuring cellular membrane potential. In general, slow-response probes exhibit potential-dependent changes in their transmembrane distribution that are accompanied by a fluorescence change. The magnitude of their optical responses is much larger than that of fast-response probes (typically a 1% fluorescence change per mV). Slow-response probes, which include cationic carbocyanines, rhodamines and anionic oxonols, are suitable for detecting changes in average membrane potentials of nonexcitable cells caused by respiratory activity, ion-channel permeability, drug binding and other factors.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiBAC4(3) [Bis-(1,3-dibutylbarbituric acid)trimethine oxonol] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM193.558 µL967.792 µL1.936 mL9.678 mL19.356 mL
5 mM38.712 µL193.558 µL387.117 µL1.936 mL3.871 mL
10 mM19.356 µL96.779 µL193.558 µL967.792 µL1.936 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles


Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)493
Emission (nm)517



View all 17 citations: Citation Explorer
Co-exposure to pentachlorophenol (PCP) and cadmium (Cd) triggers apoptosis-like cell death in Eschericia coli
Authors: Chen, Zhilan and Jiang, Yi and Lai, Xuebin and Zhu, Chenhong and Zhang, Dapeng and Wang, Hailin
Journal: Environmental Pollution (2024): 123640
Membrane Domain Anti-Registration Induces an Intrinsic Transmembrane Potential
Authors: Lin, Xiaoqian and Lin, Kaidong and He, Shiqi and Zhou, Yue and Li, Xiu and Lin, Xubo
Journal: Langmuir (2023)
Regulation of localized corrosion of 316L stainless steel on osteogenic differentiation of bone morrow derived mesenchymal stem cells
Authors: Li, Meng and Wu, Jing and Geng, Wenbo and Yang, Yulu and Li, Xuan and Xu, Kun and Li, Ke and Li, Yan and Duan, Qiaojian and Gao, Pengfei and others,
Journal: Biomaterials (2023): 122262
Effects of extremely low frequency electromagnetic fields on the tumor cell inhibition and the possible mechanism
Authors: Sun, Jie and Tong, Yingying and Jia, Yu and Jia, Xu and Wang, Hua and Chen, Yang and Wu, Jiamin and Jin, Weiyang and Ma, Zheng and Cao, Kai and others,
Journal: Scientific Reports (2023): 6989
NS1619 Alleviate Brain-Derived Extracellular Vesicle-Induced Brain Injury by Regulating BKca Channel and Nrf2/HO-1/NF-{\sc{k}}B Pathway
Authors: Gao, Yalong and Zhang, Hejun and Li, Xiaotian and Li, Lei and Li, Fanjian and Li, Tuo and Peng, Ruilong and Wang, Cong and Wang, Jiwei and Liu, Xiao and others,
Journal: Oxidative Medicine and Cellular Longevity (2022)
Pitting behavior of 316L stainless steel in direct culture with mesenchymal stem cells
Authors: Wu, Jing and Li, Meng and Zhou, Yang and Xu, Kun and Li, Xuan and Lin, Chuanchuan and Zhang, Jixi and Cai, Kaiyong
Journal: Corrosion Science (2022): 110380
Research Article NS1619 Alleviate Brain-Derived Extracellular Vesicle-Induced Brain Injury by Regulating BKca Channel and Nrf2/HO-1/NF-{\sc{k}}B Pathway
Authors: Gao, Yalong and Zhang, Hejun and Li, Xiaotian and Li, Lei and Li, Fanjian and Li, Tuo and Peng, Ruilong and Wang, Cong and Wang, Jiwei and Liu, Xiao and others,
Journal: (2022)
The effects of clioquinol in morphogenesis, cell membrane and ion homeostasis in Candida albicans
Authors: You, Zimeng and Zhang, Chaoliang and Ran, Yuping
Journal: BMC microbiology (2020): 1--11
Synergistic Effects of 4-Aminopyridine and Paclitaxel on MCF 7 Cell Line
Authors: Aydo{\u{g}}mu{\c{s}}, Esra M{\"u}nire C{\"u}ce and Garip, Gunseli Ayse Inhan
Journal: Clinical and Experimental Health Sciences (2019): 321--327
Enhancement of anaerobic degradation of petroleum hydrocarbons by electron intermediate: Performance and mechanism
Authors: Liu, Xiang and Li, Zhengwen and Zhang, Chen and Tan, Xuejun and Yang, Xue and Wan, Chunli and Lee, Duu-Jong
Journal: Bioresource Technology (2019): 122305


View all 36 references: Citation Explorer
Activation of Ca(2+)-activated potassium channels is involved in lysophosphatidylcholine-induced monocyte adhesion to endothelial cells
Authors: Erdogan A, Schaefer MB, Kuhlmann CR, Most A, Hartmann M, Mayer K, Renner FC, Schaefer C, Abdallah Y, Hoelschermann H, Schaefer CA.
Journal: Atherosclerosis. (2006)
The Zn2+-transporting pathways in pancreatic beta-cells: a role for the L-type voltage-gated Ca2+ channel
Authors: Gyulkh, undefined and anyan AV, Lee SC, Bikopoulos G, Dai F, Wheeler MB.
Journal: J Biol Chem (2006): 9361
N-terminal parathyroid hormone-related peptide hyperpolarizes endothelial cells and causes a reduction of the coronary resistance of the rat heart via endothelial hyperpolarization
Authors: Abdallah Y, Ross G, Dolf A, Heinemann MP, Schluter KD.
Journal: Peptides (2006): 2927
Rapid assessment of the physiological status of Streptococcus macedonicus by flow cytometry and fluorescence probes
Authors: Papadimitriou K, Pratsinis H, Nebe-von-Caron G, Kletsas D, Tsakalidou E.
Journal: Int J Food Microbiol (2006): 197
Role of cGMP in sildenafil-induced activation of endothelial Ca2+-activated K+ channels
Authors: Luedders DW, Muenz BM, Li F, Rueckleben S, Tillmanns H, Waldecker B, Wiecha J, Erdogan A, Schaefer CA, Kuhlmann CR.
Journal: J Cardiovasc Pharmacol (2006): 365
Flow cytometry analysis of germinating Bacillus spores, using membrane potential dye
Authors: Laflamme C, Ho J, Veillette M, de Latremoille MC, Verreault D, Meriaux A, Duchaine C.
Journal: Arch Microbiol (2005): 107
K+ currents regulate the resting membrane potential, proliferation, and contractile responses in ventricular fibroblasts and myofibroblasts
Authors: Chilton L, Ohya S, Freed D, George E, Drobic V, Shibukawa Y, Maccannell KA, Imaizumi Y, Clark RB, Dixon IM, Giles WR.
Journal: Am J Physiol Heart Circ Physiol (2005): H2931
A new signaling mechanism of hepatocyte growth factor-induced endothelial proliferation
Authors: Kuhlmann CR, Schaefer CA, Fehsecke A, Most AK, Tillmanns H, Erdogan A.
Journal: J Thromb Haemost (2005): 2089
Margatoxin inhibits VEGF-induced hyperpolarization, proliferation and nitric oxide production of human endothelial cells
Authors: Erdogan A, Schaefer CA, Schaefer M, Luedders DW, Stockhausen F, Abdallah Y, Schaefer C, Most AK, Tillmanns H, Piper HM, Kuhlmann CR.
Journal: J Vasc Res (2005): 368
Quercetin-induced induction of the NO/cGMP pathway depends on Ca2+-activated K+ channel-induced hyperpolarization-mediated Ca2+-entry into cultured human endothelial cells
Authors: Kuhlmann CR, Schaefer CA, Kosok C, Abdallah Y, Walther S, Ludders DW, Neumann T, Tillmanns H, Schafer C, Piper HM, Erdogan A.
Journal: Planta Med (2005): 520