dTTP 100 mM PCR Grade

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Physical properties

Molecular weight	482.17
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

365-08-2

Molecular weight

482.17

2'-Deoxythymidine-5'-Triphosphate (dTTP) solution is used in DNA synthesis reactions such as PCR, DNA sequencing and molecular cloning techniques. This dATP solution is suitable for many applications where high-quality reagents are required. Such procedures include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of dTTP *100 mM PCR Grade* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	207.396 µL	1.037 mL	2.074 mL	10.37 mL	20.74 mL
5 mM	41.479 µL	207.396 µL	414.791 µL	2.074 mL	4.148 mL
10 mM	20.74 µL	103.698 µL	207.396 µL	1.037 mL	2.074 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Images

Figure 1. Chemical structure for dTTP *100 mM PCR Grade*.

References

View all 50 references: Citation Explorer

Enzymatic Synthesis of Chimeric DNA Oligonucleotides by in Vitro Transcription with dTTP, dCTP, dATP, and 2'-Fluoro Modified dGTP.
Authors: Kapustina, Žana and Jasponė, Aistė and Dubovskaja, Varvara and Mackevičius, Gytis and Lubys, Arvydas
Journal: ACS synthetic biology (2021): 1625-1632

Analysis of Modified Nucleotide Aptamer Library Generated by Thermophilic DNA Polymerases.
Authors: Percze, Krisztina and Mészáros, Tamás
Journal: Chembiochem : a European journal of chemical biology (2020): 2939-2944

HDACi mediate UNG2 depletion, dysregulated genomic uracil and altered expression of oncoproteins and tumor suppressors in B- and T-cell lines.
Authors: Iveland, Tobias S and Hagen, Lars and Sharma, Animesh and Sousa, Mirta M L and Sarno, Antonio and Wollen, Kristian Lied and Liabakk, Nina Beate and Slupphaug, Geir
Journal: Journal of translational medicine (2020): 159

Polymerase synthesis of four-base DNA from two stable dimeric nucleotides.
Authors: Mohsen, Michael G and Ji, Debin and Kool, Eric T
Journal: Nucleic acids research (2019): 9495-9501

Preamplification with dUTP and Cod UNG Enables Elimination of Contaminating Amplicons.
Authors: Andersson, Daniel and Svec, David and Pedersen, Cathrine and Henriksen, Jørn Remi and Ståhlberg, Anders
Journal: International journal of molecular sciences (2018)

Point of sampling detection of Zika virus within a multiplexed kit capable of detecting dengue and chikungunya.
Authors: Yaren, Ozlem and Alto, Barry W and Gangodkar, Priyanka V and Ranade, Shatakshi R and Patil, Kunal N and Bradley, Kevin M and Yang, Zunyi and Phadke, Nikhil and Benner, Steven A
Journal: BMC infectious diseases (2017): 293

Minimizing carry-over PCR contamination in expanded CAG/CTG repeat instability applications.
Authors: Aeschbach, Lorène and Dion, Vincent
Journal: Scientific reports (2017): 18026

Efficient enzymatic synthesis and dual-colour fluorescent labelling of DNA probes using long chain azido-dUTP and BCN dyes.
Authors: Ren, Xiaomei and El-Sagheer, Afaf H and Brown, Tom
Journal: Nucleic acids research (2016): e79

Post-synthesis DNA modifications using a trans-cyclooctene click handle.
Authors: Wang, Ke and Wang, Danzhu and Ji, Kaili and Chen, Weixuan and Zheng, Yueqin and Dai, Chaofeng and Wang, Binghe
Journal: Organic & biomolecular chemistry (2015): 909-15

Molecular identification of Anisakis and Hysterothylacium larvae in marine fishes from the East China Sea and the Pacific coast of central Japan.
Authors: Kong, Qingming and Fan, Lanfen and Zhang, Junhe and Akao, Nobuaki and Dong, Kewei and Lou, Di and Ding, Jianzu and Tong, Qunbo and Zheng, Bin and Chen, Rui and Ohta, Nobuo and Lu, Shaohong
Journal: International journal of food microbiology (2015): 1-7