DY677 CEP [DyLight 677 phosphoramidite]

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Fax	1-800-609-2943
Email	sales@aatbio.com
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Physical properties

Molecular weight	927.20
Solvent	MeCN

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

927.20

DY677 CEP (DyLight 677 phosphoramidite) is an excellent replacement to the commonly used Cy5.5 phosphoramidite. Cyanine phosphoramidites (such as Cy3 and Cy5 phosphoramidites) are the most popular fluorescent labeling reagents for preparing fluorescence-labeled oligonucleotides. However, both Cy3 and Cy5 phosphoramidites are not very stable. DY677 CEP appears to be more stable than Cy5.5 phosphoramidite. As Cy5.5 phosphoramidite, the deprotection of oligos containing DY677 CEP may be carried out with ammonium hydroxide at room temperature, regardless of the base protecting groups on the monomers used. However, it is always prudent to use monomers with base labile protecting groups to limit the exposure time to 2 hours or less at 65°C during deprotection.

Calculators

Common stock solution preparation

Table 1. Volume of MeCN needed to reconstitute specific mass of DY677 CEP [DyLight 677 phosphoramidite] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	107.852 µL	539.258 µL	1.079 mL	5.393 mL	10.785 mL
5 mM	21.57 µL	107.852 µL	215.703 µL	1.079 mL	2.157 mL
10 mM	10.785 µL	53.926 µL	107.852 µL	539.258 µL	1.079 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
DY647 CEP [DyLight 647 phosphoramidite]	652	673	250000¹
DY547 CEP [DyLight 547 phosphoramidite]	558	574	150000¹

Images

Figure 1. Chemical structure for DY677 CEP [DyLight 677 phosphoramidite].

References

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Journal: Journal of immunological methods (2020): 112667

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