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DiBAC4(3) [Bis-(1,3-dibutylbarbituric acid)trimethine oxonol]

DiBAC4(3) is a sensitive slow-response probe for measuring cellular membrane potential. In general, slow-response probes exhibit potential-dependent changes in their transmembrane distribution that are accompanied by a fluorescence change. The magnitude of their optical responses is much larger than that of fast-response probes (typically a 1% fluorescence change per mV). Slow-response probes, which include cationic carbocyanines, rhodamines and anionic oxonols, are suitable for detecting changes in average membrane potentials of nonexcitable cells caused by respiratory activity, ion-channel permeability, drug binding and other factors.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiBAC4(3) [Bis-(1,3-dibutylbarbituric acid)trimethine oxonol] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM193.558 µL967.792 µL1.936 mL9.678 mL19.356 mL
5 mM38.712 µL193.558 µL387.117 µL1.936 mL3.871 mL
10 mM19.356 µL96.779 µL193.558 µL967.792 µL1.936 mL

Molarity calculator

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Spectrum

Citations

View all 18 citations: Citation Explorer
K2P2. 1 channels modulate the pH-and mechanosensitivity of pancreatic stellate cells
Authors: Rugi, Micol and Hofschr{\"o}er, Verena and Peth{\H{o}}, Zolt{\'a}n and Soret, Benjamin and Loeck, Thorsten and Schwab, Albrecht
Journal: Pfl{\"u}gers Archiv-European Journal of Physiology (2024): 1--11
Co-exposure to pentachlorophenol (PCP) and cadmium (Cd) triggers apoptosis-like cell death in Eschericia coli
Authors: Chen, Zhilan and Jiang, Yi and Lai, Xuebin and Zhu, Chenhong and Zhang, Dapeng and Wang, Hailin
Journal: Environmental Pollution (2024): 123640
Membrane Domain Anti-Registration Induces an Intrinsic Transmembrane Potential
Authors: Lin, Xiaoqian and Lin, Kaidong and He, Shiqi and Zhou, Yue and Li, Xiu and Lin, Xubo
Journal: Langmuir (2023)
Regulation of localized corrosion of 316L stainless steel on osteogenic differentiation of bone morrow derived mesenchymal stem cells
Authors: Li, Meng and Wu, Jing and Geng, Wenbo and Yang, Yulu and Li, Xuan and Xu, Kun and Li, Ke and Li, Yan and Duan, Qiaojian and Gao, Pengfei and others,
Journal: Biomaterials (2023): 122262
Effects of extremely low frequency electromagnetic fields on the tumor cell inhibition and the possible mechanism
Authors: Sun, Jie and Tong, Yingying and Jia, Yu and Jia, Xu and Wang, Hua and Chen, Yang and Wu, Jiamin and Jin, Weiyang and Ma, Zheng and Cao, Kai and others,
Journal: Scientific Reports (2023): 6989

References

View all 36 references: Citation Explorer
Activation of Ca(2+)-activated potassium channels is involved in lysophosphatidylcholine-induced monocyte adhesion to endothelial cells
Authors: Erdogan A, Schaefer MB, Kuhlmann CR, Most A, Hartmann M, Mayer K, Renner FC, Schaefer C, Abdallah Y, Hoelschermann H, Schaefer CA.
Journal: Atherosclerosis. (2006)
The Zn2+-transporting pathways in pancreatic beta-cells: a role for the L-type voltage-gated Ca2+ channel
Authors: Gyulkh, undefined and anyan AV, Lee SC, Bikopoulos G, Dai F, Wheeler MB.
Journal: J Biol Chem (2006): 9361
N-terminal parathyroid hormone-related peptide hyperpolarizes endothelial cells and causes a reduction of the coronary resistance of the rat heart via endothelial hyperpolarization
Authors: Abdallah Y, Ross G, Dolf A, Heinemann MP, Schluter KD.
Journal: Peptides (2006): 2927
Rapid assessment of the physiological status of Streptococcus macedonicus by flow cytometry and fluorescence probes
Authors: Papadimitriou K, Pratsinis H, Nebe-von-Caron G, Kletsas D, Tsakalidou E.
Journal: Int J Food Microbiol (2006): 197
Role of cGMP in sildenafil-induced activation of endothelial Ca2+-activated K+ channels
Authors: Luedders DW, Muenz BM, Li F, Rueckleben S, Tillmanns H, Waldecker B, Wiecha J, Erdogan A, Schaefer CA, Kuhlmann CR.
Journal: J Cardiovasc Pharmacol (2006): 365
Page updated on December 6, 2024

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Physical properties

Molecular weight

516.64

Solvent

DMSO

Spectral properties

Excitation (nm)

493

Emission (nm)

517

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200

CAS

70363-83-6
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Gallery Image 1
The effect of clioquinol in cell membrane. a) Using propidium iodide influx assay to evaluate the influence on plasma membrane integrity; b) Using DiBAC4(3) to evaluate the membrane potential. CQ increased DiBAC4(3) fluorescence intensity by 12.8, 23.8, 113.7, 124.2, 147.8 and 167.2% at different concentrations (1, 2, 4, 8, 16 and 32 μg/ml) compared with untreated cells, respectively. *, p < 0.05; **, p < 0.001; ***, p < 0.0001; DiBAC4(3), bis-(1,3-dibutylbarbituric acid) trimethine oxonol; CQ, clioquinol; AMB, amphotericin B. Source: <b>The effects of clioquinol in morphogenesis, cell membrane and ion homeostasis in <em>Candida albicans</em></b> by Zimeng You, Chaoliang Zhang & Yuping Ran. <em>BMC Microbiology</em>, June 2020.