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DY547 CEP [DyLight 547 phosphoramidite]
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 756.75 |
| Solvent | MeCN |
Spectral properties
| Extinction coefficient (cm -1 M -1) | 1500001 |
| Excitation (nm) | 558 |
| Emission (nm) | 574 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
Molecular weight 756.75 | Extinction coefficient (cm -1 M -1) 1500001 | Excitation (nm) 558 | Emission (nm) 574 |
DY547 CEP (DyLight 547 phosphoramidite) is an excellent replacement to the commonly used Cy3 phosphoramidite. Two cyanine derivatives, Cy3 and Cy5 phosphoramidites, are the most popular fluorescent labeling reagents for preparing fluorescence-labeled oligonucleotides. However, both Cy3 and Cy5 phosphoramidites are not very stable. DY547 CEP appears to be more stable than Cy3 phosphoramidite. As Cy3 and Cy5 phosphoramidites, the deprotection of oligos containing DY547 CEP may be carried out with ammonium hydroxide at room temperature, regardless of the base protecting groups on the monomers used. However, it is always prudent to use monomers with base labile protecting groups to limit the exposure time to 2 hours or less at 65°C during deprotection.
Calculators
Common stock solution preparation
Table 1. Volume of MeCN needed to reconstitute specific mass of DY547 CEP [DyLight 547 phosphoramidite] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 132.144 µL | 660.72 µL | 1.321 mL | 6.607 mL | 13.214 mL |
| 5 mM | 26.429 µL | 132.144 µL | 264.288 µL | 1.321 mL | 2.643 mL |
| 10 mM | 13.214 µL | 66.072 µL | 132.144 µL | 660.72 µL | 1.321 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Extinction coefficient (cm -1 M -1) | 1500001 |
| Excitation (nm) | 558 |
| Emission (nm) | 574 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| DY647 CEP [DyLight 647 phosphoramidite] | 652 | 673 | 2500001 |
Images
Figure 1. Chemical structure for DY547 CEP [DyLight 547 phosphoramidite].
References
View all 50 references: Citation Explorer
Probing functional conformation-state fluctuation dynamics in recognition binding between calmodulin and target peptide.
Authors: Jaiswal, Sunidhi and He, Yufan and Lu, H Peter
Journal: The Journal of chemical physics (2022): 055102
Authors: Jaiswal, Sunidhi and He, Yufan and Lu, H Peter
Journal: The Journal of chemical physics (2022): 055102
Insulin-Like Growth Factor-1 Receptor Targeted Fluorescent Imaging for Gallbladder Cancer in Orthotopic Mouse Models.
Authors: Choi, Jung Ha and Park, Jeong Youp
Journal: Gut and liver (2021)
Authors: Choi, Jung Ha and Park, Jeong Youp
Journal: Gut and liver (2021)
Stability Enhancement of a Dimeric HER2-Specific Affibody Molecule through Sortase A-Catalyzed Head-to-Tail Cyclization.
Authors: Westerlund, Kristina and Myrhammar, Anders and Tano, Hanna and Gestin, Maxime and Karlström, Amelie Eriksson
Journal: Molecules (Basel, Switzerland) (2021)
Authors: Westerlund, Kristina and Myrhammar, Anders and Tano, Hanna and Gestin, Maxime and Karlström, Amelie Eriksson
Journal: Molecules (Basel, Switzerland) (2021)
Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems.
Authors: Stech, Marlitt and Rakotoarinoro, Nathanaël and Teichmann, Tamara and Zemella, Anne and Thoring, Lena and Kubick, Stefan
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 175-190
Authors: Stech, Marlitt and Rakotoarinoro, Nathanaël and Teichmann, Tamara and Zemella, Anne and Thoring, Lena and Kubick, Stefan
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 175-190
Novel EBV LMP1 C-terminal domain binding affibody molecules as potential agents for in vivo molecular imaging diagnosis of nasopharyngeal carcinoma.
Authors: Kamara, Saidu and Guo, Yanru and Mao, Shanshan and Ye, Xiaoxian and Li, Qingfeng and Zheng, Maolin and Zhu, Jinshun and Zhang, Jing and Du, Wangqi and Chen, Jun and Zhu, Shanli and Zhang, Lifang
Journal: Applied microbiology and biotechnology (2021): 7283-7293
Authors: Kamara, Saidu and Guo, Yanru and Mao, Shanshan and Ye, Xiaoxian and Li, Qingfeng and Zheng, Maolin and Zhu, Jinshun and Zhang, Jing and Du, Wangqi and Chen, Jun and Zhu, Shanli and Zhang, Lifang
Journal: Applied microbiology and biotechnology (2021): 7283-7293
Simple methodology to visualize whole-brain microvasculature in three dimensions.
Authors: Khouri, Katiana and Xie, Danny F and Crouzet, Christian and Bahani, Adrian W and Cribbs, David H and Fisher, Mark J and Choi, Bernard
Journal: Neurophotonics (2021): 025004
Authors: Khouri, Katiana and Xie, Danny F and Crouzet, Christian and Bahani, Adrian W and Cribbs, David H and Fisher, Mark J and Choi, Bernard
Journal: Neurophotonics (2021): 025004
Visualization of Mouse Choroidal and Retinal Vasculature Using Fluorescent Tomato Lectin Perfusion.
Authors: Jiao, Chunhua and Adler, Kelsey and Liu, Xiuying and Sun, Weize and Mullins, Robert F and Sohn, Elliott H
Journal: Translational vision science & technology (2020): 1
Authors: Jiao, Chunhua and Adler, Kelsey and Liu, Xiuying and Sun, Weize and Mullins, Robert F and Sohn, Elliott H
Journal: Translational vision science & technology (2020): 1
Bacteriophage Tail-Spike Proteins Enable Detection of Pseudaminic-Acid-Coated Pathogenic Bacteria and Guide the Development of Antiglycan Antibodies with Cross-Species Antibacterial Activity.
Authors: Lee, I-Ming and Tu, I-Fan and Yang, Feng-Ling and Wu, Shih-Hsiung
Journal: Journal of the American Chemical Society (2020): 19446-19450
Authors: Lee, I-Ming and Tu, I-Fan and Yang, Feng-Ling and Wu, Shih-Hsiung
Journal: Journal of the American Chemical Society (2020): 19446-19450
High throughput pSTAT signaling profiling by fluorescent cell barcoding and computational analysis.
Authors: Tsai, Wanxia Li and Vian, Laura and Giudice, Valentina and Kieltyka, Jacqueline and Liu, Christine and Fonseca, Victoria and Gazaniga, Nathalia and Gao, Shouguo and Kajigaya, Sachiko and Young, Neal S and Biancotto, Angélique and Gadina, Massimo
Journal: Journal of immunological methods (2020): 112667
Authors: Tsai, Wanxia Li and Vian, Laura and Giudice, Valentina and Kieltyka, Jacqueline and Liu, Christine and Fonseca, Victoria and Gazaniga, Nathalia and Gao, Shouguo and Kajigaya, Sachiko and Young, Neal S and Biancotto, Angélique and Gadina, Massimo
Journal: Journal of immunological methods (2020): 112667
Bifunctional Microbead Carriers with Magnetic Response and Plasmonic Fluorescence Enhancement.
Authors: Sheng, Tao and Guo, Fanglei and Deng, Jingzhe and Yuan, Yi and Deng, Dawei and Xie, Zhuoying
Journal: Journal of nanoscience and nanotechnology (2019): 691-696
Authors: Sheng, Tao and Guo, Fanglei and Deng, Jingzhe and Yuan, Yi and Deng, Dawei and Xie, Zhuoying
Journal: Journal of nanoscience and nanotechnology (2019): 691-696