AAT Bioquest

FastClick™ 5-FAM Alkyne

The reaction (Green Bar) of FastClick Cy5 Alkyne with coumarin azide occurs under extremely mild conditions (e.g., [Azide] = 0.02 mM, [Alkyne] = 0.02 mM, [CuSO4] = 0.02 mM, [Sodium Ascorbate] = 5 mM, in 100 mM HEPES) under which the common Cy5 alkyne does not effectively react with the coumarin azide substrate.
The reaction (Green Bar) of FastClick Cy5 Alkyne with coumarin azide occurs under extremely mild conditions (e.g., [Azide] = 0.02 mM, [Alkyne] = 0.02 mM, [CuSO4] = 0.02 mM, [Sodium Ascorbate] = 5 mM, in 100 mM HEPES) under which the common Cy5 alkyne does not effectively react with the coumarin azide substrate.
The reaction (Green Bar) of FastClick Cy5 Alkyne with coumarin azide occurs under extremely mild conditions (e.g., [Azide] = 0.02 mM, [Alkyne] = 0.02 mM, [CuSO4] = 0.02 mM, [Sodium Ascorbate] = 5 mM, in 100 mM HEPES) under which the common Cy5 alkyne does not effectively react with the coumarin azide substrate.
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Physical properties
Molecular weight684.75
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Alternative formats
FastClick™ 5-FAM Azide
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Show More (43)


See also: Click Chemistry
Molecular weight
FastClick™ 5-FAM Alkyne contains both the moiety of FastClick (for assisting click efficiency) and 5-FAM fluorophore (as the fluorescence tag) for developing 5-FAM-based fluorescent probes. It readily reacts with an azido-modified biomolecule under extremely mild conditions. 5-FAM is one of the most common green fluorophores used for labeling biomolecules, in particular, for labeling peptides. 5-FAM conjugates are widely used in fluorescence-based biological detections. FastClick™ reagents have been developed by the scientists of AAT Bioquest for enhancing the yield and reaction speed of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. They contain a copper-chelating ligand that significantly stabilizes the Cu(I) oxidation state and thus accelerates the click reaction. They do not require the use of an external copper-chelator (such as the common THPTA or BTTAA). The high concentration of copper chelators is known to have a detrimental effect on DNA/RNA, thus causing biocompatibility issues. The introduction of a copper-chelating moiety at the reporter molecule allows for a dramatic raise of the effective Cu(I) concentration at the reaction site and thus accelerates the reaction. Under extremely mild conditions the FastClick™ azides and alkynes react much faster in high yield compared to the corresponding conventional CuAAC reactions. Click chemistry was developed by K. Barry Sharpless as a robust and specific method of ligating two molecules together. Two important characteristics make click chemistry attractive for assembling biomolecules. First, click reactions are bio-orthogonal, thus the click chemistry-functionalized biomolecules would not react with the natural biomolecules that lack a clickable functional group. Second, the reactions proceed with ease under mild conditions, such as at room temperature and in aqueous media.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of FastClick™ 5-FAM Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM146.039 µL730.194 µL1.46 mL7.302 mL14.604 mL
5 mM29.208 µL146.039 µL292.077 µL1.46 mL2.921 mL
10 mM14.604 µL73.019 µL146.039 µL730.194 µL1.46 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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View all 26 references: Citation Explorer
Programmed immobilization of living cells using independent click pairs.
Authors: Zhu, Chengyuan and Takemoto, Hiroyasu and Higuchi, Yuriko and Yamashita, Fumiyoshi
Journal: Biochemical and biophysical research communications (2024): 149556
Synthesis of bio-templated clickable quantum dots and a dual-emitting organic/inorganic complex for ratiometric fluorescence visual assay of blood glucose.
Authors: Yang, Yeling and Mao, Guobin and Ji, Xinghu and He, Zhike
Journal: Journal of materials chemistry. B (2022): 4473-4478
Design and Synthesis of Fluorophore-Tagged Disparlure Enantiomers to Study Pheromone Enantiomer Discrimination in the Pheromone-Binding Proteins from the Gypsy Moth, Lymantria dispar.
Authors: Pinnelli, Govardhana R and Plettner, Erika
Journal: Journal of chemical ecology (2021)
Rapid stimulation of cellular Pi uptake by the inositol pyrophosphate InsP8 induced by its photothermal release from lipid nanocarriers using a near infra-red light-emitting diode.
Authors: Wang, Zhenzhen and Jork, Nikolaus and Bittner, Tamara and Wang, Huanchen and Jessen, Henning J and Shears, Stephen B
Journal: Chemical science (2020): 10265-10278
Fluorescent Properties of Carboxyfluorescein Bifluorophores.
Authors: Povedailo, Vladimir A and Lysenko, Ivan L and Tikhomirov, Sergei A and Yakovlev, Dmitrii L and Tsybulsky, Dmitry A and Kruhlik, Aliaksandr S and Fan, Fan and Martynenko-Makaev, Yury V and Sharko, Olga L and Duong, Pham V and Minh, Pham H and Shmanai, Vadim V
Journal: Journal of fluorescence (2020): 629-635
A mid-Cretaceous trichomycete, Priscadvena corymbosa gen. et sp. nov., in Burmese amber.
Authors: Poinar, George and Vega, Fernando E
Journal: Fungal biology (2019): 393-396
Click Chemistry on the Surface of Ultrasmall Gold Nanoparticles (2 nm) for Covalent Ligand Attachment Followed by NMR Spectroscopy.
Authors: van der Meer, Selina Beatrice and Loza, Kateryna and Wey, Karolin and Heggen, Marc and Beuck, Christine and Bayer, Peter and Epple, Matthias
Journal: Langmuir : the ACS journal of surfaces and colloids (2019): 7191-7204
Gold Nanoparticles Functionalized with RGD-Semipeptides: A Simple yet Highly Effective Targeting System for αV β3 Integrins.
Authors: Maggi, Vito and Bianchini, Francesca and Portioli, Elisabetta and Peppicelli, Silvia and Lulli, Matteo and Bani, Daniele and Sole, Roberta Del and Zanardi, Franca and Sartori, Andrea and Fiammengo, Roberto
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2018): 12093-12100
Just a click away: exploring patients' perspectives on receiving care through the Champlain BASETM eConsult service.
Authors: Joschko, Justin and Liddy, Clare and Moroz, Isabella and Reiche, Marnie and Crowe, Lois and Afkham, Amir and Keely, Erin
Journal: Family practice (2018): 93-98
Stopwatches and Click Boxes: The Intersection of Scientific Management and Family Medicine.
Authors: Ledford, Christy J W
Journal: Family medicine (2018): 89-90