FAM-xtra CPG *1000A*
Fluorescein CPGs have traditionally been used to add the fluorescein label at the 3'-terminus. FAM-xtra is a new fluorophore that has the identical spectra to FAM, but is much brighter and more photostable than FAM. FAM-xtra CPG is used to add the significantly improved FAM label at the 3'-terminus in complimentary to our FAM-xtra Phosphoramidite that contains no 4,4'-dimethoxytrityl (DMT) group and can be added only once at the 5'-terminus, thereby terminating synthesis. Both FAM-xtra products are single isomers and use an amide linkage which is stable during cleavage and deprotection and does not allow isomer formation. FAM-xtra CPG allows effective blockage of the 3'-terminus from polymerase extension as well as exonuclease digestion. This support can be used in a manner identical to normal protected nucleoside support since it contains the DMT group. It can be deprotected using ammonium hydroxide as required by nucleobases. When using AMA, a small amount of a non-fluorescent impurity might be formed. Alternatively, it may be first deprotected with ammonium hydroxide for 30 minutes at room temperature, followed by the addition of an equal volume of 40% methylamine. The deprotection is completed as required by the nucleobases - e.g. 10 minutes at 65°C or 2 hours at room temperature for standard bases etc.
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
FAM-xtra™ Phosphoramidite | 493 | 517 | 83000 | 0.32 | 0.178 |
References
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Chemo-photothermal immunotherapy for eradication of orthotopic tumors and inhibition of metastasis by intratumoral injection of polydopamine versatile hydrogels.
Authors: Zhuang, Bo and Chen, Ting and Huang, Yueqi and Xiao, Zhimei and Jin, Yiguang
Journal: Acta pharmaceutica Sinica. B (2022): 1447-1459
Authors: Zhuang, Bo and Chen, Ting and Huang, Yueqi and Xiao, Zhimei and Jin, Yiguang
Journal: Acta pharmaceutica Sinica. B (2022): 1447-1459
Quantitative Pathologic Analysis of Digitized Images of Colorectal Carcinoma Improves Prediction of Recurrence-Free Survival.
Authors: Pai, Reetesh K and Banerjee, Imon and Shivji, Sameer and Jain, Suchit and Hartman, Douglas and Buchanan, Daniel D and Jenkins, Mark A and Schaeffer, David F and Rosty, Christophe and Como, Julia and Phipps, Amanda I and Newcomb, Polly A and Burnett-Hartman, Andrea N and Le Marchand, Loic and Samadder, Niloy J and Patel, Bhavik and Swallow, Carol and Lindor, Noralane M and Gallinger, Steven J and Grant, Robert C and Westerling-Bui, Thomas and Conner, James and Cyr, David P and Kirsch, Richard and Pai, Rish K
Journal: Gastroenterology (2022): 1531-1546.e8
Authors: Pai, Reetesh K and Banerjee, Imon and Shivji, Sameer and Jain, Suchit and Hartman, Douglas and Buchanan, Daniel D and Jenkins, Mark A and Schaeffer, David F and Rosty, Christophe and Como, Julia and Phipps, Amanda I and Newcomb, Polly A and Burnett-Hartman, Andrea N and Le Marchand, Loic and Samadder, Niloy J and Patel, Bhavik and Swallow, Carol and Lindor, Noralane M and Gallinger, Steven J and Grant, Robert C and Westerling-Bui, Thomas and Conner, James and Cyr, David P and Kirsch, Richard and Pai, Rish K
Journal: Gastroenterology (2022): 1531-1546.e8
Characterization of CD19+ CD24hi CD38hi B cells in Chinese adult patients with atopic dermatitis.
Authors: Gu, Y and Li, K and Sun, J and Zhang, J
Journal: Journal of the European Academy of Dermatology and Venereology : JEADV (2020): 2863-2870
Authors: Gu, Y and Li, K and Sun, J and Zhang, J
Journal: Journal of the European Academy of Dermatology and Venereology : JEADV (2020): 2863-2870
Weak Electric Current Treatment to Artificially Enhance Vascular Permeability in Embryonated Chicken Eggs.
Authors: Fukuta, Tatsuya and Nakatani, Natsu and Yoneda, Shintaro and Kogure, Kentaro
Journal: Biological & pharmaceutical bulletin (2020): 1729-1734
Authors: Fukuta, Tatsuya and Nakatani, Natsu and Yoneda, Shintaro and Kogure, Kentaro
Journal: Biological & pharmaceutical bulletin (2020): 1729-1734
Novel Assay for Quantitative Analysis of DNA Methylation at Single-Base Resolution.
Authors: Yu, Huichuan and Bai, Liangliang and Tang, Guannan and Wang, Xiaolin and Huang, Meijin and Cao, Guangwen and Wang, Jianping and Luo, Yanxin
Journal: Clinical chemistry (2019): 664-673
Authors: Yu, Huichuan and Bai, Liangliang and Tang, Guannan and Wang, Xiaolin and Huang, Meijin and Cao, Guangwen and Wang, Jianping and Luo, Yanxin
Journal: Clinical chemistry (2019): 664-673
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