FAM Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	N/A
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
TAMRA Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well	552	578	90000
VIC Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well	526	543	-
ROX Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well	578	604	82000
SYBR Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well	498	522	-
NED Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well	545	567	74000¹
JOE Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well	520	545	75000¹
FAM Dye qPCR Calibration Solution 10,000X	493	517	83000
Cy5 Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well	651	670	250000¹

Images

Figure 1. ABI 7500 FAST system FAM dye spectrum

References

View all 50 references: Citation Explorer

Novel design of nucleic acid standards for hydrolysis probe-based PCR with melting analysis.
Authors: Baoutina, Anna and Bhat, Somanath
Journal: Gene therapy (2021)

Determination of Advantages and Limitations of qPCR Duplexing in a Single Fluorescent Channel.
Authors: Zhang, Haoqing and Yan, Zhiqiang and Wang, Xinlu and Gaňová, Martina and Chang, Honglong and Laššáková, Soňa and Korabecna, Marie and Neuzil, Pavel
Journal: ACS omega (2021): 22292-22300

SARS-CoV-2 Detection using Real Time PCR by a Commercial Diagnostic Kit.
Authors: Roy, S and Paul, S K and Barman, T K and Ahmed, S and Haque, N and Mazid, R and Debnath, P and Roy, S A
Journal: Mymensingh medical journal : MMJ (2020): 596-600

Fluorescent Molecular Beacons Mimicking RNA Secondary Structures to Study RNA Chaperone Activity.
Authors: Menendez-Gil, Pilar and Caballero, Carlos J and Solano, Cristina and Toledo-Arana, Alejandro
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 41-58

Development and Evaluation of a Single Dye Duplex Droplet Digital PCR Assay for the Rapid Detection and Quantification of Mycobacterium tuberculosis.
Authors: Nyaruaba, Raphael and Xiong, Jin and Mwaliko, Caroline and Wang, Nuo and Kibii, Belindah J and Yu, Junping and Wei, Hongping
Journal: Microorganisms (2020)

A handheld continuous-flow real-time fluorescence qPCR system with a PVC microreactor.
Authors: Shi, Bing and Li, Yuanming and Wu, Di and Wu, Wenming
Journal: The Analyst (2020): 2767-2773

Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions.
Authors: Tomé, Stéphanie and Gourdon, Geneviève
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 11-23

Ratiometric fluorescence sensor based on carbon dots as internal reference signal and T7 exonuclease-assisted signal amplification strategy for microRNA-21 detection.
Authors: Wang, Zhenzhen and Xue, Zhiqiang and Hao, Xiaoli and Miao, Chenfang and Zhang, Jianzhong and Zheng, Yanjie and Zheng, Zongfu and Lin, Xinhua and Weng, Shaohuang
Journal: Analytica chimica acta (2020): 212-219

PharmFrag: An Easy and Fast Multiplex Pharmacogenetics Assay to Simultaneously Analyze 9 Genetic Polymorphisms Involved in Response Variability of Anticancer Drugs.
Authors: Bouvet, Régis and Verdier, Marie-Clémence and El Baroudi, Yahya and Galibert, Marie-Dominique and David, Véronique and Schutz, Sacha and Tron, Camille
Journal: International journal of molecular sciences (2020)

The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR.
Authors: Liu, Jinding and Wang, Rongshuai and Shi, Jie and Cheng, Xiaojuan and Hao, Ting and Guo, Jiangling and Wang, Jiaqi and Liu, Zidong and Li, Wenyan and Fan, Haoliang and Yun, Keming and Yan, Jiangwei and Zhang, Gengqian
Journal: International journal of legal medicine (2020): 2015-2027