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FAM Dye qPCR Calibration Solution *10,000X*
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
| Solvent | Water |
| Correction Factor (260 nm) | 0.32 |
| Correction Factor (280 nm) | 0.178 |
| Extinction coefficient (cm -1 M -1) | 83000 |
| Excitation (nm) | 493 |
| Emission (nm) | 517 |
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| Overview |  SDSProtocol |
Correction Factor (260 nm) 0.32 | Correction Factor (280 nm) 0.178 | Extinction coefficient (cm -1 M -1) 83000 | Excitation (nm) 493 | Emission (nm) 517 |
Platform
qPCR
| Recommended plate | PCR Microplate |
| Instrument specification(s) | FAM Filter |
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
This generic protocol is suitable for the ABI7500 FAST qPCR instrument. For complete instructions on use, refer to the maintenance guide for your instrument.
Prepare a FAM Dye qPCR Calibration working solution by mixing 1 µL of the 10,000X FAM Dye qPCR Calibration Solution with 10 mL of nuclease-free water or qPCR buffer.
Note: The final dye concentration must be optimized based on the calibration requirements for your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.
Add 20 µL of the FAM Dye qPCR Calibration working solution to all wells of a reaction plate.
Note: The volume may vary depending on your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.
Seal the plate with an optical adhesive cover.
Centrifuge the plate for 2 minutes at < 1500 rpm.
Check that the liquid in each well of the plate is at the bottom of the well. If not, centrifuge the plate again at a slightly higher rpm and for a longer period of time.
For complete calibration instructions, refer to the maintenance guide for your instrument.
Spectrum
Spectral properties
| Correction Factor (260 nm) | 0.32 |
| Correction Factor (280 nm) | 0.178 |
| Extinction coefficient (cm -1 M -1) | 83000 |
| Excitation (nm) | 493 |
| Emission (nm) | 517 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| FAM Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 493 | 517 | 83000 |
| VIC Dye qPCR Calibration Solution *10,000X* | 526 | 543 | - |
| NED Dye qPCR Calibration Solution *10,000X* | 545 | 567 | 740001 |
| Cy5 Dye qPCR Calibration Solution *10,000X* | 651 | 670 | 2500001 |
| ROX Dye qPCR Calibration Solution *10,000X* | 578 | 595 | - |
| SYBR Dye qPCR Calibration Solution *10,000X* | 498 | 522 | - |
| TAMRA Dye qPCR Calibration Solution *10,000X* | 552 | 578 | 90000 |
| ABY Dye qPCR Calibration Solution *10,000X* | 559 | 570 | 1200001 |
| JUN Dye qPCR Calibration Solution *10,000X* | 610 | 628 | 1100001 |
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Images
Application notes
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?