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FITC-xtra

HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin and biotin goat anti-mouse IgG followed by FITC-Xtra streptavidin conjugate (Green, Left) or FITC-streptavidin conjugate (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin and biotin goat anti-mouse IgG followed by FITC-Xtra streptavidin conjugate (Green, Left) or FITC-streptavidin conjugate (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
Ordering information
Price ()
Catalog Number135
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight620.52
SolventDMSO
Spectral properties
Excitation (nm)498
Emission (nm)526
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
620.52
Excitation (nm)
498
Emission (nm)
526
Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with FITC-xtra are far superior compared to the corresponding FITC conjugates. FITC-xtra conjugates are significantly brighter than FITC conjugates and are much more photostable. Additionally, the fluorescence of FITC-xtra is not affected by pH (4-10). This pH insensitivity is a major improvement over FITC, which emits its maximum fluorescence only at pH above 9. FITC-xtra has spectral properties almost identical FITC. In addition, FITC-xtra give much higher conjugation yield under mild conjugation conditions than FITC. Like 5-FITC, FITC-xtra antibody conjugates have excitation ideally suited to the 488 nm laser line, making them alternatives to the corresponding FITC-labeled antibody conjugates. Under the same conditions tested, FITC-xtra antibody conjugates give much higher signal/background ratios than the corresponding FITC-labeled conjugates.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of FITC-xtra to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM161.155 µL805.776 µL1.612 mL8.058 mL16.116 mL
5 mM32.231 µL161.155 µL322.31 µL1.612 mL3.223 mL
10 mM16.116 µL80.578 µL161.155 µL805.776 µL1.612 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Excitation (nm)498
Emission (nm)526

Citations


View all 3 citations: Citation Explorer
Determination of sodium benzoate in food products by fluorescence polarization immunoassay
Authors: Ren, Linlin and Meng, Meng and Wang, Peng and Xu, Zhihuan and Eremin, Sergei A and Zhao, Junhong and Yin, Yongmei and Xi, Rimo
Journal: Talanta (2014): 136--143
Complexes containing cationic and anionic pH-sensitive liposomes: comparative study of factors influencing plasmid DNA gene delivery to tumors
Authors: Chen, Yan and Sun, Ji and Lu, Ying and Tao, Chun and Huang, Jingbin and Zhang, He and Yu, Yuan and Zou, Hao and Gao, Jing and Zhong, Yanqiang
Journal: International journal of nanomedicine (2013): 1573
Swimming behavior of the monotrichous bacterium Pseudomonas fluorescens SBW25
Authors: Ping, Liyan and Birkenbeil, Jan and Monajembashi, Shamci
Journal: FEMS microbiology ecology (2013): 36--44

References


View all 103 references: Citation Explorer
Isothiocyanato Boron Dipyrromethenes - The First BODIPY Analogues of Fluorescein Isothiocyanate (FITC)
Authors: Malatesti N, Hudson R, Smith K, Savoie H, Rix K, Welham K, Boyle RW.
Journal: Photochem Photobiol. (2006)
Detection of FITC-cortisol via modulated supraparticle lighthouses
Authors: Petkus MM, McLauchlin M, Vuppu AK, Rios L, Garcia AA, Hayes MA.
Journal: Anal Chem (2006): 1405
Clathrin-mediated endocytosis of FITC-albumin in alveolar type II epithelial cell line RLE-6TN
Authors: Yumoto R, Nishikawa H, Okamoto M, Katayama H, Nagai J, Takano M.
Journal: Am J Physiol Lung Cell Mol Physiol (2006): L946
Fluorescein isothiocyanate labelled bovine serum albumin (FITC-BSA) as a model protein drug: opportunities and drawbacks
Authors: Wischke C, Borchert HH.
Journal: Pharmazie (2006): 770
Increased FITC fluorescence on LPS stimulated neutrophils cultured in whole blood
Authors: Wilkins RC, Bellier PV, Kutzner BC, McNamee JP.
Journal: Cell Biol Int (2006): 394
Effects of pretreatment of needle puncture and sandpaper abrasion on the in vitro skin permeation of fluorescein isothiocyanate (FITC)-dextran
Authors: Wu XM, Todo H, Sugibayashi K.
Journal: Int J Pharm (2006): 102
Uptake of dextran-FITC by epithelial cells of the chorioallantoic placentome and the omphalopleure of the placentotrophic lizard, Pseudemoia entrecasteauxii
Authors: Stewart JR, Thompson MB, Attaway MB, Herbert JF, Murphy CR.
Journal: J Exp Zoolog A Comp Exp Biol (2006): 883
Intracellular FITC-derivatization with PEG
Authors: Chen F, Zhi D, Xia G.
Journal: Electrophoresis (2005): 4204
Potential determination of aminated pesticides and metabolites by cyclodextrin capillary electrophoresis-laser-induced fluorescence using FITC as labelling
Authors: Navarrete-Casas M, Segura-Carretero A, Cruces-Blanco C, Fern and ez-Gutierrez A., undefined
Journal: Pest Manag Sci (2005): 197
Preparation and characterization of a directly labeled mouse anti-human CD14 monoclonal antibody ZCH-2F9-FITC.
Authors: Ning BT, Tang YM, Shen HQ, Yang SL, Chen YH, Song H, Shi SW, Qian BQ, Xu WQ.
Journal: Zhejiang Da Xue Xue Bao Yi Xue Ban (2005): 167