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FAM-xtra CPG *1000A*

Fluorescein CPGs have traditionally been used to add the fluorescein label at the 3'-terminus. FAM-xtra is a new fluorophore that has the identical spectra to FAM, but is much brighter and more photostable than FAM. FAM-xtra CPG is used to add the significantly improved FAM label at the 3'-terminus in complimentary to our FAM-xtra Phosphoramidite that contains no 4,4'-dimethoxytrityl (DMT) group and can be added only once at the 5'-terminus, thereby terminating synthesis. Both FAM-xtra products are single isomers and use an amide linkage which is stable during cleavage and deprotection and does not allow isomer formation. FAM-xtra CPG allows effective blockage of the 3'-terminus from polymerase extension as well as exonuclease digestion. This support can be used in a manner identical to normal protected nucleoside support since it contains the DMT group. It can be deprotected using ammonium hydroxide as required by nucleobases. When using AMA, a small amount of a non-fluorescent impurity might be formed. Alternatively, it may be first deprotected with ammonium hydroxide for 30 minutes at room temperature, followed by the addition of an equal volume of 40% methylamine. The deprotection is completed as required by the nucleobases - e.g. 10 minutes at 65°C or 2 hours at room temperature for standard bases etc.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
FAM-xtra™ Phosphoramidite493517830000.320.178

References

View all 50 references: Citation Explorer
Chemo-photothermal immunotherapy for eradication of orthotopic tumors and inhibition of metastasis by intratumoral injection of polydopamine versatile hydrogels.
Authors: Zhuang, Bo and Chen, Ting and Huang, Yueqi and Xiao, Zhimei and Jin, Yiguang
Journal: Acta pharmaceutica Sinica. B (2022): 1447-1459
Quantitative Pathologic Analysis of Digitized Images of Colorectal Carcinoma Improves Prediction of Recurrence-Free Survival.
Authors: Pai, Reetesh K and Banerjee, Imon and Shivji, Sameer and Jain, Suchit and Hartman, Douglas and Buchanan, Daniel D and Jenkins, Mark A and Schaeffer, David F and Rosty, Christophe and Como, Julia and Phipps, Amanda I and Newcomb, Polly A and Burnett-Hartman, Andrea N and Le Marchand, Loic and Samadder, Niloy J and Patel, Bhavik and Swallow, Carol and Lindor, Noralane M and Gallinger, Steven J and Grant, Robert C and Westerling-Bui, Thomas and Conner, James and Cyr, David P and Kirsch, Richard and Pai, Rish K
Journal: Gastroenterology (2022): 1531-1546.e8
Characterization of CD19+ CD24hi CD38hi B cells in Chinese adult patients with atopic dermatitis.
Authors: Gu, Y and Li, K and Sun, J and Zhang, J
Journal: Journal of the European Academy of Dermatology and Venereology : JEADV (2020): 2863-2870
Weak Electric Current Treatment to Artificially Enhance Vascular Permeability in Embryonated Chicken Eggs.
Authors: Fukuta, Tatsuya and Nakatani, Natsu and Yoneda, Shintaro and Kogure, Kentaro
Journal: Biological & pharmaceutical bulletin (2020): 1729-1734
Novel Assay for Quantitative Analysis of DNA Methylation at Single-Base Resolution.
Authors: Yu, Huichuan and Bai, Liangliang and Tang, Guannan and Wang, Xiaolin and Huang, Meijin and Cao, Guangwen and Wang, Jianping and Luo, Yanxin
Journal: Clinical chemistry (2019): 664-673
Page updated on December 1, 2024

Ordering information

Price
Unit size
100 mg
1 g
Catalog Number
60436044
Quantity
Add to cart

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Physical properties

Solvent

MeCN

Spectral properties

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.178

Extinction coefficient (cm -1 M -1)

83000

Excitation (nm)

493

Emission (nm)

517

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
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