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DiSBAC2(3) [Bis-(1,3-diethylthiobarbituric acid)trimethine oxonol]

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Physical properties
Molecular weight436.55
Spectral properties
Excitation (nm)538
Emission (nm)555
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
DiSBAC2(3) is a sensitive slow-response membrane potential probe that is widely used for measuring membrane potentials of many biological systems. In general, slow-response probes exhibit potential-dependent changes in their transmembrane distribution that are accompanied by a fluorescence change. The magnitude of their optical responses is much larger than that of fast-response probes (typically a 1% fluorescence change per mV). Slow-response probes, which include cationic carbocyanines, rhodamines and anionic oxonols, are suitable for detecting changes in average membrane potentials of nonexcitable cells caused by respiratory activity, ion-channel permeability, drug binding and other factors.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiSBAC2(3) [Bis-(1,3-diethylthiobarbituric acid)trimethine oxonol] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM229.069 µL1.145 mL2.291 mL11.453 mL22.907 mL
5 mM45.814 µL229.069 µL458.138 µL2.291 mL4.581 mL
10 mM22.907 µL114.534 µL229.069 µL1.145 mL2.291 mL

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Spectral properties

Excitation (nm)538
Emission (nm)555



View all 3 citations: Citation Explorer
Role of 4-hydroxybutyrate in increased resistance to surgical site infections associated with surgical meshes
Authors: Molina, Catalina Pineda and Hussey, George S and Liu, Alvin and Eriksson, Jonas and D'Angelo, William A and Badylak, Stephen F
Journal: Biomaterials (2021): 120493
Src family kinases-mediated negative regulation of sperm acrosome reaction in chickens (Gallus gallus domesticus)
Authors: Priyadarshana, Chathura and Setiawan, Rangga and Tajima, Atsushi and Asano, Atsushi
Journal: PloS one (2020): e0241181
Suppression of K V 7/KCNQ potassium channel enhances neuronal differentiation of PC12 cells
Authors: Zhou, Najing and Huang, Sha and Li, Li and Huang, Dongyang and Yan, Yunli and Du, Xiaona and Zhang, Hailin
Journal: Neuroscience (2016): 356--367


View all 3 references: Citation Explorer
Comparative study of membrane potential-sensitive fluorescent probes and their use in ion channel screening assays
Authors: Wolff C, Fuks B, Chatelain P.
Journal: J Biomol Screen (2003): 533
Characterization of the taurine transport pathway in A6 kidney cells
Authors: Schmieder S, Soriani O, Brochiero E, Raschi C, Bogliolo S, Lindenthal S, Ehrenfeld J.
Journal: J Membr Biol (2002): 145
Membrane potential changes after infection of monocytes by Toxoplasma gondii
Authors: Bouchot A, Millot JM, Charpentier S, Bonhomme A, Villena I, Aubert D, Pinon JM.
Journal: Int J Parasitol (2001): 1114