DiBAC4(5) [Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol]

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Physical properties

Molecular weight	542.67
Solvent	DMSO

Spectral properties

Excitation (nm)	591
Emission (nm)	615

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

CAS

63560-89-4

Molecular weight

542.67

Excitation (nm)

591

Emission (nm)

615

DiBAC4(5) is a sensitive membrane potential probe with longer excitation and emission wavelength. It is a slow-response probe for measuring cellular membrane potential. In general, slow-response probes exhibit potential-dependent changes in their transmembrane distribution that are accompanied by a fluorescence change. The magnitude of their optical responses is much larger than that of fast-response probes (typically a 1% fluorescence change per mV). Slow-response probes, which include cationic carbocyanines, rhodamines and anionic oxonols, are suitable for detecting changes in average membrane potentials of nonexcitable cells caused by respiratory activity, ion-channel permeability, drug binding and other factors.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiBAC4(5) [Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	184.274 µL	921.37 µL	1.843 mL	9.214 mL	18.427 mL
5 mM	36.855 µL	184.274 µL	368.548 µL	1.843 mL	3.685 mL
10 mM	18.427 µL	92.137 µL	184.274 µL	921.37 µL	1.843 mL

Molarity calculator

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Spectrum

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Spectral properties

Excitation (nm)	591
Emission (nm)	615

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
5-FAM [5-Carboxyfluorescein] CAS 76823-03-5	493	517	83000	0.178
5-SFX [5-FAM-X, SE] CAS 148356-00-7	493	517	83000	0.178
5-FITC [Fluorescein-5-isothiocyanate] CAS 3326-32-7	491	516	73000	0.35
5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] CAS 51306-35-5	498	517	80000¹	0.35
5-IAF [5-Iodoacetamidofluorescein] CAS 63368-54-7	498	517	80000¹	0.35
5-CR110 [5-Carboxyrhodamine 110] Single isomer	499	525	84000	0.091
5-CR6G [5-Carboxyrhodamine 6G] Single isomer	522	546	94000	0.214
5-TAMRA [5-Carboxytetramethylrhodamine] CAS 91809-66-4	552	578	90000	0.178
5-ROX [5-Carboxy-X-rhodamine] CAS 216699-35-3	578	604	82000	0.168
5-TRITC [Tetramethylrhodamine-5-isothiocyanate] CAS 80724-19-2	544	570	100000	0.34
DiBAC4(3) [Bis-(1,3-dibutylbarbituric acid)trimethine oxonol]	493	517	-	-
DiSBAC2(5) [Bis-(1,3-diethylthiobarbituric acid)pentamethine oxonol]	636	655	-	-
Show More (3)

Images

Figure 1. Chemical structure for DiBAC4(5) [Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol]

Citations

View all 5 citations: Citation Explorer

A method for quantifying sporulation efficiency and isolating meiotic progeny in non-GMO strains of Saccharomyces cerevisiae
Authors: Sirr, Amy and Timour, Martin S and Cromie, Gareth A and Tang, Michelle and Dudley, Aim{\'e}e M
Journal: Yeast (2022): 354--362

A Membrane Potential-and Calpain-Dependent Reversal of Caspase-1 Inhibition Regulates Canonical NLRP3 Inflammasome
Authors: Zhang, Yifei and Rong, Hua and Zhang, Fang-Xiong and Wu, Kun and Mu, Libing and Meng, Junchen and Xiao, Bailong and Zamponi, Gerald W and Shi, Yan
Journal: Cell reports (2018): 2356--2369

Effects of 1, 4-naphthoquinone Aged Carbon Black Particles on the Cell Membrane of Human Bronchial Epithelium
Authors: Zhang, Yongming and Wang, Li and Feng, Huimin and Hu, Guiping and Wang, Lele and Liu, Jiaxing and Gao, Xin and Shang, Jing and Zhu, Tong and Tang, Shichuan and others, undefined
Journal: Environmental Toxicology and Pharmacology (2017)

Humic analog AQDS can act as a selective inhibitor to enable anoxygenic photosynthetic bacteria to outcompete sulfate-reducing bacteria under microaerobic conditions
Authors: Wang, Xingzu and Cheng, Xiang and Ren, Yiwei and Xu, Guihua and Tang, Jing
Journal: Journal of Chemical Technology and Biotechnology (2015)

Mitochondrial ROS-K+ channel signaling pathway regulated secretion of human pulmonary artery endothelial cells
Authors: Ouyang, Jin-Sheng and Li, Yu-Ping and Li, Cheng-Ye and Cai, Chang and Chen, Cheng-Shui and Chen, Shao-Xian and Chen, Yan-Fan and Yang, Li and Xie, Yu-Peng
Journal: Free radical research (2012): 1437--1445

References

View all 36 references: Citation Explorer

Activation of Ca(2+)-activated potassium channels is involved in lysophosphatidylcholine-induced monocyte adhesion to endothelial cells
Authors: Erdogan A, Schaefer MB, Kuhlmann CR, Most A, Hartmann M, Mayer K, Renner FC, Schaefer C, Abdallah Y, Hoelschermann H, Schaefer CA.
Journal: Atherosclerosis. (2006)

The Zn2+-transporting pathways in pancreatic beta-cells: a role for the L-type voltage-gated Ca2+ channel
Authors: Gyulkh, undefined and anyan AV, Lee SC, Bikopoulos G, Dai F, Wheeler MB.
Journal: J Biol Chem (2006): 9361

N-terminal parathyroid hormone-related peptide hyperpolarizes endothelial cells and causes a reduction of the coronary resistance of the rat heart via endothelial hyperpolarization
Authors: Abdallah Y, Ross G, Dolf A, Heinemann MP, Schluter KD.
Journal: Peptides (2006): 2927

Rapid assessment of the physiological status of Streptococcus macedonicus by flow cytometry and fluorescence probes
Authors: Papadimitriou K, Pratsinis H, Nebe-von-Caron G, Kletsas D, Tsakalidou E.
Journal: Int J Food Microbiol (2006): 197

Role of cGMP in sildenafil-induced activation of endothelial Ca2+-activated K+ channels
Authors: Luedders DW, Muenz BM, Li F, Rueckleben S, Tillmanns H, Waldecker B, Wiecha J, Erdogan A, Schaefer CA, Kuhlmann CR.
Journal: J Cardiovasc Pharmacol (2006): 365

K+ currents regulate the resting membrane potential, proliferation, and contractile responses in ventricular fibroblasts and myofibroblasts
Authors: Chilton L, Ohya S, Freed D, George E, Drobic V, Shibukawa Y, Maccannell KA, Imaizumi Y, Clark RB, Dixon IM, Giles WR.
Journal: Am J Physiol Heart Circ Physiol (2005): H2931

A new signaling mechanism of hepatocyte growth factor-induced endothelial proliferation
Authors: Kuhlmann CR, Schaefer CA, Fehsecke A, Most AK, Tillmanns H, Erdogan A.
Journal: J Thromb Haemost (2005): 2089

Margatoxin inhibits VEGF-induced hyperpolarization, proliferation and nitric oxide production of human endothelial cells
Authors: Erdogan A, Schaefer CA, Schaefer M, Luedders DW, Stockhausen F, Abdallah Y, Schaefer C, Most AK, Tillmanns H, Piper HM, Kuhlmann CR.
Journal: J Vasc Res (2005): 368

Quercetin-induced induction of the NO/cGMP pathway depends on Ca2+-activated K+ channel-induced hyperpolarization-mediated Ca2+-entry into cultured human endothelial cells
Authors: Kuhlmann CR, Schaefer CA, Kosok C, Abdallah Y, Walther S, Ludders DW, Neumann T, Tillmanns H, Schafer C, Piper HM, Erdogan A.
Journal: Planta Med (2005): 520

Flow cytometry analysis of germinating Bacillus spores, using membrane potential dye
Authors: Laflamme C, Ho J, Veillette M, de Latremoille MC, Verreault D, Meriaux A, Duchaine C.
Journal: Arch Microbiol (2005): 107