Name: FAM-VAD-FMK
Brand: AAT Bioquest
SKU: 13470
Availability: InStock

FAM-VAD-FMK

FAM-VAD is a green fluorescent cell-permeable polycaspase inhibitor to target caspases 1, 2, 3, 6, 8, 9, or 10. Once inside the cell, the inhibitor binds covalently to the active caspase, which produces a green fluorescence. When added to a population of cells, the FAM-VAD-FMK probe enters each cell and covalently binds to a reactive cysteine residue that resides on the large subunit of the active caspase heterodimer, thereby inhibiting further enzymatic activity. The bound labeled reagent is retained within the cell, while any unbound reagent will diffuse out of the cell and is washed away. The green fluorescent signal is a direct measure of the amount of active caspase present in the cell at the time the reagent was added. Cells that contain the bound labeled reagent can be analyzed by 96-well plate-based fluorometry, fluorescence microscopy, or flow cytometry. It works in diverse cell lines such as human, rodent and drosophila.

Protocol

COA

Catalog	Size	Price	Quantity
13470	25 Tests	Price
13484	100 Tests	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of FAM-VAD-FMK to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	144.58 µL	722.899 µL	1.446 mL	7.229 mL	14.458 mL
5 mM	28.916 µL	144.58 µL	289.159 µL	1.446 mL	2.892 mL
10 mM	14.458 µL	72.29 µL	144.58 µL	722.899 µL	1.446 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	691.66 g/mol	=	mL	x	mM	=	mmol

References

View all 26 references: Citation Explorer

Suppression of human T cell proliferation by the caspase inhibitors, z-VAD-FMK and z-IETD-FMK is independent of their caspase inhibition properties

Authors:

Lawrence CP, Chow SC.

Journal:

Toxicol Appl Pharmacol. (2012)

Inhibition of elicitation of allergic contact dermatitis by topical use of Z-VAD-FMK, a broad caspase inhibitor: experiment in mice

Authors:

Li YY, Yan CL.

Journal:

Zhonghua Yi Xue Za Zhi (2012): 1992

Structure of human caspase-6 in complex with Z-VAD-FMK: New peptide binding mode observed for the non-canonical caspase conformation

Authors:

Muller I, Lamers MB, Ritchie AJ, Dominguez C, Munoz-Sanjuan I, Kiselyov A.

Journal:

Bioorg Med Chem Lett (2011): 5244

Intracochlear perfusion of leupeptin and z-VAD-FMK: influence of antiapoptotic agents on gunshot-induced hearing loss

Authors:

Abaamrane L, Raffin F, Schmerber S, Sendowski I.

Journal:

Eur Arch Otorhinolaryngol (2011): 987

Plasmodium falciparum metacaspase PfMCA-1 triggers a z-VAD-fmk inhibitable protease to promote cell death

Authors:

Meslin B, Beavogui AH, Fasel N, Picot S.

Journal:

PLoS One (2011): e23867

Physical properties

Molecular weight	691.66
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 17, 2025