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FAM-xtra™ Phosphoramidite
There are several ways of labeling an oligonucleotide with fluorescein. 6-FAM phosphoramidite might be the most popular fluorescent building block that is widely used to efficiently label an oligonucleotide at the 5’-end with the fluorescein fluorophore. Standard cleavage and deprotection with ammonium hydroxide are used to liberate the fluorescein-labeled oligos. Although 6-FAM has be ubiquitously used for developing a variety of qPCR probes, its fluorescence suffers two serve limitations: a). highly pH dependent; and b). photo-bleach rapidly. FAM-xtra™ Phosphoramidite has been developed to address these two limitations while the well-beloved operational convenience of 6-FAM phosphoramidite is intactly maintained. FAM-xtra Phosphoramidite can be used exactly as is used for 6-FAM phosphoramidite under the same operation conditions without any changes needed. The oligos made from FAM-xtra Phosphoramidite have quite a few great benefits: a). FAM-xtra-labeled oligos have the identical spectra to those of 6-FAM labeled ones; b). FAM-xtra-labeled oligos are much brighter than the corresponding FAM-labeled ones; c). FAM-xtra-labeled oligos have much higher photostability than the corresponding FAM-labeled ones; and d). The fluorescence of FAM-xtra-labeled oligos is much less pH sensitive than that of the corresponding FAM-labeled ones. Under the physiological conditions, the fluorescence of FAM-xtra-labeled oligos is essentially pH-independent, making the FAM-xtra-labeled oligos much more robust qPCR probes.
Chemical structure for FAM-xtra™ Phosphoramidite.
Chemical structure for FAM-xtra™ Phosphoramidite.
CatalogSize
Price
Quantity
603750 umoles
Price
6038100 umoles
Price
603910x100 umoles
Price
 
Physical properties

Molecular weight879.94
SolventMeCN
Spectral properties

Correction factor (260 nm)0.32
Correction factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)
83000
Excitation (nm)493
Emission (nm)517
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 14, 2025