EDANS sodium salt [5-((2-Aminoethyl)aminonaphthalene-1-sulfonic acid, sodium salt] *CAS 100900-07-0*
Ordering information
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
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| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 288.30 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (280 nm) | 0.107 |
| Extinction coefficient (cm -1 M -1) | 5900 |
| Excitation (nm) | 336 |
| Emission (nm) | 455 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
See also: Digital PCR, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), RNA Purification & Analysis, PCR Detection of Viral DNA/RNA
CAS 100900-07-0 | Molecular weight 288.30 | Correction Factor (280 nm) 0.107 | Extinction coefficient (cm -1 M -1) 5900 | Excitation (nm) 336 | Emission (nm) 455 |
EDANS sodium salt is much more water-soluble than EDANS acid. It is one of the most popular donors for developing FRET-based nucleic acid probes (such as Molecular Beacons) and protease substrates. EDANS is often paired with DABCYL or DABSYL in FRET-based probes. Its fluorescence is environment-sensitive.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of EDANS sodium salt [5-((2-Aminoethyl)aminonaphthalene-1-sulfonic acid, sodium salt] *CAS 100900-07-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 346.861 µL | 1.734 mL | 3.469 mL | 17.343 mL | 34.686 mL |
| 5 mM | 69.372 µL | 346.861 µL | 693.722 µL | 3.469 mL | 6.937 mL |
| 10 mM | 34.686 µL | 173.43 µL | 346.861 µL | 1.734 mL | 3.469 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (280 nm) | 0.107 |
| Extinction coefficient (cm -1 M -1) | 5900 |
| Excitation (nm) | 336 |
| Emission (nm) | 455 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
| Resorufin, sodium salt *CAS 34994-50-8* | 571 | 584 | 650001 | 0.751 |
| Resazurin, sodium salt *CAS 62758-13-8* | 571 | 584 | - | - |
Images
Figure 1. Chemical structure for EDANS sodium salt [5-((2-Aminoethyl)aminonaphthalene-1-sulfonic acid, sodium salt] *CAS 100900-07-0*
References
View all 54 references: Citation Explorer
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Journal: Chembiochem (2005): 1043
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Journal: J Biol Chem (2004): 20775