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DiBAC4(3) [Bis-(1,3-dibutylbarbituric acid)trimethine oxonol]
DiBAC4(3) is a sensitive slow-response probe for measuring cellular membrane potential. In general, slow-response probes exhibit potential-dependent changes in their transmembrane distribution that are accompanied by a fluorescence change. The magnitude of their optical responses is much larger than that of fast-response probes (typically a 1% fluorescence change per mV). Slow-response probes, which include cationic carbocyanines, rhodamines and anionic oxonols, are suitable for detecting changes in average membrane potentials of nonexcitable cells caused by respiratory activity, ion-channel permeability, drug binding and other factors.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiBAC4(3) [Bis-(1,3-dibutylbarbituric acid)trimethine oxonol] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 193.558 µL | 967.792 µL | 1.936 mL | 9.678 mL | 19.356 mL |
| 5 mM | 38.712 µL | 193.558 µL | 387.117 µL | 1.936 mL | 3.871 mL |
| 10 mM | 19.356 µL | 96.779 µL | 193.558 µL | 967.792 µL | 1.936 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| DiBAC4(5) [Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol] | 591 | 615 |
| DiSBAC2(3) [Bis-(1,3-diethylthiobarbituric acid)trimethine oxonol] | 538 | 555 |
Citations
View all 19 citations: Citation Explorer
Deciphering the metabolic details of L-lysine toxicity in cyanobacteria
Authors: Enkerlin, Andreas M and Hoffmann, Uta A and Rapp, Johanna and Miao, Rui and Link, Hannes and Hudson, Elton P and Selim, Khaled A
Journal: bioRxiv (2025): 2025--03
Authors: Enkerlin, Andreas M and Hoffmann, Uta A and Rapp, Johanna and Miao, Rui and Link, Hannes and Hudson, Elton P and Selim, Khaled A
Journal: bioRxiv (2025): 2025--03
K2P2. 1 channels modulate the pH-and mechanosensitivity of pancreatic stellate cells
Authors: Rugi, Micol and Hofschr{\"o}er, Verena and Peth{\H{o}}, Zolt{\'a}n and Soret, Benjamin and Loeck, Thorsten and Schwab, Albrecht
Journal: Pfl{\"u}gers Archiv-European Journal of Physiology (2024): 1--11
Authors: Rugi, Micol and Hofschr{\"o}er, Verena and Peth{\H{o}}, Zolt{\'a}n and Soret, Benjamin and Loeck, Thorsten and Schwab, Albrecht
Journal: Pfl{\"u}gers Archiv-European Journal of Physiology (2024): 1--11
Co-exposure to pentachlorophenol (PCP) and cadmium (Cd) triggers apoptosis-like cell death in Eschericia coli
Authors: Chen, Zhilan and Jiang, Yi and Lai, Xuebin and Zhu, Chenhong and Zhang, Dapeng and Wang, Hailin
Journal: Environmental Pollution (2024): 123640
Authors: Chen, Zhilan and Jiang, Yi and Lai, Xuebin and Zhu, Chenhong and Zhang, Dapeng and Wang, Hailin
Journal: Environmental Pollution (2024): 123640
Membrane Domain Anti-Registration Induces an Intrinsic Transmembrane Potential
Authors: Lin, Xiaoqian and Lin, Kaidong and He, Shiqi and Zhou, Yue and Li, Xiu and Lin, Xubo
Journal: Langmuir (2023)
Authors: Lin, Xiaoqian and Lin, Kaidong and He, Shiqi and Zhou, Yue and Li, Xiu and Lin, Xubo
Journal: Langmuir (2023)
Regulation of localized corrosion of 316L stainless steel on osteogenic differentiation of bone morrow derived mesenchymal stem cells
Authors: Li, Meng and Wu, Jing and Geng, Wenbo and Yang, Yulu and Li, Xuan and Xu, Kun and Li, Ke and Li, Yan and Duan, Qiaojian and Gao, Pengfei and others,
Journal: Biomaterials (2023): 122262
Authors: Li, Meng and Wu, Jing and Geng, Wenbo and Yang, Yulu and Li, Xuan and Xu, Kun and Li, Ke and Li, Yan and Duan, Qiaojian and Gao, Pengfei and others,
Journal: Biomaterials (2023): 122262
References
View all 36 references: Citation Explorer
Activation of Ca(2+)-activated potassium channels is involved in lysophosphatidylcholine-induced monocyte adhesion to endothelial cells
Authors: Erdogan A, Schaefer MB, Kuhlmann CR, Most A, Hartmann M, Mayer K, Renner FC, Schaefer C, Abdallah Y, Hoelschermann H, Schaefer CA.
Journal: Atherosclerosis. (2006)
Authors: Erdogan A, Schaefer MB, Kuhlmann CR, Most A, Hartmann M, Mayer K, Renner FC, Schaefer C, Abdallah Y, Hoelschermann H, Schaefer CA.
Journal: Atherosclerosis. (2006)
The Zn2+-transporting pathways in pancreatic beta-cells: a role for the L-type voltage-gated Ca2+ channel
Authors: Gyulkh, undefined and anyan AV, Lee SC, Bikopoulos G, Dai F, Wheeler MB.
Journal: J Biol Chem (2006): 9361
Authors: Gyulkh, undefined and anyan AV, Lee SC, Bikopoulos G, Dai F, Wheeler MB.
Journal: J Biol Chem (2006): 9361
N-terminal parathyroid hormone-related peptide hyperpolarizes endothelial cells and causes a reduction of the coronary resistance of the rat heart via endothelial hyperpolarization
Authors: Abdallah Y, Ross G, Dolf A, Heinemann MP, Schluter KD.
Journal: Peptides (2006): 2927
Authors: Abdallah Y, Ross G, Dolf A, Heinemann MP, Schluter KD.
Journal: Peptides (2006): 2927
Rapid assessment of the physiological status of Streptococcus macedonicus by flow cytometry and fluorescence probes
Authors: Papadimitriou K, Pratsinis H, Nebe-von-Caron G, Kletsas D, Tsakalidou E.
Journal: Int J Food Microbiol (2006): 197
Authors: Papadimitriou K, Pratsinis H, Nebe-von-Caron G, Kletsas D, Tsakalidou E.
Journal: Int J Food Microbiol (2006): 197
Role of cGMP in sildenafil-induced activation of endothelial Ca2+-activated K+ channels
Authors: Luedders DW, Muenz BM, Li F, Rueckleben S, Tillmanns H, Waldecker B, Wiecha J, Erdogan A, Schaefer CA, Kuhlmann CR.
Journal: J Cardiovasc Pharmacol (2006): 365
Authors: Luedders DW, Muenz BM, Li F, Rueckleben S, Tillmanns H, Waldecker B, Wiecha J, Erdogan A, Schaefer CA, Kuhlmann CR.
Journal: J Cardiovasc Pharmacol (2006): 365
Page updated on September 2, 2025
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